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Antioxidant and Neuroprotective Effects of Green Tea Seed Shell Ethanol Extracts
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 Title & Authors
Antioxidant and Neuroprotective Effects of Green Tea Seed Shell Ethanol Extracts
Sung, Nak-Yun; Song, Hayeon; Ahn, Dong-Hyun; Yoo, Yung-Choon; Byun, Eui-Baek; Jang, Beom-Su; Park, Chulhwan; Park, Won-Jong; Byun, Eui-Hong;
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The objective of this study was to evaluate the antioxidant activity of green tea seed shell as an industrial byproduct. Green tea seed shell extract (GTSSE) was obtained by ethanol extraction, and the yield was . The radical scavenging activities [1,1-diphenyl-picrylhydrazyl and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)], xanthine oxidase inhibition activity, and reducing power of GTSSE dose-dependently increased. To estimate the neuroprotective effect of GTSSE, viability was tested in HT22 mouse hippocampal cells. GTSSE treatment induced cytotoxicity at a concentration higher than but not at a concentration lower than . Using this optimal concentration range, GTSSE treatment significantly increased cell viability in -treated HT22 cells. Further, GTSSE treatment increased superoxide dismutase activity and decreased the malonaldehyde level, a product of lipid peroxidation, in HT22 cells. Therefore, these results indicate that green tea seed shell extract may be useful for the development of antioxidant materials and have potential activity to prevent and treat neuro-degenerative diseases such as Alzheimer's disease.
anti-oxidant activity;green tea seed shell;HT22 hippocampal neuronal cells;cell viability;neuroprotective effect;
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