Advanced SearchSearch Tips
Characterization of Tetracycline-Resistant Genes of Staphylococci isolates from Human and Marine Environment
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Characterization of Tetracycline-Resistant Genes of Staphylococci isolates from Human and Marine Environment
CHO, Ki-Taek; KIM, Young-Chul; KWON, Woo-Ju; JEONG, Hyun-Do;
  PDF(new window)
For comparison of tetracycline-resistant () genes, we obtained 21 and 14 Staphylococcus spp. from marine environment and human patient, respectively. Although all isolates from human were identified as Staphylococcus aureus, higher proportion of isolates (12 out of 14) from human were utilizing tet(M) gene compared to that of isolates (6 out of 21) from marine environment. Additionally, collaborated utilization of tet(M) and erm(A) in S. aureus in human patient, but not in Staphylococcus spp. isolates from marine environment was also characterized. Based on the nucleotide sequence of transposon related to gene, we confirmed the origin of tet(M) gene in Staphylococci isolated from marine environments and human are derived from Tn916/1545-like and Tn5801 transposon, respectively. It is the first report showing the presence of Tn5801 in all S. aureus carrying tet(M) in human patient. Alignment of the fully sequenced tet(M) from marine environmental isolates was also agreed with the determined transposons by showing the genomic mosaic structure composed with three genomic parts from Tn916/1545 and unknown transposons. Genetic characteristics of these tet(M) in environmental isolates were similar to each other but different from those in isolates from human showing only tet(M) from Tn916/5801 type. It may imply the presence of less dramatic communication of antibiotic resistant genes between Staphylococci isolated from marine environment and human.
Resistance genes;tet(M);Staphylococci;Transposon;
 Cited by
CLSI. (2012). Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement. Document M100-S22. CLSI, Wayne PA Vol. 32

Courvalin, P, Carlier, C.(1987). Tn1545: a conjugative shuttle transposon. Mol. Gen. Genet 206, 259-264. crossref(new window)

De Vries, L. E..Christensen, H..Skov, R. L..Aarestrup, F. M..Agerso, Y.(2009). Diversity of the tetracycline resistance gene tet(M) and identification of Tn916- and Tn5801-like (Tn6014) transposons in Staphylococcus aureus from humans and animals. J Antimicrob Chemother 64(3), 490-500. crossref(new window)

Eady, E. A..Ross, J. I..Tipper, J. L..Walters, C. E..Cove, J. H..Noble, W. C.(1993). Distribution of genes encoding erythromycin ribosomal methylases and an erythromycin efflux pump in epidemiologically distinct groups of staphylococci. J Antimicrob Chemother 31(2), 211-217. crossref(new window)

Fernandez-Astorga, A..Muela, A..Cisterna, R..Iriberri, J..Barcina, I.(1992). Biotic and abiotic factors affecting plasmid transfer in Escherichia coli strains. Appl Environ Microbiol 58, 392-398.

Fujioka, R. S..Unutoa, T. M.(2006). Comparative stability and growth requirements of S. aureus and faecal indicator bacteria in seawater. Water Sci Technol 54(3),169-175.

Huys, G..D'Haene, K..Collard, J. M..Swings, J.(2004). Prevalence and molecular characterization of tetracycline resistance in Enterococcus isolates from food. Appl Environ Microbiol 70(3), 1555-1562. crossref(new window)

Kuroda, M..Ohta, T..Uchiyama, I..Baba, T..Yuzawa, H..Kobayashi, I..Cui, L..Oguchi, A..Aoki, K..Nagai, Y..Lian, J..Ito, T..Kanamori, M..Matsumaru, H..Maruyama, A..Murakami, H..Hosoyama, A..Mizutani-Ui. Y..Takahashi, N. K..Sawano, T..Inoue, R..Kaito, C..Sekimizu, K..Hirakawa, H..Kuhara, S..Goto, S..Yabuzaki, J..Kanehisa, M..Yamashita, A..Oshima, K..Furuya, K..Yoshino, C..Shiba, T..Hattori, M..Ogasawara, N..Hayashi, H..Hiramatsu, K.(2001). Whole genome sequencing of methicillin-resistant Staphylococcus aureus. Lancet 357, 1225-1240. crossref(new window)

Lee, J. Y..Park, J. H..Moon, K. H.(2007). Antibiotic Resistance in Staphylococcus aureus isolated in Busan. Yakhak hoeji 51(3), 164-168.

Neela, F. A..Nonaka, L..Rahman, M. H..Suzuki, S.(2009). Transfer of the chromosomally encoded tetracycline resistance gene tet(M) from marine bacteria to Escherichia coli and Enterococcus faecalis. World J Microbiol Biotechnol 25, 1095-1101. crossref(new window)

Oggioni, M. R..Dowson, C. G..Smith, J. M..Provvedi, R..Pozzi, G.(1996). The tetracycline resistance gene tet(M) exhibits mosaic structure. Plasmid 35(18), 156-163. crossref(new window)

Oppegaard, H..Steinum, T. M..Wasteson, Y.(2001). Horizontal transfer of a multi-drug resistance plasmid between coliform bacteria of human and bovine origin in a farm environment. Appl Environ Microbiol 67(8), 3732-3734. crossref(new window)

Park, J. H..Lee, J. Y. and Moon, K. H.(2008). Characterization of tetracycline resistance plasmids of Staphylococcus aureus. Yakhak Hoeji 52(4), 279-282.

Rice, L. B.(1998). Tn916 family conjugative transposons and dissemination of antimicrobial resistance determinants. Antimicrob Agents Chemother 42(8), 1871-1877.

Rizzotti, L., La Gioia, F., Dellaglio, F., Torriani, S.(2009). Molecular diversity and transferability of the tetracycline resistance gene tet(M), carried on Tn916-1545 family transposons, in enterococci from a total food chain. Antonie Van Leeuwenhoek 96(1),43-52. crossref(new window)

Schmitz, F. J..Krey, A..Sadurski, R..Verhoef, J..Milatovic, D..Fluit, A. C. ; European SENTRY Participants.(2001). Resistance to tetracycline and distribution of tetracycline resistance genes in European Staphylococcus aureus isolates. J Antimicrob Chemother 47(2), 239-240. crossref(new window)

Schwarz, S..Roberts, M. C..Werckenthin, C..Pang, Y..Lange, C.(1998). Tetracycline resistance in Staphylococcus spp. from domestic animals. Vet Microbiol 63(2-4), 217-227. crossref(new window)

Soge, O. O..Meschke, J. S..No, D. B..Roberts, M. C. (2009). Characterization of methicillinresistant Staphylococcus aureus and methicillinresistant coagulase-negative Staphylococcus spp. isolated from US West Coast public marine beaches. J Antimicrob Chemother 64(6), 1148-1155. crossref(new window)

Trzcinski, K..Cooper, B. S..Hryniewicz, W..Dowson, C. G.(2000). Expression of resistance to tetracyclines in strains of methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother 45(6), 763-770. crossref(new window)

Warsa, U. C..Nonoyama, M..Ida, T..Okamoto, R..Okubo, T..Shimauchi, C..Kuga, A..Inoue, M.(1996). Detection of tet(K) and tet(M) in Staphylococcus aureus of Asian countries by the polymerase chain reaction. J Antibiot (Tokyo) 49(11), 1127-1132. crossref(new window)