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Identification and Characterization of Wild Yeasts Isolated from Korean Domestic Grape Varieties
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  • Journal title : Korean Journal of Food Preservation
  • Volume 18, Issue 4,  2011, pp.604-611
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2011.18.4.604
 Title & Authors
Identification and Characterization of Wild Yeasts Isolated from Korean Domestic Grape Varieties
Choi, Sang-Hoon; Hong, Young-Ah; Choi, Yoon-Jung; Park, Heui-Dong;
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 Abstract
Several wild yeasts were isolated from Korean grape varieties before and during spontaneous fermentation. Among them, four strains were isolated based on the alcohol content and flavor production in wine after fermentation of apple juice. In this study, the four yeast strains were identified and characterized. PCR-restriction fragment length polymorphism analysis of ITS I-5.8S-ITS II region with restriction endonuclease Hae III and Hinf I resulted in that all the strains showed a typical pattern of Saccharomyces cerevisiae. Pulse field gel electrophoresis showed three different chromosome patterns with a same band between strains SS89 and SS812. When ITS I-5.8S-ITS II sequences of the four strains were compared with one another, they were similar to those of Saccharomyces cerevisiae CBS 4054 type strain. Identity of the sequences was higher than 97% with those of the type strain. Phylogenetic analysis showed based on the sequences showed they were genetically closed to the type strain. The four identified strains were tested in a medium containing 200 ppm potassium metabisulfite, and the MM10 and WW108 inhibition rates resulted at up to 24 h. The four strains were tested at an incubation temperature of . The 30% sugar concentration in the medium (w/v) showed the highest growth in 36 h, especially in the case of SS89, which was close to growth 40. The four strains were tested in an 8% ethanol medium (v/v). Alcohol tolerance was initially kept in the incubation process. The strains began to adapt, however, to the exceeded resistance. The four strains showed the lowest inhibition rate at 24 h.
 Keywords
yeast;PCR-RFLP;PFGE;ITS;phylogenetic tree;grape wine;
 Language
Korean
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 References
1.
Montville TJ, Matthews KR (2005) Food microbiology: an introduction. ASM Press, Washington, DC, USA, p 223-239

2.
Kurtzman CP, Fell JW (1998) The Yeasts, A Taxonomic Study(fourth edition). Elsevier, Amsterdam, The Netherlands, p 358-371

3.
Attfield PV (1997) Stress tolerance. The key to effective strains of industrial baker's yeast. Nat Biotechnol, 15, 1351-1357 crossref(new window)

4.
Pigeau G, Inglis D (2005) Upregulation of ALD3 and GPD1 in Saccharomyces cerevisiae during Icewine fermentation. J Appl Microbiol, 99, 112-125 crossref(new window)

5.
Erasmus DJ, Merwe GK, Vuuren HJJ (2003) Genome-wide expression analyses: metabolic adaptation of Saccharomyces cerevisiae to high sugar stress. FEMS Yeast Res, 2, 375-399

6.
Jimenez-Martí E, Zuzuarregui A, Gomar-Alba M, Gutierrez D, Gil C, del Olmo M (2011) Molecular response of Saccharomyces cerevisiae wine and laboratory strains to high sugar stress conditions. Int J Food Microbiol, 145, 211-220 crossref(new window)

7.
Caridi A, Crucitti P, Ramondino D (1999) Winemaking of must at high osmotic strength by thermotolerant yeast. Biotechnol Lett, 21, 617-620 crossref(new window)

8.
Aguilera F, Peinado RA, Millan C, Ortega JM, Mauricio JC (2006) Relationship between ethanol tolerance, H+-ATPase activity and the lipid composition of the plasma membrane in different wine yeast strains. Int J Food Microbiol, 110, 34-42 crossref(new window)

9.
Zuzuarregui A, Del Olmo M (2004) Expression of stress genes in wine strains with different fermentative behavior. FEMS Yeast Res, 4, 699-710 crossref(new window)

10.
Yook C. Seo MH, Kim DH, Kim JS (2007) Quality improvement of Campbell Early wine by mixing with different fruits. Korean J Food Sci Technol, 39, 390-399.

11.
Guthrie C, Fink RG (1991) Methods in Enzymology: Guide to Yeast Genetics and Molecular Biology. Academic Press, San Diego, California, USA, 194, p 13

12.
Amberg DC, Burke DJ, Strathern JN (2005) Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual. COLD SPRING HARBOR LABORATORY PRESS, Cold Spring Harbor, New York, USA, p 119-120

13.
Hoffman CS, Winston F (1987) A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene, 57, 267-272 crossref(new window)

14.
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungi ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds.) PCR Protocols. A Guide to Methods and Applications. Academic Press, San Diego, California, USA, p 315-322

15.
Sambrook J, Russell DW (2001) Molecular Cloning: A Laboratory Manual(3rd edition). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, Volume 1, Chapter 5, p 5.2-5.17, p 5.55-5.60, p 5.65-5.67, p 5.79-5.82

16.
Clark SM, Lai E, Birren BW, Hood L (1988) A novel instrument for separating large DNA molecules with pulsed homogeneous electric fields. Science, 241, 1203-1205 crossref(new window)

17.
Swaminathan B, Barrett TJ, Hunter SB, Tauxe RV, the CDC PulseNet Task Force (2001) PulseNet: The Molecular Subtyping Network for Foodborne Bacterial Disease Surveillance, United States. Emerg Infect Dis, 7, 382-389 crossref(new window)

18.
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nuclic Acids Symp Ser, 41, 95-98

19.
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol, 24, 1596-1599 crossref(new window)

20.
Torija MJ, Rozes N, Poblet M, Guillamon JM, Mas A (2001) Yeast population dynamics in spontaneous fermentations: comparison between two different wine-producing areas over a period of three years. Antonie Van Leeuwenhoek, 79, 345-352 crossref(new window)

21.
Nisiotou AA, Spiropoulos AE, Nychas GJE (2007) Yeast community structures and dynamics in healthy and Botrytis-affected grape must fermentations. Appl Environ Microbiol, 73, 6705-6713 crossref(new window)

22.
Zott K, Miot-Sertier C, Claisse O, Lonvaud-Funel A, Masneuf-Pomarede I (2008) Dynamics and diversity of non-Saccharomyces yeasts during the early stages in winemaking. Int J Food Microbiol, 125, 197-203 crossref(new window)

23.
Ough CS, Crowell EA (1987) Use of sulphur dioxide in winemaking. J Food Sci, 52, 386-389 crossref(new window)

24.
Abe H, Fujita Y, Takaoka Y, Kurita E, Yano S, Tanaka N, Nakayama K (2009) Ethanol-tolerant Saccharomyces cerevisiae strains isolated under selective conditions by over-expression of a proofreading-deficient DNA polymerase ${\delta}$. J Biosci Bioeng, 108, 199-204 crossref(new window)