JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effect of submerged culture of Ceriporia lacerata mycelium on GLUT4 protein in db/db mouse
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 6,  2015, pp.893-900
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.6.893
 Title & Authors
Effect of submerged culture of Ceriporia lacerata mycelium on GLUT4 protein in db/db mouse
Shin, Eun Ji; Kim, Ji-Eun; Kim, Ji-Hye; Park, Yong Man; Yoon, Sung Kyoon; Jang, Byeong-Churl; Lee, Sam-Pin; Kim, Byoung-Cheon;
  PDF(new window)
 Abstract
In this study, we evaluated the antidiabetic effect of a submerged culture of Ceriporia lacerata mycelium (CL01) on hematological indices, as well as protein and mRNA expression of the insulin-signaling pathway, in db/db mice. After CL01 was administrated for 4 weeks, blood glucose levels decreased consistently, and plasma insulin and c-peptide levels each decreased by roughly 55.8%, 40% of those in the negative control (p<0.05). With regard to HOMA-IR, an insulin resistance index, insulin resistance of the CL01-fed group improved over that of the negative control group by about 62% (p<0.05). In addition, we demonstrated that the protein expression levels of pIR, pAkt, pAMPK, and GLUT4 and the mRNA expression levels of Akt2, IRS1, and GLUT4 in the muscle cells of db/db mice increased in the CL01-fed group compared to the corresponding levels in the control group. These results demonstrate that CL01 affects glucose metabolism, upregulates protein and gene expression in the insulin-signaling pathway, and decreases blood glucose levels effectively by improving insulin sensitivity. More than 90% of those who suffer from type 2 diabetes are more likely to suffer from hyperinsulinemia, hypertension, obesity, and other comorbidities because of insulin resistance. Therefore, it is possible that CL01 intake could be used as a fundamental treatment for type 2 diabetes by lowering insulin resistance, and these results may prove be useful as basic evidence for further research into the mechanisms of a cure for type 2 diabetes.
 Keywords
Ceriporia lacerata;diabetes;GLUT4;insulin resistance;
 Language
Korean
 Cited by
1.
Effect of Submerged Culture of Ceriporia lacerata Mycelium on Insulin Signaling Pathway in 3T3-L1 Cell, Journal of Life Science, 2016, 26, 3, 325  crossref(new windwow)
 References
1.
Daisy P, Saipriya K (2012) Biochemical analysis of Cassia fistula aqueous extract and phytochemically synthesized gold nanoparticles as hypolycemic treatment for diabetes mellitus. Int J Nanomedicine, 7, 1189-1202

2.
Korean Statistical Association (2013) Annual report on the cause of death statistics. Korean Statistical Association, Seoul, Korea

3.
Beckman JA, Creager MA, Lbby P (2002) Diabetes and atherosclerosis : epidermiology, pathophysiology, and management. JAMA, 287, 2570-2581 crossref(new window)

4.
Fonseca V (2006) The role of basal insulin therapy in patients with type 2 diabetes mellitus. Insulin, 1, 51-60 crossref(new window)

5.
Goldstein BJ (2002) Insulin resistance as the core defect in type 2 diabetes mellitus. J Cardiol, 90, 3G-10G

6.
Hwang KY, Kim YH, Cho YS, Park YS, Lee JY, Kang KD, Kim K, Joo DK, Seong SI (2008) Hypoglycemic effect of fermented soybean culture mixed with mulberry leaves on neonatal streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr, 37, 452-458 crossref(new window)

7.
Chung MJ, Lee YS, Kim BC, Lee SB, Moon TH, Lee SJ, Park KH (2006) The hypoglycemic effects of acarviosine-glucose modulate hepatic and intestinal glucose transporters in vivo. Food Sci Biotechnol, 15, 851-855

8.
Bell GL, Burant CF, Takeda J, Gould GK (1993) Structure and function of mammalian facilitative sugar transporters. J Biol Chem, 268, 161-164

9.
Belman JP, Habtemichael EN, Bogan JS (2014) A proteolytic pathway that controls glucose uptake in fat and muscle. Endocr Metab Disord, 15, 55-66 crossref(new window)

10.
Ishiki M, Klip A (2005) Minireview : recent developments in the regulation of glucose transporter-4 traffic : new signals, locations, and partners. Endocrinology, 146, 5071-5078 crossref(new window)

11.
Ishikura S, Bilan PJ, Klip A (2007) Rabs 8A and 14 and targets of the insulin-regulated Rab-GAP AS160 regulating GLUT4 traffic in muscle cells. Biochem Biophys Res Commun, 353, 1074-7079 crossref(new window)

12.
Hayashi T, Wojtaszewski JF, Goodyear LJ (1997) Exercise regulation of glucose transport in skeletal muscle. Am J Physiol, 273, 1039-1051

13.
Kang HJ (2007) Exercise physiologic mechanisms related to effective exercise prescription in type 2 diabetes mellitus. Korean J Health Promot Dis Prev, 7, 9-16

14.
Thirone AC, Huang C, Klip A (2006) Tissue-specific roles of IRS proteins in insulin signaling and glucose transport. Trends Endocrinol Metab, 17, 72-78 crossref(new window)

15.
Thong FS, Dugani CB, Klip A (2005) Turning signals on and off : GLUT4 traffic in the insulin-signaling highway. Physiology (Bethesda), 20, 271-284 crossref(new window)

16.
White MF (2003) Insulin signaling in health and disease. Science, 302, 1710-1711 crossref(new window)

17.
Van-pelt RE, Jones PP, Davy KP, Desouza CA, Tanaka H, Davy BM, Seals DR (1997) Regular exercise and the age-related decline in resting metavolic rate in women. J Clin Endocrinol Metab, 82, 3208-3212

18.
Minokoshi Y, Kahn CR, Kahn BB (2003) Tissue-specific ablation of the GLUT4 glucose transporter or the insulin receptor challenges assumptions about insulin action and glucose homeostasis. J Biol Chem, 278, 33609-33612 crossref(new window)

19.
Lee JW, Gwak KS, Park JY, Park MJ, Choi DH, Kwon M, Choi IG (2007) Biological pretreatment of softwood Pinus densiflora by three white rot fungi. J Microbiol, 45, 485-491

20.
Lin Y, He X, Han G, Tian Q, Hu W (2011) Removal of crystal violet from aqueous solution using powdered mycelia biomass of Ceriporia lacerata P2. J Environ Sci, 23, 2055-2062 crossref(new window)

21.
Park JY (2007) Biodegradation of dimethyl phthalate by white rot fungus, Ceriporia lacerata. MS thesis. Seoul National University, Seoul, Korea

22.
Kim JE, Kim HJ, Lee SP (2012) Hyperglycemic effect of submerged culture extract of Ceriporia lacerata in streptozotocin-induced diabetic rats. Food Sci Biotechnol, 21, 1685-1693 crossref(new window)

23.
Shin EJ, Kim JE, Kim JH, Park YM, Yoon SK, Jang BC, Lee SP, Kim BC (2015) Hypoglycemic effect of submerged culture of Ceriporia lacerata mycelium. Korean J Food Preserv, 22, 145-153 crossref(new window)

24.
Wannernes F, Caprio M, Gatta L, Fabbri A, Bonini S, Moretti C (2008) Androgen receptor expression during C2C12 skeletal muscle cell line differentiation. Mol Cell Endocrinol, 292, 11-19 crossref(new window)

25.
Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Tuner RC (1985) Homeostasis model assessment : insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia, 28, 412-419 crossref(new window)

26.
Jung UJ, Lee MK, Jeong KS, Choi MS (2004) The hypoglycemic effects of hesperidin and naringin are partly mediated by hepatic glucose-regulating enzymes in C57BL/KsJ-db/db mice. J Nutr 134, 2499-2503

27.
Remsberg KE, Talbott EO, Zborowski JV, Evans RW, McHugh-Pemu K (2002) Evidence for competing effects of body mass, hyperinsulinemia, insulin resistance, and androgens on leptin levels among lean, overweight, and obese women with polycystic ovary syndrome. Fertil Steril, 78, 479-486 crossref(new window)

28.
Haffner SM, Stern MP, Hazuda HP, Mitchell BD, Patterson JK (1990) Cardiovascular risk factors in confirmed pre-diabetic individuals : does the clock for coronary heart disease start ticking before the onset of clinical diabetes? JAMA, 263, 2893-2898 crossref(new window)

29.
Kim JS, Park KS Lee YY (1998) Characteristic of insulin resistance in Korea. Diabetes, 22, 84-91

30.
Ha JH, Lee SH (2010) Role of AMPK in the regulation of cellular energy metabolism. Endocrinol Metab, 25, 9-17

31.
Yuan Z, He P, Cui J, Takeuchi H (1998) Hypoglycemic effect of water-soluble polysaccharide from Auricularia-judae Quel. on genetically diabetic KK-A mice. Biosci Biotechnol Biochem, 62, 1898-1903 crossref(new window)

32.
Kiho T, Sobue S, Ukai S (1994) Structural features and hypoglycemic activities of two polysaccharides from a hot-water extract of Agrocybe cylindracea. Carbohydr Res, 251, 81-87 crossref(new window)

33.
Kim JH, Park YK, Kim JE, Lee SP, Kim BC, Jang BC (2013) Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity. Int J Mol Med, 32, 179-186