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Antimutagenicity and Immuno Activity of Extracts from Epimedium koreanum Nakai Containing Different Icariin Quantity
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  • Journal title : Korean Journal of Food Preservation
  • Volume 18, Issue 6,  2011, pp.938-945
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2011.18.6.938
 Title & Authors
Antimutagenicity and Immuno Activity of Extracts from Epimedium koreanum Nakai Containing Different Icariin Quantity
Park, Myoung-Su; Kim, Seo-Jin; Forghani, Fereidoun; Rahman, S.M.E.; Eo, Ji-Hyun; Eun, Jong-Bang; Oh, Deog-Hwan;
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 Abstract
Epimedium koreanum Nakai is a wild herb commonly consumed in South Korea due to its beneficial health effects. In the present study, the antimutagenic and immunoactivities of extracts from E. koreanum Nakai containing different icariin quantities were investigated for food use. In the Ames test, both the water and ethanol extracts were found not to have a mutagenic effect on Salmonella typhimurium TA98 and TA100, respectively. The E. koreanum Nakai extracts showed over 80 and 90% antimutagenic effects on benzo()pyrene (B(a)P) in S. typhimurium TA98 and TA100, respectively. Moreover, all the extracts showed over 70% antimutagenicity on S. typhimurium TA98 and TA100 against 4-nitroquinoline-1-oxide (4NQO). The E. koreanum Nakai extract with ethanol showed strong antimutagenic activity, higher than that of the water extract and the sequenced KE9412, KE9408, and KE9405. In the immunomodulating activity test, the effect of E. koreanum Nakai on the B (Rhamos) and T (Jukat) cells were investigated. The immunoactivity results showed that the growth and viability of the B and T cells increased and were activated more in KE9405 (1.8 times), KE9408 (1.6 times), and KE9412 (1.32 times) in the water extracts, and least in KE9412 (1.74 times), KE9408 (1.52 times), and KE9405 (1.4 times) in the ethanol extracts. In the case of both the water and ethanol extracts () from E. koreanum Nakai, the highest cell number of the human B (Rhamos) and T (Jukat) cells was observed on day 4 in KE9405 and KE9412, and on day 5 in KE9408. Based on the obtained results, the development of E. koreanum Nakai as a food material is recommended.
 Keywords
Epimedium koreanum Nakai;icariin;Ames test;MTT assay;
 Language
Korean
 Cited by
1.
Genetic toxicity of Epimedium koreanum Nakai, Journal of Ethnopharmacology, 2017, 198, 87  crossref(new windwow)
 References
1.
Noh JH, Kim YJ, Kim SW, Lee JH, Lee HY (2003a) Comparison of biological activities of Epimedium koreanum produced in korea and china. Kor J Med Crop Sci, 11, 195-200

2.
Noh JH, Kim YJ, Choi KJ, Kim SW, Kim SK, Kim JH (2003b) Characteristics of seedling and rhizoe propagation in Epimedium koreanum Nakai. Kor J Med Crop Sci, 11, 155-160

3.
Kang SS, Shin KH, Chung SG, Cho EH (1998) Flavonoids from Epimedium koreanum. Kor J Pharmacogn, 19, 93-98

4.
Xu SC, Xu BJ, Wang MT (1987) Isolation and identification of icariin and icariside I. Chin Pharm Bull, 22, 129-132

5.
Fukai T, Nomura T (1988) Seven prenylated flavonol glycosides from two epimedium species. Phytochem, 27, 259-266 crossref(new window)

6.
Mizuno M, Hanioka S, Suzuki N, Iinuma M, Tanaka T, Liu XS, Min ZD (1987) Flavonol glycosides from epimedium sagittatum. Phytochem, 226, 861-863

7.
Sun P, Zhao Ye W, Pei J, Wang Z, Chen Y, Ogihara Y, Takeda T (1995) Studies on the constituents of Epimedium koreanum. Chem Pharm Bull, 43, 703-704 crossref(new window)

8.
Kang SS, Shin KH, Chung SG, Cho EH (1988) Flavonoids from Epimedium koreanum. Kor J Pharmacogn, 21, 93-96

9.
Lee MK, Choi YJ, Sung SH, Shin DI, Kim JW, Kim YC (1995) Antihepatotoxic activity of icariin, a major constituent of Epimedium koreanum. Planta Med, 61, 523-526 crossref(new window)

10.
Xu SC, Wang ZX, Wu LJ, Wang NB, Chen YJ (1982) Isolation and identification do icariin and epimedoside A. Chin Tard Herb Drug, 13 9-15

11.
Park BJ, Choi SY, Chang KJ, Cho DH, Heo K, Park CH (1997) Vegetation and environment in nature habit of Asterscaber and Epimedium koreanum around Chunchon. Kor J Crio Sci, 6, 51-56

12.
Park KY, Choi BR, Yi ES, Kim SJK, Park CH (1998) Habit environment of Epimedium koreanum Nakai. Kor J Nutr, 27, 311-322

13.
Shin, KH, Lim SS, Ahn Sd, Kim SK, Park KY (1996) Difference in components of Epimedium koreanum in compliance with seasons and places of collection. Kor J Med Crop Sci, 4, 321-328

14.
Gies J (1996) Antioxidants tools for preventing liquid oxidation. Food Tech, 50, 73-76

15.
Kang CS, Choi BR, Park KY, Ahn SD (1997) Establishment of growth environment and mass propagation system development for Epimedium Koreanum. Kor J Pharmacogn, 30, 378-381

16.
Ames BN, Maron DM (1983) Revised methods for the Salmonella typhyimurium mutagenicity test. Mutation Res, 113, 173-215 crossref(new window)

17.
Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbortt BJ, Mayo JG, Shoemarker RH, Boyd MR (1988) Feasibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Res, 48, 589-601

18.
Carmichael J, De Graff WG, Gazder AF, Minna JD, Mitchell JB (1987) Evaluation of the tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res, 47, 936-942

19.
Lee JH (1989) Studies on the demutagenic effect of the edible mountain herb juices. MS Thesis. Kangwon National University, Gangwon, Korea.

20.
Ito Y, Maeda S, Sugiyama T (1986) Suppression of 7,12-dimethylbenzo(a)anthracene-induced chromosome aberrations in rat bone marrow cells by vegetable juices. Mutat Res, 172, 55-60 crossref(new window)

21.
Han KS, Ham SS, Jeong EH, Lee HK (1992) Antimutagenic effects of the edible mountain herb juices against Trp-P-1 and 2-AF. Kor J Food Hyg, 7, 161-168

22.
Tognolini M, Barrocelli E, Ballabeni V, Bruni R, Bianchi A, Chivarini M, Impicciatore M (2006) Comparative screening of plant essential oils. Life Sci, 78, 1419-1432 crossref(new window)

23.
Gardeli C, Vassilikil P, Athanasios M, Kiboutis T, Komaitis M (2008) Essential oil composition of Epimedium koreanum Nakai and evaluation of antioxidant capacity of methanolic extracts. Food Chem, 107, 1120-1130 crossref(new window)

24.
Grabmann J, Hipeli S, Spitzenberger R, Elstner EF (2005) The icariin from the oil of Epimedium koreanum Nakai in concert with tocopherol and carotene effectively prevents oxidation of LDL. Phytomedicine, 12, 416-423 crossref(new window)

25.
Ng TB, Liu F, Lu Y, Cheng CHK, Wang Z (2003) Antioxidant activity of compounds from the medicinal herb Epimedium koreanum Nakai. Comp Biochem Phys, 136, 109-115

26.
Singh HP, Mital S, Kaur S, Batish DR, Kohli RK (2009) Chemical composition and antioxidant activity of essential oil from residues of Epimedium koreanum Nakai. Food Chem, 114, 642-645 crossref(new window)

27.
Tepe B, Sihoglu-Tepe A, Daferera D, Polissiou M, Sokmen A (2007) Chemical composition and antioxidant activity of the essential oil of Epimedium koreanum Nakai. Food Chem, 103, 766-770. crossref(new window)

28.
Fragoso V, Fett-Neto AG (2008) Antioxidant and antimutagenic properties of the icariin of Epimedium koreanum Nakai. Toxicol, 22, 559-566

29.
Marin-Martinez R, Veloz-Gare R, Veloz-Rodriguez R, Guzman-Maldonado SH, Loarca-Pina G, Cardador-Martinez A, Guevara-Olvera L, Miranda-Lopez R, Torres-Pacheco I, Perez CPE, Herrera-Hernandez G, Villasenor-Ortega F, Gonzalex-Chavira M, Guevara-Gonzalez RG (2009) Antimutagenic and antioxidant activities of Epimedium koreanum Nakai recovered from tannery wastewaters. Bioresource Technol, 100, 434-439 crossref(new window)

30.
Rocha-Guzm NE, Herzog A, Gonzalez-Laredo RF, Ibarra-Perez FJ, Zambra-Galv G, Gallegos-Infante JA (2007) Antioxidant and antimutagenic acitivity of phenolic compounds in 3 different colour group of common Epimedium koreanum Nakai. Food Chem, 103, 521-527 crossref(new window)

31.
Shon MY, Choil SD, Kahng GG, Nam SH, Sung NJ (2004) Antimutagenic, antioxidant and free radical scavenging activity of ethylacetate extracts from Epimedium koreanum Nakai. Food Chem Toxicol, 42, 659-666 crossref(new window)

32.
Kim MJ, Kim JS, Kang WH, Jeong DM (2002) Effect on antimutagenic and cancer cell growth inhibition of Lxeris dentata Nakai. Kor J Med Crop Sci, 10, 139-143

33.
Hwang HS, Ham SS (1999) Antimutagenic and cytotoxic effects of aster acaber root ethanol extract. Kor J Food Sci Technol, 31, 1065-1070

34.
Ahn BY, Lee KS, Maeng IK, Song GS, Choi DS (1998) Bioantimutagenic effects of water extract from Rehmannia glutinosa Liboschitz in SOS chromotest. Kor J Food Sci Technol, 30, 439-445

35.
Park MH, Kang SM, Jung HY, Hong SG (2003) Protecting effects of vitamin E against immobilization stress-induced oxidative damage in rat brain. Kor Nutr, 36, 570-576