Advanced SearchSearch Tips
Allergic risk assessment of genetically modified cucumber mosaic virus resistant pepper
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 6,  2015, pp.901-907
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.6.901
 Title & Authors
Allergic risk assessment of genetically modified cucumber mosaic virus resistant pepper
Son, Dae-Yeul;
  PDF(new window)
Genetically modified (GM) pepper H15 containing the gene for cucumber mosaic virus (CMV) coat protein (CP) and its control line non-GM pepper P2377 were investigated for their allergic risk. Amino acid sequence of the inserted gene product CMV-CP was compared with those of known allergens. No known allergen had greater than 35% amino acid sequence homology over an 80 amino acid window or more than 8 consecutive identical amino acids. Protein patterns of GM and non-GM pepper extracts were evaluated by SDS-PAGE, which showed similar distribution of protein bands for both GM and non-GM pepper. Antigen-antibody reactions were compared between GM and its non-transgenic parental control. ELISA and immunoblot analysis of sera from allergic patients showed some IgE reactivity; however, no differences were observed between GM pepper H15 and P2377. We therefore conclude that CMV-CP is less likely to be an allergen; the protein composition and allergenicity of the GM pepper H15 is not different from that of P2377 and safe as a commercial host.
CMV resistant;safety assessment;allergenicity;
 Cited by
IFT's Expert Panel on Food Safety and Nutrition (1985) Food Allergen and Sensitivities. Food Technol, 39, 65-71

Metcalfe D, Astwood J, Townsend R (1996) Assessment of the allergenic potential of food derived from genentically engineered crop plants. Crit Rev Food Sci Nutr, 36, 165-186 crossref(new window)

Gendel S (1998) Sequence databases for assessing the potential allergenicity of proteins used in transgenic foods. Adv Food Nutr Res, 42, 63-92 crossref(new window)

Son DY, Lee BR, Shon DW, Lee KS, Ahn KM, Nam SY, Lee SI (2000) Allergenicity change of soybean proteins by thermal treatment. Korean J Food Sci Tenchnol, 32, 959-963

Astood JD, Leach JN, Fuchs RL (1996) Stability of food allergens to digestion in vitro. Nat Biotechnol, 14, 1269-1273 crossref(new window)

Fu TJ, Abbott UR, Hatzos C (2002) Digestibility of food allergens and non-allergenic proteins in simulated gastric fluid and simulated intestinal fluid- a comparative study. J Agric Food Chem, 50, 7154-7160 crossref(new window)

Lee SH, Lee JB, Kim SM, Choi HS, Park JW, Lee JS, Lee KW, Moon JS (2004) The incidence and distribution of viral diseases in pepper by cultivation types. Res Plant Dis, 10, 231-240 crossref(new window)

Guil-Guerrero JL, Martinez-Guirado C, Rebolloso-Fuentes M del Mar, Carrique-Perez A (2006) Nutrient composition and antioxidant activity of 10 pepper (Capsicum annuum) varieties. Eur Food Res Technol, 224, 1-9 crossref(new window)

Lee JH, Hong JS, Ju HJ, Park DH (2015) Occurrence of viral diseases in field-cultivated pepper in Korea from 2006 to 2010. Korean J Organic Agri, 23, 123-131 crossref(new window)

Kim DH, Cho JD, Kim JH, Kim JS, Cho EK (2005) Ultrastructural characteristics of necrosis and stunt disease in red pepper by the mixed infections of Tobacco mosaic virus-U1 and Pepper mild mottle virus and Pepper mottle virus. Plant Pathol J, 21, 252-257 crossref(new window)

Alonso E, Gracia LI, Avila RMJ, Wicke B, Serra MYT, Diaz RJR (1989) A tobamovirus causing heavy losses in protected pepper crops in Spain. J Phytopathol, 125, 67-76 crossref(new window)

Pares RD (1985) A tobamovirus infecting capsicum in Australia. Ann Appl Biol, 106, 459-474

Schuerger AC, Hammer W (1995) Effects of temperature on disease development of tomato mosaic virus in Capsicum annuum in hydroponic systems. Plant Dis, 79, 880-885 crossref(new window)

Lee YH, Jung M, Shin SH, Lee JH, Choi SH, Her NH, Lee JH, Ryu KH, Paek KY, Harn CH (2009) Transgenic peppers that are highly tolerant to a new CMV pathotype. Pant Cell Rep, 28, 223-232 crossref(new window)

Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72, 248-254 crossref(new window)

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 crossref(new window)

Codex Alimentarius (2009) Foods derived from modern biotechnology. Codex Alimentarius Commission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organization of the United Nations, Rome, Italy

Hileman RE, Silvanovich A, Goodman RE,Rice EA, Holleschak G, Astwood JD, Hefle SL (2002) Bioinformatic methods for allergenicity assessment using a comprehensive allergen database. Int Arch Allergy Immunol 128, 280-291 crossref(new window)

Lee YH, Jung M, Shin SH, Lee JH, Choi SH, Her NH, Lee JH, Ryu KH, Paek KY, Harn CH (2009) Transgenic peppers those are highly tolerant to a new CMV pathotype. Plant Cell Rep, 28, 223-232 crossref(new window)

Cho DW, Oh JP, Park KW, Chung KH (2009) Analysis of agricultural characteristics for the virus resistant GM pepper plants grown in three different regions in Korea. Korean J Hort Sci Technol, 27, 521-529

Bang SN, Jung YS, Eom SJ, Kim GB, Chung KH, Lee GP, Son DY, Park KW, Hong JS, Ryu KH, Lee C (2012) Assessment of the cucumber mosaic virus coat protein by expression evaluation in a genetically modified pepper and Escherichia coli BL21. J Food Biochem, 36, 432-440 crossref(new window)