JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Characterization of In vitro Propagated Plants Via Somatic Embryo Formation from Old Wild Panax ginseng
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Characterization of In vitro Propagated Plants Via Somatic Embryo Formation from Old Wild Panax ginseng
Bae, Kee Hwa; Choi, Yong Eui;
  PDF(new window)
 Abstract
Wild Korean ginseng has been recognized as highly precious medicine since ancient times. Nowadays, the population of wild ginseng in the forest of Korean peninsula is very rare due to indiscreet harvest. In this work, we investigated the plant regeneration via somatic embryogenesis from embryogenic callus of old wild ginseng (more than 50 years-old) and compared the features of plants regenerated from 5-years old and 50 years-old ginseng. Induction of embryogenic callus from adventitious roots of 50 year-old wild ginseng required 83 weeks of culture, but only 10 weeks were sufficient for 5 year-old ginseng. Height and width of plants derived from the old wild ginseng was smaller and slender compared to the plantlets derived from 5 year-old ginseng. Total chlorophyll contents was 2-6 time lower in plantlets regenerated from 50 year-old wild ginseng than those from 5 year-old ginseng, but anthocyanin content was higher in 50 year-old ginseng. Our results revealed that plants regenerated from old wild ginseng have different morphological and physiological characters probably due to age-dependent phenomenon.
 Keywords
plant regeneration;somatic embryogenesis;wild ginseng;forest cultivated ginseng;embryogenic callus;
 Language
English
 Cited by
 References
1.
Ahn IO, Le BV, Gendy C, Tran Thanh Van K. 1996. Direct somatic embryogenesis through thin cell layer culture of Panax ginseng. Plant Cell Tissue Organ Cult 45: 237-243. crossref(new window)

2.
Ando T, Tanaka O, Shibata S. 1971. Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Syoyakugaku Zasshi 25: 28-32.

3.
Arya S, Arya ID, Eriksson T. 1993. Rapid multiplication of adventitious somatic embryos of Panax ginseng. Plant Cell Tissue Org Cult 34: 157-162. crossref(new window)

4.
Bahler BD, Steffen KL, Orzolek MD. 1991. Morphological and biochemical comparison of a purple-leafed and a green-leafed pepper cultivar. HortScience 26: 736.

5.
Chalker-Scott L. 1999. Environmental significance of anthocyanins in plant stress responses. Photochemistry and Photobiology 70: 1-9. crossref(new window)

6.
Chalker-Scott L. 2002. Do anthocyanins function as osmoregulators in leaf tissues? Advances in Botanical Research 37: 103-106. crossref(new window)

7.
Chang WC, Hsing YI. 1980. Plant regeneration through somatic embryogenesis in root-derived callus of ginseng (Panax ginseng C. A. Meyer). Theor Appl Genet 57: 133-135.

8.
Choi KT, Kim MW, Shin HS. 1981. Root and shoot formation in explant and callus derived from root and cotyledon of ginseng (Panax Ginseng C. A. Meyer). Korean J Ginseng Sci 5: 35-40.

9.
Choi YE, Yang DC, Park JC, Soh WY, Choi KT. 1998. Regenerative ability of somatic single and multiple embryos from cotyledons of Korean ginseng on hormone-free medium. Plant Cell Rep 17: 544-551. crossref(new window)

10.
Choinski JS, Johnson JM. 1993. Changes in photosynthesis and water status of developing leaves of Brachystegia spiciformis Benth. Tree Physiol 13: 17-27. crossref(new window)

11.
Close DC, Beadle CL. 2003. The ecophysiology of foliar anthocyanin. Botanical Review 69: 149-161. crossref(new window)

12.
Gould KS, Markham KR, Smith RH, Goris JJ. 2000. Functional role of anthocyanins in the leaves of Quintinia serrata A. Cunn. J Exp Bot 51: 1107-1115. crossref(new window)

13.
Han JY, Jung SJ, Kim SW, Kwon YS, Yi MJ, Yi JS, Choi YE. 2006. Induction of adventitious roots, analysis of ginsenoside and genes involved in triterpene biosynthesis in Panax ginseng. J Plant Biol 49: 26-33. crossref(new window)

14.
Hoch WA, Zeldin EL, McCown BH. 2001. Physiological significance of anthocyanins during autumnal leaf senescence. Tree Physiol 21: 1-8. crossref(new window)

15.
Lee HS, Liu JR, Yang SG, Lee YH, Lee KW. 1990. In vitro flowering of plantlets regenerated from zygotic embryo-derived somatic embryos of ginseng. HortScience 25: 1652-1654.

16.
Lichtenthaler HK. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods Enzymol 148: 350-382. crossref(new window)

17.
Mehrtens F, Kranz H, Bednarek P, Weisshaar B. 2005. The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis. Plant Physiol 138: 1083-1096. crossref(new window)

18.
Merzlyak MN, Chivkunova OB, Solovchenko AE, Naqvi KR. 2008. Light absorption by anthocyanins in juvenile, stressed, and senescing leaves. J Exp Bot 59: 3903-3911. crossref(new window)

19.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497. crossref(new window)