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Effects of Plant Growth Regulators on in vitro Propagation of Echinosophora koreensis Nakai
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 Title & Authors
Effects of Plant Growth Regulators on in vitro Propagation of Echinosophora koreensis Nakai
Yi, Jae-Seon; Lee, Hyunseok; An, Chanhoon;
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To establish in vitro nodal culture conditions of Echinosophora koreensis Nakai, one of rare and endangered species famous for beautiful flowers in the Korean Peninsula, the influence of plant growth regulators (PGRs) on shooting and rooting from in vitro shoots was investigated. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the half-strength Driver and Kuniyuki`s media in the range of 2.22 to 8.88 induced 2.5 to 2.7 shoots per axillary bud; and addition of 2.27 thidiazuron (TDZ) produced 3.2 shoots, during 4 weeks of culture, while zeatin and isopentenyl adenine (2ip) were not effective on shoot multiplication as observed from several combination treatments of BA with other PGRs. Shoots established were smaller than 2 cm in length, in most of the treatments. while in BA 8.88 treatment more than 30% of shoots were longer than 2 cm and shorter than 4 cm. In rooting, naphthalene acetic acid (NAA) from 5.37 to 21.48 showed the rooting rate from 40.0 to 62.5%. Indole butyric acid (IBA) addition had little effect on rooting (<10%), although some roots in IBA-containing media were longer than those in NAA. Micropropagation from axillary buds of nodular explants was applicable and promising to multiplication and conservation of Echinosophora koreensis Nakai.
Echinosophora koreensis Nakai;axillary bud;shoot development;rooting;
 Cited by
Ahmed MR, Anis M. 2012. Role of TDZ in the quick regeneration of multiple shoots from nodal explant of Vitex trifolia L.--an important medicinal plant. Appl Biochem Biotechnol 168: 957-966. crossref(new window)

Amutha S, Muruganantham M, Ganapathi A. 2006. Thidiazuroninduced high-frequency axillary and adventitious shoot regeneration in Vigna radiata (L.) Wilczek. In Vitro Cell Dev Biol - Plant 42: 26-30. crossref(new window)

Brassard N, Brissette L, Lord D, Laliberte S. 1996. Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch. PCTOC 44: 37-44. crossref(new window)

Cheon KS, Jang SK, Lee WT, Yoo KO. 2009. The natural habitat and distribution of Echinosophora koreensis (Nakai) Nakai in Korea. Korean J Pl Taxon 39: 254-263.

Choi SW, Lim S, Park WG, Choi YE. 2011. Plant regeneration from the segments of petioles of Cacalia firma. Korean J Plant Res 24: 483-488. crossref(new window)

Chung JD, Harm SH, Jee SO, Chung MY, Khin TM, Kim CK. 2004. The multiple propagation of mericlones from shoot tip culture of Neofinetia falcate. J Kor Flower Res Soc 12: 101-106.

Driver JA, Kuniyuki AH. 1984. In vitro propagation of Paradox walnut Juglans hindsii${\time}$Juglans regia rootstock. Hort Science 19: 507-509.

Han MS, Moon HK, Kang YJ, Kim WW, Kang BS, Byun KO. 2004. Micropropagation of an Endangered Species, Stellera rosea Nakai by Tissue Culture. Korean J Plant Biotechnol 31: 31-35. crossref(new window)

Kim CM, Lee KB. 1990. A study on chemical constituents and biological activity of Echinosophora koreensis Nakai. Kor J Pharmacogn 21: 137-141.

Kim HJ, Cho HJ, Kim EY, Kim MY, Park HB. 2007. Mass propagation by in vitro culture of Bupleurum latissimum Nakai. Korean J Plant Res 20: 367-374.

Kavitha M, Kalaimagal I, Mercy S, Sangeetha N, Ganesh D. 2009. In vitro plant regeneration from apical bud and nodal segments of Anthocepahalus cadamba - An important sacred and medicinal tree. J For Sci 25: 111-118.

Khalafalla MM, Hattori K. 1999. A combination of thidiazuron and benzyladenine promotes nultiple shoot production from cotyledonary node explants of faba bean (Vicia faba L.). Plant Growth Regulation 27: 145-148. crossref(new window)

Khan MI, Anis M. 2012. Modulation of in vitro morphogenesis in nodal segments of Salix tetrasperma Roxb. through the use of TDZ, different media types and culture regimes. Agrofor Syst 86: 95-103. crossref(new window)

Koh GC, Lim DO. 2006. In vitro propagation of Korean native winter hazel (Corylopsis coreana). Korean J Hort Sci Technol 24: 272-278.

Kumar N, Reddy MP. 2011. In vitro plant propagation: A review. J For Sci 27: 61-72.

Lee JS, Han SW, Kim HJ, Lee NY. 2007. Effect of cutting period and IBA, NAA, rooton on the rooting of Hydrangea serrata. J Korean Soc People Plants Environ 10: 131-134.

Lee JG, Lee BY. 2009. Effect of rooting promoter and root zone temperature controls on growth and rooting of highbush blueberry cuttings. Korean J Hort Sci Technol 27: 7-11.

Ministory of Environment. 1997. The study of growth characteristic of nation's major plants and best management practicestrees and shrubs (in Korean). pp. 274-275.

Moon HK, Kim YW. 2008. In vitro propagation of a rare and endangered species, Echinosophora koreensis Nakai, by axillary bud culture. J Plant Biotechnol 35: 229-234. crossref(new window)

Moon HK, Kim SJ, Park SY, Kim YW, Yi JS. 2009. Effect of plant growth regulators on micropropagation of a rare and endangered species, Tsuru-rindo (Tripterospermum japonicum). J Plant Biotechnol 36: 13-17. crossref(new window)

Mukhtar S, Ahmad N, Khan MI, Anis M, Aref IM. 2012. Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types. Physiol Mol Biol Plants 18: 381-386. crossref(new window)

Oh BU, Han JW, Yang SK, Jang ES, Jang CG, Kim YY, Ji SJ, Kang SH. 2009. Flora and Vegetation in a Habitat of Echinosophora koreensis (Nakai) Nakai (Leguminosae), a Korean Endemic Plant in Yanggu-gun (Kangwon), Korea -Focused on Jukgok-ri and Hwanggang-ri-. J Korean Env Res Tech 12: 19-28.

Park YY, Cho MS, Lee YD, Chung JB, Park S, Jeong BR, Gyu S. 2007. Effect of BA concentrations and culture methods on in vitro plant multiplication from shoot-tip culture of wasabia japonica. J Plant Biotechnol 34: 1-6. crossref(new window)

Shim KK, Ha YM, Son CJ, Han DS, Lee SA. 2006. A study on development of materials for native landsape tree-native area survey and characteristic of Korean endemic plant, Echinosophora koreensis Nakai-. J Kor Inst Traditional Landscape Architecture 24: 32-42.

Valero-Aracama C, Kane ME, Wilson SB, Philman NL. 2010. Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult-and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes. Plant Growth Regulation 60: 43-49. crossref(new window)

26. 2010-07-06 10:00. Discovered Echinosophora koreensis Nakai. which is one of the Korea endemic species in Jiri mountain by Sung, Hwan-Gil. html?mode=view&cat=13&nid=132404. Accessed 1 Jun 2010.