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Effect of garlic (Allium sativum L.) stems on inflammatory cytokines, iNOS and COX-2 expressions in Raw 264.7 cells induced by lipopolysaccharide
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  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 4,  2015, pp.613-621
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.4.613
 Title & Authors
Effect of garlic (Allium sativum L.) stems on inflammatory cytokines, iNOS and COX-2 expressions in Raw 264.7 cells induced by lipopolysaccharide
Cho, Yong Hun; Kim, Hyeon Jeong; Kim, Dong In; Jang, Jae Yoon; Kwak, Jae Hoon; Shin, Yu Hyeon; Cho, Yeon Gje; An, Bong Jeon;
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In this study, the anti-oxidant and anti-inflammatory activities of water extract (ASSW) and 70% ethanol extract (ASSE) of Allium sativum L. stems were investigated using Raw 264.7 cells induced by lipopolysaccharide (LPS). ABTS radical scavenging activities of ASSW and ASSE at concentration were 96.9% and 97.8%, respectively. In order to investigate the potential anti-inflammatory effects of ASSW and ASSE, nitric oxide (NO), pro-inflammatory cytokines, interleukin-6 (IL-6), and tumor necrosis factor including (TNF-), interleukin- (IL-), and prostaglandin-E2 (PGE2) were measured. ASSW and ASSE at concentration showed inhibitory effects against NO production by 18% and 23%, respectively. Production of IL- and IL-6 after treatment with ASSW and ASSE at decreased by approximately 28% and 15% for ASSW and 17% and 12% for ASSE, respectively. In addition, production of TNF- after treatment of of ASSW and ASSE decreased by 24% and 23%, respectively. In addition, the treatment of of ASSW and ASSE showed inhibitory expressions against PGE2 by 45.47% and 33.87%, respectively. These results suggested that ASSE showed greater inhibitory activity than that of the ASSW by the suppression of inflammatory mediators, including NO, IL-6, TNF- and PGE2 production, and the expressions of iNOS and COX-2 in macrophages. In conclusion, ASSW and ASSE may have some ancillary effects on inflammatory factors as potential anti-inflammatory agents.
anti-oxidant;iNOS;COX-2;cytokine;Allium sativum;
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