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Allergic risk assessment of genetically modified cucumber mosaic virus resistant pepper
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  • Journal title : Korean Journal of Food Preservation
  • Volume 22, Issue 6,  2015, pp.901-907
  • Publisher : The Korean Society of Food Preservation
  • DOI : 10.11002/kjfp.2015.22.6.901
 Title & Authors
Allergic risk assessment of genetically modified cucumber mosaic virus resistant pepper
Son, Dae-Yeul;
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 Abstract
Genetically modified (GM) pepper H15 containing the gene for cucumber mosaic virus (CMV) coat protein (CP) and its control line non-GM pepper P2377 were investigated for their allergic risk. Amino acid sequence of the inserted gene product CMV-CP was compared with those of known allergens. No known allergen had greater than 35% amino acid sequence homology over an 80 amino acid window or more than 8 consecutive identical amino acids. Protein patterns of GM and non-GM pepper extracts were evaluated by SDS-PAGE, which showed similar distribution of protein bands for both GM and non-GM pepper. Antigen-antibody reactions were compared between GM and its non-transgenic parental control. ELISA and immunoblot analysis of sera from allergic patients showed some IgE reactivity; however, no differences were observed between GM pepper H15 and P2377. We therefore conclude that CMV-CP is less likely to be an allergen; the protein composition and allergenicity of the GM pepper H15 is not different from that of P2377 and safe as a commercial host.
 Keywords
CMV resistant;safety assessment;allergenicity;
 Language
Korean
 Cited by
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