JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effect of garlic (Allium sativum L.) stems on inflammatory cytokines, iNOS and COX-2 expressions in Raw 264.7 cells induced by lipopolysaccharide
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 4,  2015, pp.613-621
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.4.613
 Title & Authors
Effect of garlic (Allium sativum L.) stems on inflammatory cytokines, iNOS and COX-2 expressions in Raw 264.7 cells induced by lipopolysaccharide
Cho, Yong Hun; Kim, Hyeon Jeong; Kim, Dong In; Jang, Jae Yoon; Kwak, Jae Hoon; Shin, Yu Hyeon; Cho, Yeon Gje; An, Bong Jeon;
  PDF(new window)
 Abstract
In this study, the anti-oxidant and anti-inflammatory activities of water extract (ASSW) and 70% ethanol extract (ASSE) of Allium sativum L. stems were investigated using Raw 264.7 cells induced by lipopolysaccharide (LPS). ABTS radical scavenging activities of ASSW and ASSE at concentration were 96.9% and 97.8%, respectively. In order to investigate the potential anti-inflammatory effects of ASSW and ASSE, nitric oxide (NO), pro-inflammatory cytokines, interleukin-6 (IL-6), and tumor necrosis factor including (TNF-), interleukin- (IL-), and prostaglandin-E2 (PGE2) were measured. ASSW and ASSE at concentration showed inhibitory effects against NO production by 18% and 23%, respectively. Production of IL- and IL-6 after treatment with ASSW and ASSE at decreased by approximately 28% and 15% for ASSW and 17% and 12% for ASSE, respectively. In addition, production of TNF- after treatment of of ASSW and ASSE decreased by 24% and 23%, respectively. In addition, the treatment of of ASSW and ASSE showed inhibitory expressions against PGE2 by 45.47% and 33.87%, respectively. These results suggested that ASSE showed greater inhibitory activity than that of the ASSW by the suppression of inflammatory mediators, including NO, IL-6, TNF- and PGE2 production, and the expressions of iNOS and COX-2 in macrophages. In conclusion, ASSW and ASSE may have some ancillary effects on inflammatory factors as potential anti-inflammatory agents.
 Keywords
anti-oxidant;iNOS;COX-2;cytokine;Allium sativum;
 Language
Korean
 Cited by
 References
1.
Haddad JJ (2002) Antioxidant and prooxidant mechanisms in the regulation of redox (y)-sensitive transcription factor. Cell Signal, 14, 879-897 crossref(new window)

2.
Beckman KB, Ames BN (1998) The free radical theory of aging matures. Physiol Rev, 78, 547-581

3.
Balaban RS, Nemoto S, Finkel T (2005) Mitochondria, oxidants, and aging. Cell, 120, 483-495 crossref(new window)

4.
Sohal RS, Weindruch R (1996) Oxidative stress, caloric restriction, and aging. Science, 273, 59-63 crossref(new window)

5.
Davies KJ (2000) Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. IUBMB Life, 50, 279-289 crossref(new window)

6.
Medzhitov R (2008) Origin and physiological roles of inflammation. Nature, 454, 428-435 crossref(new window)

7.
Guha M, Mackman N (2001) LPS induction of gene expression in human monocytes. Cell Signal, 13, 85-94 crossref(new window)

8.
Coussens LM, Werb Z (2002) Inflammation and cancer. Nature, 420, 860-867 crossref(new window)

9.
Posadas I, Terencio MC, Guillén I, Ferrándiz ML, Coloma J, Payá M, Alcaraz MJ (2000) Co-regulation between cyclo-oxygenase-2 and inducible nitric oxide synthase expression in the time-course of murine inflammation. N-S Arc Pharmacol, 361, 98-106 crossref(new window)

10.
Cencioni C, Spallotta F, Martelli F, Valente S, Mai A, Zeiher AM, Gaetano C (2013) Oxidative stress and epigenetic regulation in ageing and age-related diseases. Int J Mol Sci, 14, 17643-17663 crossref(new window)

11.
Kundu JK, Surh YJ (2008) Inflammation: gearing the journey to cancer. Mutat Res-Rev Mutat, 659, 15-30 crossref(new window)

12.
Lee TB (1979) Illustrated flora of Korea, Hangmunsa, Seoul, Korea, p 203

13.
Jo JS, Hwang SY (1990) Gijeunyungusa, Seoul, Korea, p 154-155

14.
Bozin B, Mimica-Dukic N, Samojlik I, Goran A, Igic R (2008) Phenolics as antioxidants in garlic (Allium sativum L., alliaceae). J Agric Food Chem, 111, 925-929 crossref(new window)

15.
Lee MK, Park JS, Na HS (2005) Proximate compositions of green garlic powder and microbiological properties of bread with green garlic. Korean J Food Preserv, 12, 95-100

16.
Folin O, Denis W (1912) On phosphotungasticphosphomolybetic compounds as color regents. J Biol Chem, 12, 239-249

17.
Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell HB (1987) Evaluation of a tetrazolium based semiautomated colorimetric assay : assessment of chemosensitivity testing. Cancer Res, 47, 936-942

18.
Ding AH, Nathan CF, Stueher DJ (1988) Release of reactive nitrogen intermediates and macrophages. Comparison of activation cytokines and evidence for independent production. J Immunol, 141, 2407-2412

19.
Duthie G, Crozier A (2000) Plant-derived phenolic antioxidants. Curr Opin Clin Nutr Metab Care, 3, 447-451 crossref(new window)

20.
Ferreres F, Gomes D, Valentao P, Goncalves R, Pio R, Chagas EA, Seabra RM, Andrade PB (2009) Improved loquat (Eriobotrya japonica Lindl.) cultivars : variation of phenolics and antioxidative potential. Food Chem, 114, 1019-1027 crossref(new window)

21.
Kromhout D (1987) Essential micronutrients in relation to carcinogenesis. Am J Clin Nutr, 45, 1361-1467

22.
Park SJ, Park DH, Kim SS, Gon J, Lee HY (2009) Chemical compositions of fermented Codonopsis lanceolata. J Food Sci Nutr, 38, 396-400

23.
Moon JS, Kim SJ, Par YM, Hwang IS, Kim EY, Park JW, Park IB, Kim SW, Kang WG, Park YK, Jung ST (2004) Antimicrobial effect of methanol extracts from some medicinal herbs and the content of phenolic compounds. Korean J Food Preserv, 11, 207-213

24.
Molyneux P (2004) The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol, 26, 211-219

25.
Lu Y, Foo LY (2000) Antioxidant and radical scavenging activities of polyphenols from apple pomace. J Agric Food Chem, 68, 81-85 crossref(new window)

26.
Rice-Evans CA, Miller NJ, Paganga G (1996) Structureantioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med, 20, 933-956 crossref(new window)

27.
Rice-Evans CA, Miller NJ, Bolwell GP, Bramley PM, Pridham JB (1995) The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res, 22, 375-383 crossref(new window)

28.
Miller NJ, Sampson J, Candeias LP, Bramley PM, Rice-Evans CA (1996) Antioxidant activities of carotenes and xanthophylls. FEBS Lett, 384, 240-242 crossref(new window)

29.
Weisz A, Cicatiello L, Esumi H (1996) Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferongamma, bacterial lipopolysaccharide and NG-monomethyl-L-arginine. Biochem J, 316, 209-215 crossref(new window)

30.
An SM, Kim HG, Choi EJ, Hwang HH, Lee ES, Baek JH, Boo YC, Koh JS (2014) Screening for antiinflammatory activities in extracts from Korean herb medicines. J Soc Cosmet Scientists Korea, 40, 95-108 crossref(new window)

31.
Kang CH, Choi YH, Choi IW, Lee JD, Kim GY (2011) Inhibition of lipopolysaccharide-induced iNOS, COX-2, and TNF-${\alpha}$ expression by aqueous extract of Orixa japonica in Raw 264.7 cells via suppression of NF-${\kappa}B$ activity. Trop J Pharm Res, 10, 161-168

32.
Shon MS, Song JH, Kim JS, Jang HD, Kim GN (2013) Anti-oxidant activity of oil extracted from Korean red ginseng and its moisturizing function. Korean J Aesthet Cosmetol, 11, 489-494

33.
Lee HJ, Sim BY, Bak JW, Kim DH (2014) Effect of Gami-sopungsan on inflammation and DNCB-induced dermatitis in NC/Nga in mice. Korean J Orient Physiol Pathol, 28, 146-153

34.
Kuo, Li C, Chi CW, Liu TY (2004) The antiinflammatory potential of berberine in vitro and in vivo. Cancer letters, 203, 127-137 crossref(new window)

35.
Nilsson G, Svensson V, Nilsson K (1995) Constitutive and inducible cytokine mRNA expression in the human mast cell line HMC-1. Scand J Immunol, 42, 76-81 crossref(new window)

36.
Papanicolaou DA, Wilder RL, Manolagas SC, Chrousos GP (1998) The pathophysiologic roles of interleukin-6 in human disease. Ann Intern Med, 128, 127-137 crossref(new window)

37.
Zhang Y, Ramos BF, Jakschik BA (1992) Neutrophil recruitment by tumor necrosis factor from mast cells inimmune complex peritonitis. Science, 258, 1957-1959 crossref(new window)

38.
Roitt I, Brostoff J, Male D (2002) Immunology, 6th ed, London : Mosby

39.
Hur GM, Ryu YS, Yun HY, Jeon BH, Kim YM, Seok JH, Lee JH (1999) Hepatic ischemia/reperfusion in rats induces iNOS gene transcription by activation of NF-kappaB. Biochem Biophys Res Commun, 261, 917-922 crossref(new window)

40.
Sato T, Nakajima H, Fujio K, Mori Y (1997) Enhancement of prostaglandin E2 production by epidermal growth factor requires the coordinate activation of cytosolic phospholipase A2 and cyclooxygenase 2 in human squamous carcinoma A431 cells. Prostaglandins, 53, 355-369

41.
Huang M, Stolina M, Sharma S, Mao JT, Zhu L, Miller PW, Dubinett SM (1998) Non-small cell lung cancer cyclooxygenase-2-dependent regulation of cytokine balance in lymphocytes and macrophages : up-regulation of interleukin 10 and down-regulation of interleukin 12 production. Cancer Res, 58, 1208-1216