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Survey and method validation of simultaneous quantitative analysis of T-2 and HT-2 toxins in cereals
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  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 4,  2015, pp.559-566
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.4.559
 Title & Authors
Survey and method validation of simultaneous quantitative analysis of T-2 and HT-2 toxins in cereals
Paek, Ockjin; Kang, Teabeom;
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 Abstract
The aim of this study was to develop an analytical method for determination of T-2 toxin and HT-2 toxin level in cereals and to survey their levels using LC-MS/MS. The T-2 and HT-2 toxins were simultaneously analyzed by electrospray ionization with a positive ion mode and multiple reaction monitoring (MRM) after filteration and immuno-affinity column clean-up. A matrix-matched standard calibration used for quantification and recoveries of T-2 and HT-3 toxins were in the range of and , respectively. Limits of detection and quantification of T-2 and HT-2 toxins were estimated to be 0.5 and , respectively. Each repeatability (RSRr) of T-2 and HT-2 toxins was determined to be 0.9~6.0%, and 4.9~6.1%, respectively. Total 115 samples cereals were collected from 9 types of cereals for analysis. The positive percentages of T-2 and HT-2 toxins obtained from collected samples were found to be 72% and 80%, respectively. The contamination level of T-2 toxin and HT-2 toxin in cereals were , and , respectively. Therefore, this study suggests that the developed method could be an useful analytical method to determine the T-2 and HT-2 toxin level in cereals and the present data could be used as a reference to estimate the risk assessment.
 Keywords
T-2 toxin;HT-2 toxin;LC-MS/MS;cereals;survey;
 Language
Korean
 Cited by
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