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Optimization of 1(3)-Palmitoyl-2-Oleoyl-3(1)-Stearoyl Glycerol Produced via Lipase-catalyzed Esterification Using the Response Surface Methodology
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  • Journal title : Korean Journal of Food Preservation
  • Volume 18, Issue 5,  2011, pp.721-728
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2011.18.5.721
 Title & Authors
Optimization of 1(3)-Palmitoyl-2-Oleoyl-3(1)-Stearoyl Glycerol Produced via Lipase-catalyzed Esterification Using the Response Surface Methodology
Hwang, Yun-Ik; Shin, Jung-Ah; Lee, Jeung-Hee; Hong, Soon-Taek; Lee, Ki-Teak;
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 Abstract
1(3)-palmitoyl-2-oleoyl-3(1)-stearoyl-(POS)-glycerol-enriched reaction products were synthesized from camellia oil, palmitic ethyl ester, and stearic ethyl ester via lipase-catalyzed interesterification. Response surface methodology (RSM) was employed to optimize the production of the POS-enriched reaction product (Y1, %) and the stearicand palmitic-acid contents at the sn-2 position due to acyl migration (Y2, %). The reaction factors were the enzyme amount (X1, 2-6%), reaction time (X2, 60-360 min), and substrate molar ratio of camellia oil to palmitic ethyl ester and stearic ethyl ester (X3, 1-3 mol). The predictive models for Y1 and Y2 were adequate and reproducible as no lack of fit was signified (0.128 and 0.237) and as there were satisfactory levels of R2 (0.968 and 0.990, respectively). The optimal conditions for the reaction product for maximizing Y1 while minimizing Y2 were predicted at the reaction combination of 5.86% enzyme amount, 60 min reaction time, and 1:3 substrate molar ratio (3 moles of palmitic ethyl ester and 3 moles of stearic ethyl ester). Actual reaction was performed under the same conditions as above, and the resulting product contained 20.19% TAG-P/O/S and 12.71% saturated fatty acids at the sn-2 position.
 Keywords
camellia oil;lipase-catalyzed interesterification;cocoa butter equivalent;1(3)-palmitoyl-2-oleoyl-3(1)-stearoyl (POS) glycerol;acyl migration;response surface methodology;
 Language
Korean
 Cited by
 References
1.
Wang HX, Wu H, Ho CT, Weng XC (2006) Cocoa butter equivalent from enzymatic interesterification of tea seed oil and fatty acid methyl esters. Food Chem, 97, 661-665 crossref(new window)

2.
Buchgraber M, Ulberth F, Anklam E (2004) Cluster analysis for the systematic grouping of genuine cocoa butter and cocoa butter equivalent samples based on triglyceride patterns. J Agric Food Chem, 52, 3855-3860 crossref(new window)

3.
Fang T, Zhang H (1997) Rheological behavior of cocoa dispersions with cocoa butter replacers. J Texture Stud, 28, 11-26 crossref(new window)

4.
Undurraga D, Markovits A, Erazo S (2001) Cocoa butter equivalent through enzymic interesterification of palm oil midfraction. Process Biochem, 36, 933-939 crossref(new window)

5.
Kristensen JB, Xu X, Mu H (2005) Diacylglycerol synthesis by enzymatic glycerolysis screening of commercially available lipases. J Am Oil Chem Soc, 82, 329-334 crossref(new window)

6.
Kim B.H. and Akoh CC (2005) Modeling of lipase- catalyzes acidolysis of sesame oil and caprylic acid by response surface methodology: optimization of reaction conditions by considering both acyl incorporation and migration. J Agric Food Chem, 53, 8033-8037 crossref(new window)

7.
Lee KT, Akoh CC. (1996) Immobilized lipase-catalyzed production of structured lipids with eicosapentaenoic acid at specific positions. J Am Oil Chem Soc, 73, 611-615 crossref(new window)

8.
Simoneau C, Hannaert P, Anklam E (1999) Detection and quantification of cocoa butter equivalents in chocolate model systems: analysis of triglyceride profiles by high resolution GC. Food Chem, 65, 111-116 crossref(new window)

9.
Cho EJ, Lee JH, Lee KT (2004) Optimization of enzymatic synthesis condition of structured lipids by response surface methodology. Korean J Food Sci Technol, 36, 531-536

10.
Park RK, Choi SW, Lee KT (2004) Optimization for the production of mono- and di-acylglycerols from corn oil by enzymic glycerolysis using response surface methodology. Korean J Food Sci Technol, 36, 717-722