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In vitro Propagation and Protocorm-like Body Formation of Endangered Species, Dendrobium moniliforme
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 Title & Authors
In vitro Propagation and Protocorm-like Body Formation of Endangered Species, Dendrobium moniliforme
Bae, Kee Hwa; Kim, Nam Young; Song, Jae Mo; Song, Gwanpil;
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 Abstract
This experiment was conducted to establish the in vitro propagation of Dendrobium moniliforme through protocorm like body (PLB) induction from the culture of leaf, stem and root explants. Frequency of PLB formation from root explants was higher than those of leaf and stem explants. PLB`s proliferation in MS medium including 1.0 mg/L NAA was better than 1.0 mg/L IBA. Frequency of PLB`s proliferation on medium with various concentrations of BA, Kinetin, Tidiazuron (0. 1.0, 3.0 mg/L) and NAA (0, 1.0 mg/L) was tested. The maximun proliferation of PLB`s was obtained on MS medium supplemented with 3.0 mg/L BA and 1.0 mg/L NAA after 6 weeks of culture. Addition of 200 mg/L activated charcoal (AC) and 30 g/L sucrose was effective on PLB proliferation for D. moniliforme.
 Keywords
activated charcoal;Dendrobium moniliforme;endangered;protocorm like body;sucrose;
 Language
Korean
 Cited by
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