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Effects of Vitamin C on Residual Aflatoxin in Rat Sera Treated with Radiation and Aflatoxin
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  • Journal title : Korean Journal of Food Preservation
  • Volume 18, Issue 3,  2011, pp.374-382
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2011.18.3.374
 Title & Authors
Effects of Vitamin C on Residual Aflatoxin in Rat Sera Treated with Radiation and Aflatoxin
Chung, Do-Young; Kim, Han-Soo; Kang, Jin-Soon;
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Aflatoxin () is a potent hepatotoxic and hepatocarcinogenic mycotoxin in humans. It is also well-known to be accumulated in animal tissues via various metabolic pathways. This study was conducted to determine the effects of vitamin C on the residual in rat sera that were treated with radiation and . Six week-old male Sprague-Dawley rats were randomly divided into five groups: a control group, -treated group, the group treated with and vitamin C, the group treated with X-ray and AFB1, and the group treated with X-ray and with vitamin C. On the first day of the experiment, only one dose of X-rays was exposed to the entire liver at 1,500 cGy. Next, vitamin C was injected at 10 mg/kg body weight via intraperitoneal injection, followed 1 hr later by the administration of 0.4 mg/kg of via intraperitoneal injection. These treatments were then administered every three days over a period of 15 days. On the 16th day of treatments, the animals were sacrificed. The contents of in rat sera were determined via indirect competitive ELISA and HPLC method. In the quantitative analysis of in rat sera via ELISA, ng/mL of was detected in the -treated groups, but the amount more significantly decreased to ng/mL in the groups treated with and vitamin C (p<0.01) than in the -treated groups. The contents of the rat sera of the groups treated with X-ray and did not significantly decreased with the administration of vitamin C. The content of the rat sera that was analyzed via HPLC showed a tendency similar to that of the content that was analyzed via ELISA. With regard to these data, vitamin C was very effective in reducing residue in rat sera.
vitamin C;aflatoxin ;quantitative analysis;ELISA;HPLC;
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