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Survey and method validation of simultaneous quantitative analysis of T-2 and HT-2 toxins in cereals
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  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 4,  2015, pp.559-566
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.4.559
 Title & Authors
Survey and method validation of simultaneous quantitative analysis of T-2 and HT-2 toxins in cereals
Paek, Ockjin; Kang, Teabeom;
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The aim of this study was to develop an analytical method for determination of T-2 toxin and HT-2 toxin level in cereals and to survey their levels using LC-MS/MS. The T-2 and HT-2 toxins were simultaneously analyzed by electrospray ionization with a positive ion mode and multiple reaction monitoring (MRM) after filteration and immuno-affinity column clean-up. A matrix-matched standard calibration used for quantification and recoveries of T-2 and HT-3 toxins were in the range of and , respectively. Limits of detection and quantification of T-2 and HT-2 toxins were estimated to be 0.5 and , respectively. Each repeatability (RSRr) of T-2 and HT-2 toxins was determined to be 0.9~6.0%, and 4.9~6.1%, respectively. Total 115 samples cereals were collected from 9 types of cereals for analysis. The positive percentages of T-2 and HT-2 toxins obtained from collected samples were found to be 72% and 80%, respectively. The contamination level of T-2 toxin and HT-2 toxin in cereals were , and , respectively. Therefore, this study suggests that the developed method could be an useful analytical method to determine the T-2 and HT-2 toxin level in cereals and the present data could be used as a reference to estimate the risk assessment.
T-2 toxin;HT-2 toxin;LC-MS/MS;cereals;survey;
 Cited by
Bailly JD, Tabuc C, Querin A, Guerr P (2005) Production and stability of patulin, ochratoxin A, citrinin and cyclopiazonic acid on dry cured ham. J Food Protection, 68, 1516-1520

Battacone G, Nudda A, Palomba M, Pascale M, Nicolussi P, Pulina G (2005) Transfer of aflatoxin B1 from feed to milk and curd and whey in dairy sheep fed artificially contaminated concentrates. J Dairy Sci, 88, 3063-3069 crossref(new window)

OK HY, Sim JH, Park KH, Chun HS (2013) Climate change and control strategies to support prevention of aflatoxins contamination. Safe Food, 8, 36-45

Jaimea J, Fente CA, Franco CM, Cepeda A, Vazquez BI (2004) A survey of the fungal contamination and presence of ochratoxin A and zearalenon e on Spanish feed and raw materials. J Sci Food Agric, 87, 832-840

Degola F, Berni E, Dall'Asta C, Spotti E, Marchelli R, Ferrero I, Restivo EM (2006) A multiplex RT-PCR approach to detect aflatoxigenic strains of Aspergillus flavus. J Appl Microbiol, 103, 409-417

Chen D, Cao X, Tao Y, Wu Q, Pan Y, Huang L, Wang X, Wang Y, Peng D, Liu Z, Yuan Z (2012) Development of a sensitive and robust liquid chromatography coupled with tandem mass spectrometry and a pressurized liquid extraction for the determination of aflatoxins and ochratoxin A in animal derived foods. J Chromatography A, 1253, 110-119 crossref(new window)

Korea Centers for Disease Control & Prevention (2008) Korea Health Statistics 2008 : Korea National Health and Nutrition Examination Survey. Ministry of Health and Welfare, Seoul, Korea

Lee S, Kim M, Oh S, Chun HS (2012) Trends in researches of fusarium mycotoxins of T-2 toxins and HT-2 toxins in domestic and foreign countries. J Fd Hyg Safety, 27, 1-17 crossref(new window)

EU (2006) Commission Regulation(EC) N0 401/2006. The methods of sampling and analysis for the official control of the levels of mycotoixns in foodstuffs, L70, p 12-34

Lauren DR, Agnew MP (1991) Multitoxin screening method for Fusarium mycotoxins in grains. J Agrc Food Chem, 39, 502-507 crossref(new window)

EU (2003) Directorate General Health and Consumer Protection : Collection of occurrence data of Fusarium toxins in food and assessment of dietary intake by the population of EU Member States. Final Report SCOOP Task 3. 2. 10