Advanced SearchSearch Tips
Neuronal Cell Protective Effects of Hot Water Extracts from Guava (Psidium guajava L.) Fruit and Leaf
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Food Preservation
  • Volume 18, Issue 1,  2011, pp.124-129
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2011.18.1.124
 Title & Authors
Neuronal Cell Protective Effects of Hot Water Extracts from Guava (Psidium guajava L.) Fruit and Leaf
Jeong, Chang-Ho; Jeong, Hee-Rok; Choi, Gwi-Nam; Kwak, Ji-Hyun; Kim, Ji-Hye; Park, Soo-Jeong; Kim, Dae-Ok; Shim, Ki-Hwan; Choi, Sung-Gil; Heo, Ho-Jin;
  PDF(new window)
PC12 neuronal cell-protective effects of hot water extracts of guava fruit and leaf were evaluated. Total phenolic levels in fruit and leaf were 11.75 and 293.25 mg/g, respectively. Gallic acid, the predominant phenoic, was detected in both extracts. Intracellular reactive oxygen species (ROS) accumulation after treatment was significantly reduced when the hot water extract of guava leaf was added to cell medium, compared to PC12 cells treated with only. In a cell viability assay using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl- tetrazoliumbromide (MTT), the hot water extracts of fruit and leaf protected against -induced neurotoxicity. The leaf extract was more effective in terms of inhibition of lactate dehydrogenase (LDH) release into medium, compared to the fruit extract. These in vitro data suggest that hot water extracts of guava fruit and leaf may be useful in treatment of neurodegenerative conditions such as Alzheimer's disease.
PC12;neuronal cell-protective effect;guava;antioxidant activity;gallic acid;
 Cited by
구아바 잎 열수추출물의 당류분해효소 저해 효과 및 OGTT에 미치는 효과,최수민;김중학;정지상;김화영;장혜은;황성주;홍성길;

한국약용작물학회지, 2012. vol.20. 2, pp.94-100 crossref(new window)
Effect of Aqueous Extract of Guava (Psidium guajava L.) Leaves on the Oral Glucose Tolerance Test and Inhibition of Glycoside Hydrolase, Korean Journal of Medicinal Crop Science, 2012, 20, 2, 94  crossref(new windwow)
Runyons CR, Schmitt FA, Caban-Holt A, Kryscio RJ, Mendiondo MS, Markesbery WR (2005) Antioxidants for prevention of dementia : overview of the pre-advise trial. Alzheimer's Dementia, 1, 74 crossref(new window)

Behl C (1999) Alzheimer's disease and oxidative stress: implications for novel therapeutic approaches. Prog Neurobiol, 57, 301-323 crossref(new window)

Ames BN, shigenaga MK, Hagen TM (1993) Oxidant, antioxidants, and the degenerative disease of aging. Proc Natl Acad Sci USA, 90, 7915-7922 crossref(new window)

Heo HJ, Choi SJ, Choi SG, Shin DH, Lee JM, Lee CY (2008) Effects of banana, orange, and apple on oxidative stress-induced neurotoxicity in PC12 Cells. J Food Sci, 73, 28-32 crossref(new window)

Begum S, Hassa SI, Siddiqui BS, Shaheen F, Ghayur MM, Gilani AH (2002) Triterpenoids from the leaves of Psidium guajava. Phytochemistry, 61, 399-403 crossref(new window)

Lozoya X, Reyes-Morales H, Chávez-Soto MA, Martínez-Garcia Mdel. C, Soto-González Y, Doubova, SV (2002) Intestinal anti-spasmodic effect of a phytodrug of Psidium guajava folia in the treatment of acute diarrheic disease. J Ethnopharmacol, 83, 19-24 crossref(new window)

Misra K, Seshadri TR (1968) Chemical components of the fruits of Psidium guajava. Phytochemistry, 7, 641-645 crossref(new window)

Mercadante AZ, Steck A, Pfander H (1999) Carotenoids from guava (Psidium guajava L.) : isolation and structure elucidation. J Agric Food Chem, 47, 145-151 crossref(new window)

Miean KH, Mohamed S (2001) Flavonoid (myricetin, quercetin kaempferol, luteolin, and apigenin) content of edible tropical plants. J Agric Food Chem, 49, 3106-3112 crossref(new window)

Jaiarj P, Wongkrajang Y, Thongpraditchote S, Peungvicha P, Bunyapraphatsara N, Opartkiattikul N (2000) Guava leaf extract and topical haemostasis. Phytother Res, 14, 388-391 crossref(new window)

Jin YJ, Kang SH, Choi SY Park SY, Park JG, Moon SW, Park DB, Kim SJ (2006) Effect of fermented guava (Psidium guajava L.) leaf extract on hyperglycemia in low dose streptozotocin-induced diabetic mice. Korean J Food Sci Technol, 38, 679-683

Roh SG, Kim KH, Cho WC (2009) Antidiabetic effects of leaves extracts of Psidium guajava L. and Lagerstroemia speciosa L. in STZ-induced rats. J Life Sci, 19, 40-45 crossref(new window)

Hong CS, Yoon SR, Lee GD, Kim MO, Kim HK, Kwon JH (2007) Quality properties of guava (Psidium guajava L.) leaves processed using different methods. Korean J Food Preserv, 14, 605-610

Park BJ, Onjo M (2008) Antioxidant activities and tyrosinase inhibitory effects of guava (Psidium guajava L.) leaf. Korean J Plant Res, 21, 408-412

Heo YJ, Sim KH, Choi HY, Kim SI (2010) Antioxidative activity of crackers made with a guava (Psidium guajava Linn.) leaf extract harvested in Korea. Korean J Food Cookery Sci, 26, 171-179

Jo YH, Ok DL, Lee SC (2009) Antimicrobial characteristics of different parts of guava against food-borne bacteria. J Korean Soc Food Sci Nutr, 38, 1773-1778 crossref(new window)

Hsieh CL, Huang CN, Lin YC, Peng RY (2007) Molecular action mechanism against apoptosis by aqueous extract from guava budding leaves elucidated with human umbilical vein endothelial cell (HUVEC) model. J Agric Food Chem, 55, 8523-8533 crossref(new window)

Kim DO, Jeong SW, Lee CY (2003) Antioxidant capacity of phenolic phytochemical from various cultivars of plums. Food Chem, 81, 321-326 crossref(new window)

Heo HJ, Cho HY, Hong BS, Kim HK, Kim EK, Kim BK, Shin, DH (2001) Protective effect of 4',5- dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against $A\beta$-induced oxidative stress in PC12 cells. Amyloid, 8, 194-201 crossref(new window)

Kwak JH, Choi GN, Park JH, Kim JH, Jeong HR, Jeong CH, Heo HJ (2010) Antioxidant and neuronal cell protective effect of purple sweet potato extract. J Agric Life Sci, 44, 57-66

Chen HY, Yen GC (2007) Antioxidant activity and free radical-scavenging capacity of extracts from guava (Psidium guajava L.) leaves. Food Chem, 101, 686-694 crossref(new window)

Thaipong K, Boonprakob U, Crosby K, Cisneros- Zevallos L, Hawkins Byrne D (2006) Comparison of ABTS, DPPH, FRAP, and ORAC assays fro estimating antioxidant activity from guava fruit extracts. J Food Compost Anal, 19, 669-675 crossref(new window)

Natitanon W, Yotsawimonwat S, Okonogi S (2010) Factors influencing antioxdiant activities and total phenolic contents of guava leaf extract. LWT-Food Sci Technol, 43, 1095-1103 crossref(new window)

Zhao B (2009) Natural antioxidants protect neurons in Alzheimer's disease and Parkinson's disease. Neurochem Res, 34, 630-638 crossref(new window)

Heo HJ, Lee CY (2005) Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells. J Agric Food Chem, 53, 1984-1989 crossref(new window)

Heo HJ, Choi SJ, Choi SG, Shin DH, Lee JM, Lee CY (2008) Effects of banana, orange, and apple on oxidative stress-induced neurotoxicity in PC12 cells. J Food Sci, 73, 28-32 crossref(new window)

Heo HJ, Kim DO, Choi SJ, Shin DH, Lee CY (2004) Apple phenolics protect in vitro oxidative stress-induced neuronal cell death. J Food Sci, 69, 357-360

Wu JW, Hsieh CL, Wang HY, Chen HY (2009) Inhibitory effects of guava (Psidium guajava L.) leaf extracts and its active compounds on the glycation process of protein. Food Chem, 113, 78-84 crossref(new window)