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Protective effects of mulberry (Morus alba) sugar extracts on hydrogen peroxide-induced oxidative stress in HepG2 cell
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  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 5,  2015, pp.751-757
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.5.751
 Title & Authors
Protective effects of mulberry (Morus alba) sugar extracts on hydrogen peroxide-induced oxidative stress in HepG2 cell
Youn, Young; Kim, Ha-Yan; Park, Hoe-Man; Lee, Sun-Ho; Park, Jong-Ryul; Hong, Seong-Gi; Kim, Young-Geun;
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 Abstract
The objective of this study was to investigate the protective effects of mulberry (Morus alba) sugar extracts (MSE) against -induced oxidative stress in HepG2 cells. The MSEs was mixed with matured mulberry and sugar at the same ratio (1:1, w/w) and stored at for 40 days. In 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging test, MSE stored for 40 days showed high activity with a ratio above 66%. Therefore, we selected 40 days as the optimum storage period. After cell viability analysis using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we determined that the optimum concentration of MSE was 0.5%. Our results showed that MSE increased the cell viability and antioxidant enzyme activities of superoxide dismutase (SOD) and catalase in -treated HepG2 cells. Moreover, the treatment with MSE inhibited malondialdehyde (MDA) levels in -treated HepG2 cells. We also observed a reduction in apoptotic bodies in the Hoechst staining. These data show that MSE treatment significantly suppressed caspase-3 activity in HepG2 cells expored to -induced oxidative stress, thereby indicationg the protective effects of MSE in -induced oxidative stress.
 Keywords
mulberry (Morus alba);sugar extracts;;oxidative stress;
 Language
Korean
 Cited by
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