Advanced SearchSearch Tips
Physicochemical properties of Salvia miltiorrhiza Bunge following treatment with enzymes
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 5,  2015, pp.699-707
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.5.699
 Title & Authors
Physicochemical properties of Salvia miltiorrhiza Bunge following treatment with enzymes
Kim, Sun-Hwa; Hwang, In-Wook; Chung, Shin-Kyo; Seo, Young-Jin; Kim, Jong-Soo; Jeong, Yong-Jin; Kim, Mi-Yeon;
  PDF(new window)
To improve the utilization of the domestic plant Salvia miltiorrhiza Bunge (Danshen), this study investigated changes in the physicochemical qualities of Danshen extracts obtained from low-temperature extraction using the enzymes amylase, cellulase, pectinase, and protease. Changes in the yield, pH, sugar content, and chromaticity were investigated. The changes were found to be highest in the amylase-treated extract with the following values: yield, 58.3%; pH, 6.04; sugar content, . With regard to antioxidant properties, Danshen extracts treated with amylase showed the highest DPPH and ABTS scavenging activities of 84.25% and 74.11% at 55 ppm. The total phenolic compound content was highest in the group subjected to enzyme treatment at . The salvianolic acid B level of the Danshen extract was the highest in the amylase-treated group, with a value of 3,002.6 mg/100 g. Cryptotanshinone level was the highest in the amylase- and protease-treated group with a value of 3.8 mg/100 g. Tanshinone I was the highest in the protease-treated group, with a value of 14.2 mg/100 g. The results showed that the indicator components of Danshen were detected as stable in the extracts after using amylase for low-temperature extraction; therefore, it would be possible to use Danshen industrially as a functional ingredient through mass production. Furthermore, the enzyme-treatment extraction could be utilized for a variety of natural products.
Salvia miltiorrhiza;enzyme treated extract;radical scavenging activity;total phenol content;salvionolic acid B;
 Cited by
Kim KS, Kim SG, Chae SK, Kim BS (2013) Analysis on patent trends in nonthermal processing technologies for medicinal herbs. Korean J Oriental Physiol Pathol, 27, 367-373

King MB, Bott TR (1993) Extraction of natural products using near-critical solvent. Chapman & Hall, London, p 21

Park SH, Hwang HS, Han JH (2004) Development of drink from composition with medicinal plants and evaluation of its physiological function. Korean Nutr Soc, 37, 364-372

Sung NS, Jung HY, Choi JH, Lee SC, Choi BH, Park SS (2014) Preparation of functional healthy drinks by Acanthopanax senticosus extracts. J Life Sci, 24, 959-966 crossref(new window)

Lee SJ, Kim EH, Lee HG (2008) Development of rice wines using Cornus Offinalis and Scutellaria Baicalensis by antioxidant activity tests. Korean J Food Sci Technol, 40, 21-30

Lee JM, Lee HN, Chang YH (2013) Quality characteristics of Makgeolli using Angelica gigas Nakai water extracts. J East Asian Soc Dietary Life, 23, 332-340

Lee JS (2009) Physicochemical properties of Angelica gigas N. and qualitative characteristics of Korea rice cake added with Angelica gigas N. Ph D Thesis, Sejong University, Seoul, Korea

Park GS, An SH (2012) Quality characteristics of pound cake added with Angelica gigas Nakai powder. Korean J Food Cookery Sci, 28, 463-471 crossref(new window)

Kang CS, Lee MJ, Park CB, Bang IS (2012) Study on the antioxidative and physiological activities of Saururus chinensis extract. J Life Sci, 22, 807-814 crossref(new window)

Yang MO (2013) Antioxidant and sensory properties of hot water extract of Liriope Tubers treated at various preprocess. J East Asian Soc Dietary Life, 23, 645-653

Ryu HY, Kim YK, Kwun IS, Kwon CS, Jin IN, Sohn HY (2007) Thrombin inhibition activity of fructus extract of Crataegus pinnatifida Bunge. J Life Sci, 17, 535-539 crossref(new window)

Kim DH, Park JH, Kim JH, Kim CH, You JH, Kwon MC, Lee HY (2005) Enhancement of immune activities of Ephedrae Herba and Rubi Fructus at low temperature extraction. Korean J Medicinal Crop Sci, 13, 81-86

Lee HJ, Jeong HS, Park CG, Lee JH, Park CB, Kim CT, Choi AJ (2014) A study on isolation of polysaccharides from Angelica gigas Nakai by enzyme treatments. Food Eng Prog, 18, 406-412 crossref(new window)

Kwon SC, Choi GH, Hwang JH, Lee KH (2010) Physicochemical property and antioxidative activity of hot-water extracts from enzyme hydrolysate of Astragalus membranaceus. J Korean Soc Food Sci Nutr, 39, 406-413 crossref(new window)

Yeo IH (2012) Effects of Salviae Miltiorrhizae Radix hat aqueous extract on No, PGE2, production and DPPH radical scavenging in macrophage. MS Thesis. Semyung University, Chungbuk, Korea

Han WS, Jeong SI (2000) Studies on the constituents of Salvia militorrhiza Bunge. J Mokwon Institute Natural Science, 9, 11-14

Kim JK (1989) Ilustrated natural drugs encyckopedia, Namsandong Publishers, Seoul, Korea, p 160

Food Code (2011), Ministry of Food and Drug Safety, p 1122

Fugh-Berman A (2000) Herbs and dietary supplements in the prevention and treatment of cardiovascular disease. Prev Cardiol, 3, 24-32 crossref(new window)

Mok JS, Park UY, Kim YM, Chang DS (1994) Effects of solvents and extracting condition on the antimicrobial activity of Salviae miltiorrhizae radix (Salvia miltiorrhiza) extract. J Korean Soc Food Nutr, 23, 1001-1007

Shon YH, Cho HJ, Chang HW, Son KH, Nam KS (2006) Chemopreventive potential of Salviae Miltiorrhizae fraction extracts. J Life Sci, 16, 369-374 crossref(new window)

Jeon SJ (2007) Studies on the chemical analysis and anti-inflammatory acitivities of the components isolated from the Salvia miltiorrhiza Bunge. Ph D Thesis, Andong National University, Gyeongbuk, Korea

Yun HJ, Heo SK, Park WH (2007) Anti-inflammatory effect of Salviae Miltiorrhizae Radix. Korean J Herbol, 22, 65-73

Kim CW (2009) Crytotanshinone, a lipophilic compound of Salvia miltiorriza root, inhibits TNF-${\alpha}$-induced expression of adhesion molecules in HUVEC and attenuates rat myocardial ischemia/reperfusion injury in vivo. MS Thesis. Gyeongsang National University, Gyeongnam, Korea

Yang, WH (2012) Cytotoxic effect of the Salvia miltiorrhiza Bunge extracts on the cancer cell lines. MS Thesis. Dongshin University, Jeonnam, Korea

Lee HJ, Cho JY, Lee SH, Jeon TI, Park KH, Moon JH (2012) Chemical conversion pattern of Salvianolic acid B in aqueous solution under different decoction conditions. Korean J Food Sci Technol, 44, 692-698 crossref(new window)

Lee SE, Cho SI (2015) Anti-inflammatory effects of Salviae Miltiorrhizae Radix extract on RAW 264.7 cell via anti-oxidative activites. Korean J Herbol, 30, 89-94 crossref(new window)

Kwon JH, Belanger JMR, Pare JRJ (2003) Optimization of microwave assisted extraction (MAP) for ginseng components by response surface methodology. J Agric Food Chem, 51, 1807-1810 crossref(new window)

Blosis MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200 crossref(new window)

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTs radical cation decolorization assay. Free Radic Biol Ned, 26, 1231-1237 crossref(new window)

Boo HO, Lee HH, Lee JW, Hwang SJ, Park SU (2009) Different of total phenolics and flavonoids, radical scavenging activities and nitrite scavenging effects of Momordica charantia L. according to cultivars. Korean J Med Crop Sci, 17, 15-20

Lee SH, Kim KN, Cha SH, Ahn GN, Jeon YJ (2006) Comparison of antioxidant activities of enzymatic and methanolic extracts from Ecklonia cava stem and leave. J Korean Soc Food Sci Nutr, 35, 1139-1145 crossref(new window)

Lee JW, Park CK, Do JH (2005) Antioxidative activity of the water soluble browning reaction products isolated from Korean Red Ginseng. J Ginseng Res, 29, 44-48 crossref(new window)

Chaudicre J, Ferrari-Iliou R (1999) Intracellular antioxidants : from chemical to biochemical mechanisms. Food Chem Toxicol 37, 949-962 crossref(new window)

Kim SK, Ban SY, Kim JS, Chung SK (2005) Change of antioxidant activity and antioxidant compounds in Saururus chinensis by extraction conditions. J Korean Soc Appl Biol Chem, 48, 89-92

Kim HK (2009) Anti-inflammatory effect of Liriopis tuber water extract. Ph D Thesis. Kyungwon University, Gyeongbuk, Korea

Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee JS, Jeong HS (2006) Changes of Korean ginseng components with high temperature and pressure treatment. Korean J Food Sci Technol, 38, 521-525

Kim JH, Kang Ym, Eum GS, Ko YM, Kim TY (2003) Antioxidative activity and antimicrobial activity of extracts from medicinal plants (Akebia quinate Decaisn, Scirusfluviatilis A. Gray, Gradenia jasminoides for. grandiflora Makino). J Agric Life Sci, 37, 69-75

Lee HJ, Cho JY, Moon JH (2012) Chemical conversions of Salvianolic acid B by decoction in aqueous solution. Fitoterapia, 83, 1196-1204 crossref(new window)