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Expression of Catalase (CAT) and Ascorbate Peroxidase (APX) in MuSI Transgenic Tobacco under Cadmium Stress
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 Title & Authors
Expression of Catalase (CAT) and Ascorbate Peroxidase (APX) in MuSI Transgenic Tobacco under Cadmium Stress
Kim, Kye-Hoon; Kim, Young-Nam; Lim, Ga-Hee; Lee, Mi-Na; Jung, Yoon-Hwa;
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 Abstract
The MuSI is known as a multiple stress resistant gene with several lines. A previous study using RT-PCR showed that the expression of MuSI gene in tobacco plant induced its tolerance to Cd stress. This study was conducted to examine the enhanced Cd tolerance of the MuSI transgenic tobacco plant through germination test and to understand the role of the involved antioxidant enzymes for the exhibited tolerance. Germination rate of MuSI transgenic tobacco was more than 10% higher than that of wild-type tobacco, and seedlings of MuSI transgenic tobacco grew up to 1.6 times larger and greener than seedlings of wild-type tobacco at 200 and 300 Cd. From the third to the fifth day, CAT activities at 100 and 200 Cd and APX activities at 100, 200 and 300 Cd of MuSI transgenic tobacco were up to two times higher than those of wild-type tobacco. MuSI gene is shown to enhance the activities of antioxidant enzymes resulting in higher tolerance to oxidative stress compared with the control plant.
 Keywords
MuSI transgenic tobacco;Cadmium resistance;Catalase (CAT);Ascorbate Peroxidase (APX);
 Language
English
 Cited by
1.
우리나라 농경지 중금속 동태 및 작물흡수 연구동향,이지호;김지영;고우리;정은정;;정구복;김두호;김원일;

한국환경농학회지, 2012. vol.31. 1, pp.75-95 crossref(new window)
2.
토양 중 고농도 카드뮴에 노출된 MuS1 형질전환 담배 (Nicotiana tabacum cv. Xanthi)의 생리적 반응 및 카드뮴 축적: 식물학적 오염토양정화를 위한 형질전환 식물 탐색,정윤화;김영남;김권래;김계훈;

한국토양비료학회지, 2013. vol.46. 1, pp.58-64 crossref(new window)
1.
Physiological Response and Cadmium Accumulation of MuS1 Transgenic Tobacco Exposed to High Concentration of Cd in Soil: Implication to Phytoremediation of Metal Contaminated Soil, Korean Journal of Soil Science and Fertilizer, 2013, 46, 1, 58  crossref(new windwow)
2.
Catalase and ascorbate peroxidase—representative H2O2-detoxifying heme enzymes in plants, Environmental Science and Pollution Research, 2016, 23, 19, 19002  crossref(new windwow)
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