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Expression and Isolation of Limonoid UDP-glucosyltransferase, a Bitterness-reducing Enzyme, in E.coli
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  • Journal title : Korean Journal of Food Preservation
  • Volume 18, Issue 2,  2011, pp.208-211
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2011.18.2.208
 Title & Authors
Expression and Isolation of Limonoid UDP-glucosyltransferase, a Bitterness-reducing Enzyme, in E.coli
K.Cho, So-Mi; Kim, Young-Mee; Kim, Min-Young; Lee, Do-Seung; Kim, Jae-Hoon; Park, Se-Pill; Riu, Key-Zung; Lee, Dong-Sun;
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 Abstract
Limonoids are abundant as bitter taste in citrus fruit and other plants. Interestingly. limonoid UDP-glucosyltransferase (LUGT) effectively ameliorates the bitterness from limonoid. The high level of LUGT expression in Escherichia coli can result in the formation of insoluble aggregates known as inclusion bodies. We isolated the soluble LUGT protein when this inclusion body was renaturated with -cyclidextrin treatment after protein denaturation by urea. Our present results suggest that the isolation of LUGT from inclusion body in cells leads to shed light to characterize the enzyme for food industry purposes.
 Keywords
Citrus grandis Osbeck;expression;limonoid UDP-glucosyltransferase;
 Language
Korean
 Cited by
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