Advanced SearchSearch Tips
Effects of Supplementation of Mixed Methanogens and Rumen Cellulolytic Bacteria on Biochemical Methane Potential
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Supplementation of Mixed Methanogens and Rumen Cellulolytic Bacteria on Biochemical Methane Potential
Kim, Ji-Ae; Yoon, Young-Man; Kim, Chang-Hyun;
  PDF(new window)
The study investigated the biochemical methane potential (BMP) assay of cellulose supplementing with mixed methanogens and cellulolytic bacteria to improve anaerobic digestion for methane production. For the BMP assay, 7 different microbial supplementation groups were consisted of the cultures of mixed methanogens (M), Fibrobacter succinogenes (FS), Ruminococcus flavefaciensn (RF), R. albus (RA), RA+FS and M+RA+FS including control. The cultures were added in the batch reactors with the increasing dose levels of 1% (0.5 mL), 3% (1.5 mL) and 5% (2.5 mL). Incubation for the BMP assay was carried out for 40 days at and anaerobic digestate obtained from an anaerobic digester with pig slurry as inoculum was used. In results, 5% FS increased total biogas and methane production up to 10.4~22.7% and 17.4~27.5%, respectively, compared to other groups (p<0.05). Total solid (TS) digestion efficiency showed a similar trend to the total biogas and methane productions. Generally the TS digestion efficiency of the FS group was higher than that of other groups showing at the highest value of 64.2% in the 5% FS group. Volatile solid (VS) digestion efficiencies of 68.4 and 71.0% in the 5% FS and the 5% RF were higher than other groups. After incubation, pH values in all treatment groups were over 6.4 indicating that methanogensis was not inhibited during the incubation. In conclusion, the results indicated that the hydrolysis stage for methane production in anaerobic batch reactors was the late-limiting stage compared with the methanogenesis stage, and especially, as the supplementation levels of F. succinogenes supplementation increased, the methane production was increased in the BMP assay compared with other microbial culture addition.
BMP (biochemical methane potential);Methanogens;Rumen cellulolytic bacteria;Fiberobacter succinogenes;
 Cited by
양돈슬러리를 이용한 혐기소화에서 미생물 첨가가 메탄발생에 미치는 영향,김지애;윤영만;정광화;김창현;

한국토양비료학회지, 2012. vol.45. 6, pp.1049-1057 crossref(new window)
Effects of Supplementation of Mixed Methanogens and Rumen Cellulolytic Bacteria on Biochemical Methane Potential with Pig Slurry, Korean Journal of Soil Science and Fertilizer, 2012, 45, 6, 1049  crossref(new windwow)
Angelidaki, I. and B.K. Ahring. 2000. Methods for increasing the biogas potential from the recalcitrant organic matter contained in manure. Water Sci. Technol. 41:189-194.

Angelidaki, I., S.P. Petersen, and B.K. Ahring. 1990. Effects of lipids on thermophilic and anaerobic digestion and reduction of lipid inhibition upon addition of bentonite. Appl. Microbiol. Biotechnolol. 33:469-472.

APHA. 1998. Standard methods for the examination of water and wastewater. (20th ed.) American Public Health Association, Washington, DC, USA.

Beuvink, J.M., S.F. Spoelstra, and R.J. Hogendrop. 1992. An automated method of measuring the time course of gas production of feedstuffs incubated with buffered rumen fluid. Neth. J. Agri. Sci., 40:401-407.

Bonmati A., X. Flotats, L. Mateu, and E. Campos. 2001. Study of thermal hydrolysis as a pretreatment to mesophilic anaerobic digestion of pig slurry. Water Sci. Technol. 44:109-116.

Bryant, M.P., N. Small, C. Bouma, and I.M. Robinson. 1958. Studies on the composition of the ruminal flora and fauna of young calves. J. Dairy Sci. 41:1747-1767. crossref(new window)

Chynoweth, D.P., C.E. Turick, J.M. Owens, D.E. Jerger, and M.W. Peck. 1993. Biochemical methane potential of biomass and waste feedstocks. Biomass Bioenerg. 5:95-111. crossref(new window)

Clemens, J., M. Trimborn, P. Weiland, and B. Amon. 2006. Mitigation of greenhouse gas emissions by anaerobic digestion of cattle slurry. Agri. Ecosyst. Environ. 112:171-177. crossref(new window)

Danish Energy Agency. 1992. Update on centralized biogas plants.

Dehority, B.A. 1963. Isolation and characterization of several cellulolytic bacteria from in vitro rumen fermentations. J. Dairy Sci. 46:217-222. crossref(new window)

Dehority, B.A. 2003. Rumen microbiology. Nottingham University Press, Nottingham, UK.

Duncan, D.B. 1955. Multiple range and multiple F tests. Biometrics. 11:1. crossref(new window)

Gerardi, M.H. 2003. The microbiology of anaerobic digesters. John Wiley & Sons, Inc., New York, USA.

Gijen, H.J., K.B. Zwart, P.T., van Gelder, and G.D. Vogels. 1986. Continuous cultivation of rumen microorganisms, a system with possible application to the anaerobic degradation of lignocellulosic waste materials, Appl. Micro. Biotech. 25:155-162. crossref(new window)

Hashimoto, A.G. 1989. Effect of inoculum/substrate ratio on methane yield and production rate from straw. Biol. Wastes. 28:247-255. crossref(new window)

Hungate, R.E. 1947. Studies on cellulose fermentation. III. The culture and isolation of cellulose-decomposing bacteria from the rumen of cattle. J. Bacteriol. 53:631-645.

Hungate, R.E. 1950. The anaerobic mesophilic cellulolytic bacteria. Bacteriol. Rev. 14:1-49.

Hungate, R.E. 1963. Polysaccharide storage and growth efficiency in Ruminococcus albus. J. Bacteriol. 86:848-854.

Lawrence, A.W. and P.L. McCarty. 1967. Kinetics of methane fermentation in anaerobic waste treatment. Technical report No. 75. Stanford, Califonia, USA.

Leslie Grady, C.P., G.T. Daigger, and H.C. Lim. 1999. Biological Wastewater Treatment (2nd ed). p. 599-604. Marcel Dekker, Inc., NY, USA.

Lettinga, G. 2001. Digestion and degradation, air for life. Water Sci. Technol. 44: 157-176.

Ministry of Environment. 2009. Environmental white paper. ISBN 11-1480000-000586-10 (In Korean).

Muller, H.W. and W. Trosch, 1986. Screening of white-rot fungi for biological pretreatment of wheat straw for biogas production. Appl. Micro. Biotech. 24:180-185. crossref(new window)

Odenyo, A.A., R.I. Mackie, and B.A. White. 1994. The use of 16S rRNA-targeted oiligonucleotide probes to study competition between ruminal fibrolytic bacteria: pure-culture studies with cellulose and alkaline peroxide-treated wheat straw. Appl. Environ. Microbiol. 60:3697-3703.

Owen, W.P., D.C. Stuckey, J.B. Healy, L.Y. Young, and P.L. McCarty. 1979. Bioassay for monitoring biochemical methane potential and anaerobic toxicity. Water Res. 13:485-492. crossref(new window)

Rymer, C., and D.I. Givens. 2002. Relationships between patterns of rumen fermentation measured in sheep and in situ degradability and the in vitro gas production profile of the diet. Anim. Feed. Scie. Technol. 101:31-44. crossref(new window)

SAS. 1999. Statistical Analysis Systems User's Guide. (8th ed.) SAS Institute Inc. Raleigh, NC, USA.

Shelton, D.R. and J. Tiedije. 1984. General method for determining anaerobic biodegradation potential. Appl. Environ. Microbiol., 47:850-857.

Schulman, M.D. and D. Valentino. 1976. Factors influencing rumen fermentation: effect of hydrogen on formation of propionate. J. Dairy. Sci. 59:1444-1451. crossref(new window)

Shin, H.S. Y.C. Song, and K.S. Jun. 1992. Pretreatment processes for enhanced anaerobic digestion of food waste. p. 451-454. In F. Cecchi et al. (ed.) Proceedings of international symposium on anaerobic digestion of solid waste. Venice, Italy.

Speece, R. 1996. Anaerobic biotechnology for industrial wastewaters. p. 29-58. Archae Press, Nashville, TN, USA.

Theodorou, M.K., D.R. Daivies, B.B. Nilsen, M.I.G. Lawrence, and A.P.J. Trinci. 1998. Principles of techniques that rely on gas measurement in ruminant nutrition. p. 55-63. E.R. Deaville et al. (ed.) In vitro techniques for measuring nutrient supply to ruminants. (Occasional publication, No. 22). British Society of Animal Science, UK.

Theodorou, M.K., B.A. Williams, M.S. Dhanoa, A.B. McAllan, and J. France. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim. Feed. Sci. Technol. 48:185-197. crossref(new window)

van Lier J.B., A. Tilche, B.K. Ahring, H. Macarie, R. Moletta, M. Dohanyo, L.W. Hulshoff Pol, P. Lens, and W. Werstraete. 2001. New perspectives in anaerobic digestion. Water Sci. Techno. 43:1-18.

Williams, A., M. Amat-Marco, and M.D. Collins. 1996. Pylogenetic analysis of Butyrivibrio strains reveals three distinct groups of species within the Clostridium subphylm of gram-positive bacteria. Int. J. Syst. Bacterol. 46:195-199. crossref(new window)