Advanced SearchSearch Tips
Potent whitening effects of rutin metabolites
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 4,  2015, pp.607-612
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.4.607
 Title & Authors
Potent whitening effects of rutin metabolites
Kim, Ji Hye; Kang, Nam Joo;
  PDF(new window)
The aim of this research was to investigate the whitening effects of rutin and rutin metabolites including 3,4-dihydroxyphenyl acetic acid (DHPAA), 3-hydroxyphenyl acetic acid (HPAA), 3,4-dihydroxytolene (DHT) and homovanillic acid (HVA). The potent whitening effect of rutin and rutin metabolites were determined by mushroom tyrosinase inhibition assay and expressed as the half maximal inhibitory concentration () against tyrosinase activity in vitro. The HVA showed the highest inhibitory effect () of tyrosinase activity, followed by DHPAA (), HPAA (), rutin (), and DHT (), respectively. To evaluate cell cytotoxicity, MTT assay was performed with JB6 P+ mouse epidermal cells and expressed as a relative percentage of untreated control. The results showed that rutin and rutin metabolites had no cytotoxic effects on JB6 P+ cells up to except for DHT (up to ). These results suggests that rutin metabolites may be utilized as a potential tyrosinase inhibitors and the whitening agents for the future.
rutin;rutin metabolites;tyrosinase activity;melanin;whitening effect;
 Cited by
Agar N, Young AR (2005) Melanogenesis : a photoprotective response to DNA damage? Mutation Res, 1, 121-132

Ando H, Kondoh H, Ichihashi M, Hearing VJ (2007) Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase. J Invest Dermatol, 127, 751-761 crossref(new window)

Solano F, Briganti S, Picardo M, Ghanem G (2006) Hypopigmenting agents : an updated review on biological, chemical and clinical aspects. Pigment Cell Res, 19, 550-571 crossref(new window)

Kreft I, Fabjan N, Yasumoto K (2006) Rutin content in buckwheat (Fagopyrum esculentum Moench) food materials and products. Food Chem, 98, 508-512 crossref(new window)

Li L, Henry GE, Seeram NP (2009) Identification and bioactivities of resveratrol oligomers and flavonoids from Carex folliculata seeds. J Agric Food Chem, 26, 7282-7287

Maria A, Valentina B (2009) Rutin content in plant products. J Univ Chem Technol Metallurgy, 44, 2

Jaganath IB, Mullen W, Lean ME, Edwards CA, Crozier A (2009) In vitro catabolism of rutin by human fecal bacteria and the antioxidant capacity of its catabolites. Free Radic Biol Med, 47, 1180-1189 crossref(new window)

Pashikanti S, de Alba DR, Boissonneault GA, Cervantes-Laurean D (2010) Rutin metabolites : novel inhibitors of nonoxidative advanced glycation end products. Free Radic Biol Med, 48, 656-663 crossref(new window)

Chua LS (2013) A review on plant-based rutin extraction methods and its pharmacological activities. J Ethnopharmacol, 150, 805-817 crossref(new window)

Hwang E1, Park SY, Lee HJ, Sun ZW, Lee TY, Song HG, Shin HS, Yi TH (2014) Vigna angularis water extracts protect against ultraviolet b-exposed skin aging in vitro and in vivo. J Med Food, 17, 1339-1349 crossref(new window)

Stipcevic T, Piljac J, Vanden Berghe D (2006) Effect of different flavonoids on collagen synthesis in human fibroblasts. Plant Foods Hum Nutr, 61, 29-34

Si YX1, Yin SJ, Oh S, Wang ZJ, Ye S, Yan L, Yang JM, Park YD, Lee J, Qian GY (2012) An integrated study of tyrosinase inhibition by rutin : progress using a computational simulation. J Biomol Struct Dyn, 29, 999-1012 crossref(new window)

Xie LP1, Chen QX, Huang H, Wang HZ, Zhang RQ (2003) Inhibitory effects of some flavonoids on the activity of mushroom tyrosinase. Biochem (Mosc), 68, 487-491 crossref(new window)

An SM, Kim HJ, Kim JE, Boo YC (2008) Flavonoids, taxifolin and luteolin attenuate cellular melanogenesis despite increasing tyrosinase protein levels. Phytother Res, 22, 1200-1207 crossref(new window)

Pavlica S, Gebhardt R (2010) Protective effects of flavonoids and two metabolites against oxidative stress in neuronal PC12 cells. Life Sci, 86, 79-86 crossref(new window)

Maeda K, Fukuda M, Fujii T (1991) In vitro effectiveness of several whitening cosmetic components in human melanocytes. J Soc Cosmet Chem, 4, 361-368

Chang TS (2012) Natural melanogenesis inhibitors acting through the down-regulation of tyrosinase activity, Materials, 5, 1661-1685 crossref(new window)

Leyden JJ, Shergill B, Micali G (201) Natural options for the management of hyperpigmentation. J Eur Acad Dermatol Venereol, 25, 1140-1145 crossref(new window)

Saito M (2009) Inhibitory effect of quercetin isolated rose hip (Rosa canina L.) against melanogenesis by mouse melanoma cells. Biosci Biotechnol Biochem, 73, 1989-1993 crossref(new window)

An SM, Koh JS, Boo YC (2010) p-coumaric acid not only inhibits human tyrosinase activity in vitro but also melanogenesis in cells exposed to UVB. Phytother Res, 24, 1175-1180

Briganti S, Camera E, Picardo M (2003) Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res, 16, 101-110 crossref(new window)

Chen WC, Tseng TS, Hsiao NW, Lin YL, Wen ZH, Tsai CC, Lee YC, Lin HH, Tsai KC (2015) Discovery of highly potent tyrosinase inhibitor, T1, with significant anti-melanogenesis ability by zebrafish in vivo assay and computational molecular modeling. Sci Rep, 5, 7995 crossref(new window)

Lerch K (1978) Amino acid sequence of tyrosinase from Neurospora crassa. Proc Natl Acad Sci USA, 75, 3635-3639 crossref(new window)

Kwon BS, Haq AK, Pomerantz SH, Halaban R (1987) Isolation and sequence of a cDNA clone for human tyrosinase that maps at the mouse c-albino locus. Proc Natl Acad Sci USA, 84, 7473-7477 crossref(new window)

Klabunde T, Eicken C, Sacchettini JC, Krebs B (1998) Crystal structure of a plant catechol oxidase containing a dicopper center. Nat Struct Biol, 5, 1084-1090 crossref(new window)

Galindo JD, Martinez-Liarte JH, Lopez-Ballester JA, Penafiel R, Solano F, Lozano JA (1987) The effect of polyamines on tyrosinase activity. Biochem Int, 15, 1151-1158

Jacobsohn GM, Jacobsohn MK (1992) Incorporation and binding of estrogens into melanin : comparison of mushroom and mammalian tyrosinases. Biochim Biophys Acta, 1116, 173-182 crossref(new window)

Funayama M, Arakawa H, Yamamoto R, Nishino T, Shin T, Murao S (1995) Effects of ${\alpha}$- and ${\beta}$-arbutin on activity of tyrosinases from mushroom and mouse melanoma. Biosci Biotech Biochem, 59, 143-144 crossref(new window)

Pastore S, Potapovich A, Kostyuk V, Mariani V, Lulli D, De Luca C, Korkina L (2009) Plant polyphenols effectively protect HaCaT cells from ultraviolet C-triggered necrosis and suppress inflammatory chemokine expression. Ann N Y Acad Sci, 1171, 305-313 crossref(new window)

Nagao T, Abe F, Okabe H (2001) Antiproliferative constituents in the plants 7. leaves of Clerodendron bungei and leaves and bark of C. trichotomum. Biol Pharm Bull, 24, 1338-1341 crossref(new window)

Morita K, Arimochi H, Ohnishi Y (2003) In vitro cytotoxicity of 4-methylcatechol in murine tumor cells : induction of apoptotic cell death by extracellular pro-oxidant action. J Pharmacol Exp Ther, 306, 317-323 crossref(new window)