JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Resistance and Survival of Cronobacter sakazakii under Environmental Stress of Low Temperature
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Food Preservation
  • Volume 18, Issue 4,  2011, pp.612-619
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2011.18.4.612
 Title & Authors
Resistance and Survival of Cronobacter sakazakii under Environmental Stress of Low Temperature
Kim, Se-Hun; Jang, Sung-Ran; Chung, Hyun-Jung; Bang, Woo-Suk;
  PDF(new window)
 Abstract
Cronobacter sakazakii has been isolated from a wide range of environmental sources and from several foods of animal and plant origin. The objective of this study was to determine the resistance of C. sakazakii (ATCC 12868, ATCC 29004, and ATCC 29544) in cold, cold-freeze thaw, cold-acid, and cold starvation-freeze thaw stress. The number of C. sakazakii decreased to 1 log CFU/mL at (cold storage) for 10 days. When C. sakazakii was cultivated at a low temperature (), the population of C sakazakii ATCC 12868 and 29004 increased to CFU/mL, and the population of C. sakazakii ATCC 29544 increased to CFU/mL. For C. sakazakii ATCC 12868 and 29004, the cold-adapted cells ( 24 hr) decreased by 4 log CFU/mL, and the low-temperature-cultivated cells () decreased by 0.5 log CFU/mL. In this study, low-temperature cultivation enhanced the freeze-thaw cross-resistance due to the metabolic changes in the cells. Cold stress ( 48 hr, cultivation) enhanced the cold-acid cross-resistance. The cold-starved cells in the sterilized 0.1% peptone water enhanced the freeze-thaw cross-resistance with significant differences (p<0.05). Therefore, the increased tolerance of the cold-adapted or low-temperature-cultivated C. sakazakii cells to freeze-thaw, acid, or starvation suggests that such environments should be considered when processing minimally processed foods or foods with extended shelf life.
 Keywords
Cronobacter sakazakii;cold environment;response of cold adaptation;freeze-thaw resistance;
 Language
Korean
 Cited by
 References
1.
Aresni A, Malamou-Ladas E, Koutsia C, Xanthou M, Trikka E (1987) Outbreak of colonization of neonates with Enterobacter sakazakii. J Hosp Infect, 9, 143-150 crossref(new window)

2.
Bar-Oz B, Preminger A, Peleg O, Block C, Arad I (2001) Enterobacter sakazakii infection in the newborn. Acta Paediat, 90, 356-358 crossref(new window)

3.
Ben-Hamouda T, Foulon T, Ben-Cheikh-Masmoudi A, Fendri C, Belhadj O, Gen-Mahrez K (2003) Molecular epidemiology of an outbreak of multiresistant Klebsiella pneumoniae in a Tunisian neonatal ward. J Med Microbiol, 52, 427-433 crossref(new window)

4.
El-Sharoud WM, O'Brien S, Negredo C, Iversen C, Fanning S, Healy B (2009) Characterization of Cronobacter recovered from dried milk and related products. BMC Micrbiol, 9, 24-32 crossref(new window)

5.
Lin LC, Beuchat LR (2007) Survival and growth of Enterobacter sakazakii in infant cereal as affected by composition, reconstitution liquid, and storage temperature. J Food Prot, 70, 1410-1422 crossref(new window)

6.
Beuchat LR, Kim HK, Gurtler JB, Lin LC, Ryu JH, Richards GM (2009) Cronobacter sakazakii in foods and factors affecting its survival, growth, and inactivation. Int J Food Microbiol, 204-213

7.
Miriam F (2007) Enterobacter sakazakii in food and beverages (other than infant formula and milk powder). Int J Food Microbiol, 116, 1-10 crossref(new window)

8.
Iversen C, Forsythe SJ (2003) Risk profile of Enterobacter sakazakii, and emergent pathogen associated with infant milk formula. Trends Food Sci Technol, 14, 443-454 crossref(new window)

9.
Dancer GI, Mah JH, Rhee MS, Hwang IG, Kang DH (2009) Resistance of Enterobacter sakazakii (Cronobacter spp.) to environmental stresses. J Applied Microbiol, 107, 1606-1614 crossref(new window)

10.
Edelson-Mammel SG, Buchanas RL (2004) Thermal inactivation of Enterobacter sakazakii in rehydrated infant formula. J Food Prot, 67, 60-63 crossref(new window)

11.
Lee EJ, Ryu TH, Park JH (2009) Tolerance of Korean Cronobacter spp. (Enterobacter sakazakii) isolated to desiccation. Korean J Food Sci Technol, 41, 681-686

12.
Lee SY, Jin HH (2008) Inhibitory activity of natural antimicrobial compounds alone or in combination with nisin against Enterobacter sakazakii. Letters in Applied Microbiol, 47, 315-321 crossref(new window)

13.
Stock I, Wiedemann B (2002) Natural antibiotic susceptibility of Enterobacter amnigenus, Enterbacter cancerogenus, Enterobacter gergoviae and Enterobacter sakazakii strains. Clin Microbiol Infect, 8, 564-578 crossref(new window)

14.
Abee T, Wouters JA (1999) Microbial stress response in minimal processing. Int J Food Microbiol, 50, 65-91 crossref(new window)

15.
Rosset P, Noel V, Morelli E (2007) Time-temperature profiles of infant milk fomula in hospitals and analysis of Enterobacter sakazakii growth. Food Control, 18, 1412-1418 crossref(new window)

16.
Gurtler JB, Beuchat LR (2007) Survival of Enterobacter sakazakii in powdered infant formula as affected by composition, water activity, and temperature. J Food Prot, 70, 1579-1586 crossref(new window)

17.
Kim HK, Beuchat LR (2005) Survival and growth of Enterobacter sakazakii on fresh-cut fruits and vegetables and in unpasteurized juices as affected by storage temperature. J Food Prot, 68, 2541-2552 crossref(new window)

18.
Leenanon B, Drake MA (2001) Acid stress, starvation, and cold stress affect poststress behavior of Escherichia coli O157:H7 and nonpathogenic Escherichia coli. J Food Prot, 64, 970-974 crossref(new window)

19.
Nazarowec-White M, Farber JM (1997) Incidence, survival, and growth of Enterobacter sakazakii in infant formula. J Food Prot, 60, 226-230 crossref(new window)

20.
Walker SJ (1990) Growth characteristics of food poisoning organism at sub-optimal temperatures. In: Chilled Foods: the revolution in freshness. Zeuthen P, Cheftel JC, Erikson C, Cromley T, Linko R, Paulus K (Editors), Elsevier Applied Science, London, England, p 159-162

21.
Bang WS, Drake MA (2002) Resistance of cold- and starvation-stressed Vibrio vulnificus to heat and freeze-thaw exposure. J Food Prot, 65, 975-980 crossref(new window)

22.
Ferrer M, Chernikova TN, Yakimov MM, Golyshin PN, Timmis KN (2003) Chaperonins govern growth of Escherichia coli at low temperatures. Nat Biotechnol. 21, 1266-1267 crossref(new window)

23.
Berry ED, Foegeding PM (1997) Cold temperature adaptation and growth of microorganisms. J Food Prot, 60, 1583-1594 crossref(new window)

24.
Goldstein J, Pollitt NS, Inouye M (1990) Major cold-shock protein of Escherichia coli. Proc Natl Acad Sci USA, 87, 283-287 crossref(new window)

25.
Willimsky G, Bang H, Fischer G, Marahiel M (1992) Characterization of cspB, a Bacillus subtillis inducible cold shock gene affecting cell viability at low temperatures. J Bacteriol, 174, 6326-6335 crossref(new window)

26.
Gurtler JB, Beuchat LR (2007) Growth of Enterobacter sakazakii in reconstituted infant formula as affected by composition and temperature. J Food Prot, 70, 2095-2103 crossref(new window)

27.
Campbell J, Bang W, Isonhood J, Gerard PD, Drake MA (2004) Effects of salt, acid, and MSG on cold storage survival and subsequent acid tolerance of Escherichia coli O157:H7. Food Microiol, 21, 727-735 crossref(new window)

28.
Elhanafi D, Leenanon B, Bang W, Drake MA (2004) Impact of cold and cold-acid stress on poststress tolerance and virulence factor expression of Escherichia coli O157:H7. J Food Prot, 67, 19-26 crossref(new window)

29.
Farber JM, Forsythe SJ (2008) Enterobacter sakazakii. ASM press, Washington DC, USA, p 15-19

30.
Koutsoumanis KP, Kendall PA, Sofos JN (2004) Modeling the boundaries of growth of Salmonella typhimurium in broth as function of temperature, water activity, and pH. J Food Prot, 67, 53-59 crossref(new window)

31.
De Koning-Ward TF, Robins-Browne RM (1995) Contribution of urease to acid tolerance in Yersinea enterocolitica. Infect Immun, 63, 3790-3795

32.
Kjelleberg S, Hermansson M, Marden P (1987) The transient phase between growth and nongrowth of heterotrophic bacteria, with emphasis on the marine environment. Annu Rev Microbiol, 41, 25-49 crossref(new window)

33.
Martin A, Auger EA, Blum PH, Schultz JE (1989) Genetic basis of starvation survival in nondifferentiating bacteria. Annu Rev Microbiol, 43, 293-316 crossref(new window)

34.
Siegele DA, Kolter R (1992) Life after log. J Bacteriol 174, 345-348 crossref(new window)