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Protective Effects of New Herbal Composition (MH-30) against Radiation Injuries in Hematopoietic and Self-Renewal Tissues
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 Title & Authors
Protective Effects of New Herbal Composition (MH-30) against Radiation Injuries in Hematopoietic and Self-Renewal Tissues
Jung, Uhee; Park, Hae-Ran; Lee, Ho-Yong; Baek, Ga-Young; Jo, Sung-Kee;
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We previously developed an herbal composition (HemoHIM) based on the water extracts of Angelica gigas radix, Cnidium officinale rhizoma, and Paeonia japonica radix to protect and recover hematopoietic and intestinal tissues against radiation injuries. In this study, to develop a composition with improved activities based on enhanced fat-soluble polyphenol contents, we prepared a new herbal composition, MH-30, from the above three herbs by 30% ethanol extraction and hot water extraction. HPLC analysis of the ethanol fractions of MH-30 and HemoHIM revealed that MH-30 had higher contents of many fat-soluble polyphenol compounds than HemoHIM (8.7-fold increase for decursin), whereas contents of water-soluble polyphenol compounds showed little differences between the two compositions. Then, we evaluated MH-30 and HemoHIM for their in vitro antioxidant and immune cell-stimulating activities as well as in vivo protective effects against radiation injuries in hematopoietic and self-renewal tissues. In antioxidant activity assays, MH-30 showed higher hydroxyl radical scavenging activity than HemoHIM (1.4- to 1.9-fold for compositions and 2.3- to 4.5-fold for ethanol fractions). On the other hand, MH-30 and HemoHIM exhibited similar immune cell-stimulating activities as measured by in vitro lymphocyte proliferation. MH-30 increased endogenous spleen colony formation, decreased bone marrow cell apoptosis, and enhanced survival of intestinal crypts in irradiated mice, demonstrating effective protection of MH-30 against radiation-induced injuries in hematopoietic and self-renewal tissues. The 30-day survival rate of lethally irradiated mice, a comprehensive index for radioprotective efficacy, was also elevated by MH-30. Noticeably, MH-30 showed higher protective effects than HemoHIM in all mouse experiments. These results demonstrate that MH-30 can protect hematopoietic and self-renewal tissues against radiation injuries more effectively than HemoHIM. Therefore, MH-30 can be a good candidate to reduce radiation injuries in hematopoietic and self-renewal tissues incurred by radiation accidents or cancer radiation therapy.
herbal composition;radiation protection;hematopoiesis;immune;self-renewal tissue;
 Cited by
Singh VK, Romaine PL, Seed TM. 2015. Medical Countermeasures for radiation exposure and related injuries: Characterization of medicines, FDA-approval status and inclusion into the strategic national stockpile. Health Phys 108: 607-630. crossref(new window)

Halliwell B, Gutteridge JM. 1999. Free radicals in biology and medicine. 3rd ed. Oxford University Press, New York, NY, USA. p 604-607.

Hendry JH, Roberts SA, Potten CS. 1992. The clonogen content of murine intestinal crypts: dependence on radiation dose used in its determination. Radiat Res 132: 115-119. crossref(new window)

Schwartz GN, Neta R, Vigneulle RM, Patchen ML, MacVittie TJ. 1988. Recovery of hematopoietic colony-forming cells in irradiated mice pretreated with interleukin 1 (IL-1). Exp Hematol 16: 752-757.

Travis EL, Fang MZ, Basic I. 1988. Protection of mouse bone marrow by WR-2721 after fractionated irradiation. Int J Radiat Oncol Biol Phys 15: 377-382. crossref(new window)

Milas L, Hunter N, Reid BO, Thames HD Jr. 1982. Protective effects of S-2-(3-aminopropylamino)ethylphosphorothioic acid against radiation damage of normal tissues and a fibrosarcoma in mice. Cancer Res 42: 1888-1987.

Milas L, Murray D, Brock WA, Meyn RE. 1988. Radioprotectors in tumor radiotherapy: factors and settings determining therapeutic ratio. Pharmacol Ther 39: 179-187. crossref(new window)

Neta R, Douches S, Oppenheim JJ. 1986. Interleukin 1 is a radioprotector. J Immunol 136: 2483-2485.

Neta R. 1988. Role of cytokines in radioprotection. Pharmacol Ther 39: 261-266. crossref(new window)

MacVittie TJ, Monroy RL, Patchen ML, Souza LM. 1990. Therapeutic use of recombinant human G-CSF (rhG-CSF) in a canine model of sublethal and lethal whole-body irradiation. Int J Radiat Biol 57: 723-736. crossref(new window)

Satoh S, Suzuki A, Okamura H, Nishimura T. 1982. Case of malignant melanoma of the external genitalia responding satisfactorily to a combination of local injection of OK-432 and chemotherapy. Gan To Kagaku Ryoho 9: 140-145.

Bogo V, Jacobs AJ, Weiss JF. 1985. Behavioral toxicity and efficacy of WR-2721 as a radioprotectant. Radiat Res 104: 182-190. crossref(new window)

Rades D, Fehlauer F, Bajrovic A, Mahlmann B, Richter E, Alberti W. 2004. Serious adverse effects of amifostine during radiotherapy in head and neck cancer patients. Radiother Oncol 70: 261-264. crossref(new window)

Miyanomae T, Frindel E. 1988. Radioprotection of hemopoiesis conferred by Acanthopanax senticosus Harms (Shigoka) administered before or after irradiation. Exp Hematol 16: 801-806.

Hsu HY, Lian SL, Lin CC. 1990. Radioprotective effect of Ganoderma lucidum (Leyss. ex. Fr.) Karst after X-ray irradiation in mice. Am J Chin Med 18: 61-69. crossref(new window)

Mei QB, Tao TY, Cui B. 1991. Advances in the pharmacological studies of radix Angelica sinensis (Oliv) Diels (Chinese Danggui). Chin Med J (Engl) 104: 776-781.

Ohta S, Sakurai N, Sato Y, Inoue T, Shinoda M. 1990. Studies on chemical protectors against radiation. XXX. Radioprotective substances of Cnidii rhizoma. Yakugaku Zasshi 110: 746-754. crossref(new window)

Zneg XL, Li XA, Zhang BY. 1992. Immunological and hematopoietic effect of Codonopsis pilosula on cancer patients during radiotherapy. Zhongguo Zhong Xi Yi Jie He Za Zhi 12: 607-608.

Quan HX, Li HS. 1994. Effects of radix Astragali on hemopoiesis in irradiated mice. Zhongguo Zhong Yao Za Zhi 19: 741-743.

Kim SH, Lee SE, Oh H, Kim SR, Yee ST, Yu YB, Byun MW, Jo SK. 2002. The radioprotective effects of bu-zhongyi-qi-tang: a prescription of traditional Chinese medicine. Am J Chin Med 30: 127-137. crossref(new window)

Hisha H, Yamada H, Sakurai MH, Kiyohara H, Li Y, Yu C, Takemoto N, Kawamura H, Yamaura K, Shinohara S, Komatsu Y, Aburada M, Ikehara S. 1997. Isolation and identification of hematopoietic stem cell-stimulating sub stances from Kampo (Japanese herbal) medicine, Juzen-taiho-to. Blood 90: 1022-1030.

Hsu HY, Hau DM, Lin CC. 1993. Effects of kuei-pi-tang on cellular immunocompetence of gamma-irradiated mice. Am J Chin Med 21: 151-158. crossref(new window)

Hsu HY, Ho YH, Lin CC. 1996. Protection of mouse bone marrow by Si-WU-Tang against whole body irradiation. J Ethnopharmacol 52: 113-117. crossref(new window)

Lee SE, Oh H, Yang JA, Jo SK, Byun MW, Yee ST, Kim SH. 1999. Radioprotective effects of two traditional Chinese medicine prescriptions: si-wu-tang and si-jun-zi-tang. Am J Chin Med 27: 387-396. crossref(new window)

Park HR, Kim SH, Yee ST, Byun MW, Jo SK. 2005. The effects of a herb mixture (HIM-I) on the protection of the hematopoietic-immune system and self-renewal tissues against radiation damage. J Korean Soc Food Sci Nutr 34: 605-612. crossref(new window)

Jo SK, Park HR, Jung U, Oh H, Kim SH, Yee ST. 2005. Protective effect of a herbal preparation (HemoHIM) on the self-renewal tissues and immune system against ${\gamma}$-irradiation. J Korean Soc Food Sci Nutr 34: 805-813. crossref(new window)

Kim SH, Lee HJ, Kim JS, Moon C, Kim JC, Park HR, Jung U, Jang JS, Jo SK. 2009. Protective effect of an herbal preparation (HemoHIM) on radiation-induced intestinal injury in mice. J Med Food 12: 1353-1358. crossref(new window)

Park HR, Jo SK, Jung U, Yee ST, Kim SH. 2014. Protective effects of HemoHIM on immune and hematopoietic systems against ${\gamma}$-irradiation. Phytother Res 28: 245-251. crossref(new window)

Park HR, Jo SK, Choi NH, Jung U. 2013. HemoHIM ameliorates the persistent down-regulation of Th1-like immune responses in fractionated ${\gamma}$-irradiated mice by modulating the IL-12p70-STAT4 signaling pathway. Radiat Res 177: 676-684.

Park HR, Ju EJ, Jo SK, Jung U, Kim SH. 2010. HemoHIM enhances the therapeutic efficacy of ionizing radiation treatment in tumor-bearing mice. J Med Food 13: 47-53. crossref(new window)

Park HR, Ju EJ, Jo SK, Jung U, Kim SH, Yee ST. 2009. Enhanced antitumor efficacy of cisplatin in combination with HemoHIM in tumor-bearing mice. BMC Cancer 9: 85. crossref(new window)

Park HR, Jo SK, Jung U, Yee ST. 2008. Restoration of the immune functions in aged mice by supplementation with a new herbal composition, HemoHIM. Phytother Res 22: 36-42. crossref(new window)

Kim JJ, Cho HW, Park HR, Jung U, Jo SK, Yee ST. 2013. Preventative effect of an herbal preparation (HemoHIM) on development of airway inflammation in mice via modulation of Th1/2 cells differentiation. PLoS ONE 8: e68552. crossref(new window)

Chaudhary S, Chandrashekar KS, Pai KS, Setty MM, Devkar RA, Reddy ND, Shoja MH. 2015. Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma. BMC Complement Altern Med 15: 50. crossref(new window)

Asghar N, Naqvi SA, Hussain Z, Rasool N, Khan ZA, Shahzad SA, Sherazi TA, Janjua MR, Nagra SA, Zia-Ul-Haq M, Jaafar HZ. 2016. Compositional difference in antioxidant and antibacterial activity of all parts of the Carica papaya using different solvents. Chem Cent J 10: 5. crossref(new window)

Gutteridge JM. 1984. Reactivity of hydroxyl and hydroxyllike radicals discriminated by release of thiobarbituric acidreactive material from deoxy sugars, nucleosides and benzoate. Biochem J 15: 761-767.

Peskin AV, Winterbourn CC. 2000. A microtiter plate assay for superoxide dismutase using a water-soluble tetrazolium salt (WST-1). Clin Chim Acta 293: 157-166. crossref(new window)

Milas L, Hunter N, Ito H, Peters LJ. 1984. In vivo radioprotective activities of diethyldithiocarbamate (DDC). Int J Radiat Oncol Biol Phys 10: 2335-2343. crossref(new window)

Duke RC, Chervenak R, Cohen JJ. 1983. Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis. Proc Natl Acad Sci U S A 80: 6361-6315. crossref(new window)

Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C. 1991. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 139: 271-279. crossref(new window)

Withers HR, Elkind MM. 1970. Microcolony survival assay for cells of mouse intestinal mucosa exposed to radiation. Int J Radiat Biol Relat Stud Phys Chem Med 17: 261-267. crossref(new window)

Hall EJ. 1988. Radiobiology for the radiologist. 3rd ed. J.B. Lippincott Company, Philadelphia, PA, USA. p 365-376.

Pamujula S, Kishore V, Rider B, Fermin CD, Graves RA, Agrawal KC, Mandal TK. 2005. Radioprotection in mice following oral delivery of amifostine nanoparticles. Int J Radiat Biol 81: 251-257. crossref(new window)

Ghosh SP, Kulkarni S, Perkins MW, Hieber K, Pessu RL, Gambles K, Maniar M, Kao TC, Seed TM, Kumar KS. 2012. Amelioration of radiation-induced hematopoietic and gastrointestinal damage by Ex-RAD(R) in mice. J Radiat Res 53: 526-536. crossref(new window)

Nair GG, Nair CK. 2013. Radioprotective effects of gallic acid in mice. Biomed Res Int 2013: 953079.

Singh VK, Hauer-Jensen M. 2016. ${\gamma}$-Tocotrienol as a promising countermeasure for acute radiation syndrome: Current Status. Int J Mol Sci 37: E663.

Verma P, Jahan S, Kim TH, Goyal PK. 2011. Management of radiation injuries by panax ginseng extract. J Ginseng Res 35: 261-271. crossref(new window)

Jothy SL, Saito T, Kanwar JR, Chen Y, Aziz A, Yin-Hui L, Sasidharan S. 2016. Radioprotective activity of Polyalthia longifolia standardized extract against X-ray radiation injury in mice. Phys Med 32: 150-161.

Jiang S, Shen X, Liu Y, He Y, Jiang D, Chen W. 2015. Radioprotective effects of Sipunculus nudus L. polysaccharide combined with WR-2721, rhIL-11 and rhG-CSF on radiation-injured mice. J Radiat Res 56: 515-522. crossref(new window)