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Assessment of Microbial Community in Paddy Soils Cultivated with Bt and Nakdong Rice
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 Title & Authors
Assessment of Microbial Community in Paddy Soils Cultivated with Bt and Nakdong Rice
Sohn, Soo-In; Ahn, Byung-Ohg; Chi, Hee-Youn; Cho, Byung-Kwan; Cho, Min-Seok; Shin, Kong Sik;
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The cultivation of genetically modified (GM) crops has increased due to their economic and agronomic advantages. Before commercialization of GM crops, however, we must assess the potential risks of GM crops on human health and environment. The aim of this study was to investigate the possible impact of Bt rice on the soil microbial community. Microbial communities were isolated from the rhizosphere soil cultivated with Bt rice and Nakdong, parental cultivar and were subjected to be analyzed using both culture-dependent and molecular methods. The total counts of bacteria, fungi, and actinomycetes in the rhizosphere of transgenic and conventional rice were not significantly different. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that the bacterial community structures during cultural periods were very similar each other. Analysis of dominant isolates in the rhizosphere cultivated with Bt and Nakdong rice showed that the dominant isolates from the soil of Bt rice and Nakdong belonged to the Proteobacteria, Cloroflexi, Actinobacteria, Firmicutes, and Acidobacteria. These results indicate that the Bt rice has no significant impact on the soil microbial communities during cultivation period. Further study remains to be investigated whether the residue of Bt rice effect on the soil environment.
Bt rice;Soil microbial community;16S rDNA;pyrosequencing;
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Bashir, K., T. Husnain, T. Fatima, Z. Latif, S.A. Mehdi, and S. Riazuddin. 2004. Field evaluation and risk assessment of transgenic indica basmati rice. Mol. Breed. 13:301-312. crossref(new window)

Betz, F. S., B.G. Hammond, and R. L. Fuchs. 2000. Safety and advantages of Bacillus thringiensis-protected plants to control insect pests. Regul. Toxicol. Pharmacol. 32: 156-173. crossref(new window)

Carpenter, J. E. 2010. Peer-reviewed surveys indicate positive impact of commercialized GM crops. Nat. Biotechnol. 28: 319-321. crossref(new window)

Clark, M. S., M. S. Smith, and J. W. Doran. 1998. Changes in soil chemical properties resulting from organic and low-input farming practices. Agron. J. 90: 662-671. crossref(new window)

de Vries, J. and W. Wackernagel. 2004. Microbial horizontal gene transfer and the DNA release from transgenic crop plants. Plant Soil. 266: 91-104.

Duformantel, N., G. Tissot, F. Goutorbe, F. Garcon, C. Muhr, S. Jansens, B. Pelissier, G. Peltier, and M. Dubald. 2005. Generation and analysis of soybean plastid transformants expressing Bacillus thuringiensis Cry1Ab protoxin. Plant Mol. Biol. 58: 659-668. crossref(new window)

Estruch, J. J., G. W. Warren, M. A. Mullins, G. J. Nye, J. A. Craig, and M. G. Koziel. 1996. Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a spectrum of activities against lepidopteran insect. Proc. Natl. Acad. Sci. USA. 93: 5389-5394. crossref(new window)

He, K., Z. Wang, S. Bai, L. Zheng, Y. Wang, and H. Cui. 2006. Efficacy of transgenic Bt cotton resistance to the Asian corn borer (Lepidoptera: Crambidae). Crop Prot. 25: 167-173. crossref(new window)

Icoz, I., D. Saxena, D.A. Andow, C. Zwahlen, and G. Stotzky. 2008. Microbial populations and enzyme activities in soil in situ under transgenic corn expressing cry proteins from Bacillus thuringiensis. J. Environ. Qual. 37(2), 647-662. crossref(new window)

Icoz, I. and G. Stotzky. 2008. Fate and effects of insect-resistant Bt crops in soil ecosystems. Soil Biol. Biochem. 40:559-586. crossref(new window)

James, C. 2011. Global status of commercialized biotech/GM crops: 2011. ISAAA Briefs No. 43, Ithaka, NY.

Jung, B. G., G. H. Jo, E. S. Yun, J. H. Yoon, and Y. H. Kim. 1998. Monitoring on chemical properties of bench marked paddy soils in Korea. Korean J. Soil Sci. Fert. 31(3) 246-252.

Kim, E. S., S. W. Hong, and K. S. Chung. 2011. Comparative analysis of bacterial diversity in the intestinal tract of earthworm (Eisenia fetida) using DGGE and pyrosequencing. Korean J. Microbiol. Biotechnol. 39(4): 374-381

Kim E. H., S. C. Suh, B. S. Park, K. S. Shin, S. J. Kweon, E. J. Han, S. H. Park, Y. S. Kim, and J. K. Kim. 2009. Chloroplast-targeted expression of synthetic cry1Ac in transgenic rice as an alternative strategy for increased pest protection. Planta. 230: 397-405. crossref(new window)

Kim, Y.J. and K.S. Whang. 2007. Phylogenetic characteristics of viable but nonculturable bacterial populations in a pine mushroom (Tricholloma matcutake) forest soil. The Korean J. Microbiol. 43: 201-209.

Koziel, M. G., G. L. Beland, C. Bowman, N. B. Carozzi, R. Crenshaw, L. Crossland, J. Dawson, N. Desai, M. Hill, S. Kadwell, K. Lauris, K. Lewis, D. Maddox, K. McPherson, M. R. Meghji, E. Merlin, R. Rhodes, G. W. Warren, M. Wright, and S. V. Evola. 1993. Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Bio. Technol. 11: 194-200. crossref(new window)

Kumar, H. and V. Kumar. 2004. Tomato expressing Cry1A(b) insecticidal protein from Bacillus thuringiensis protected against tomato fruit borer, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) damage in the laboratory, greenhouse and field. Crop Protect. 23:135-139. crossref(new window)

Lee, S. H., C. G. Kim, and H. J. Kang. 2011. Temporal dynamics of bacterial and fungal communities in a genetically modified(GM) rice ecosystem. Microb. Ecol. 61: 646-659. crossref(new window)

Liu, W., H. H. Lu, W. Wu, Q. K. Wei, Y. X. Chen, and J. E. Thies. 2008. Transgenic Bt rice does not affect enzyme activities and microbial composition in the rhizosphere during crop development. Soil Biol. Biochem. 40: 475-486. crossref(new window)

Lu, H., W. Wu, Y Chen, H. Wang, M. Devare, and J. E. Thies. 2010. Soil microbial community responses to Bt transgenic rice residue decomposition in a paddy field. J. Soils Sediments. 10:1598-1605. crossref(new window)

Maqbool, S. B., S. Riazuddin, N. T. Loc, A. M. R. Gatehouse, J. A. Gatehouse, and P. Christou 2001. Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. Mol. Breed. 7: 85-93. crossref(new window)

Meissle, M., E. Vojtech, and G. M. Poppy. 2005. Effects of Bt maize-fed prey on the generalist predator Poecilus cupreus L. (Coleoptera: Carabidae). Transgenic Res. 14:123-132. crossref(new window)

Mendelsohn, M., J. Kough, Z. Vaituzis, and K. Matthews. 2003. Are Bt crops safe? Nature Biotech. 21: 1003-1009. crossref(new window)

Nap, J.P., J. Bijvoet, and W. J. Stiekema. 1992, Biosafety of kanamycin-resistant transgenic plants. Transgenic Res. 1: 239-249. crossref(new window)

NAAS (National Academy of Agricultural Science). 2000. Analysis method of soil and plant: Physics, chemistry and microorganism. RDA, Korea.

Odum. 1998. Fundamental of ecology. Gadjah Mada University Press. Yogyakarta. 800p.

Perlak, F. J., R. W. Deaton, T. A. Amstrong, R. L. Fuchs, S. R. Sims, J. T. Greenplate, and D. A. Fischhoff. 1990. Insect resistant cotton plants. Bio. Technol. 8: 939-943. crossref(new window)

Raney, T. 2006. Economic impact of transgenic crops in developing contries. Curr. Opin. Plant Biol. 17: 1-5.

Raybould, A. and D. Vlachos. 2011. Non-target organism effects rests on Vip3A and their application to the ecological risk assessment for cultivation of MIR162 maize. Transgenic Res. 20: 599-611. crossref(new window)

Shu, Q., G. Ye, H. Cui, X. Cheng, Y. Xiang, D. Wu, M. Gao, Y. Xia, C. Hu, R. Sardana, and I. Altosaar. 2000. Transgenic rice plants with a synthetic cry1Ab gene Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species. Mol. Breed. 6: 433-439. crossref(new window)

Sohn, S. I., Y. J. Oh, S. D. Oh, M. K. Kim, T. H. Ryu, K. J. Lee, S. C. Suh, H. J. Baek, and J. S. Park. 2010. Molecular analysis of microbial community in soils cultivating Bt Chinese cabbage. Korean J. Environ. Agric. 29(3): 293-299. crossref(new window)

Stewart, C. N., M. D. Jr., Halfhill, and S. I. Warwick. 2003. Transgene introgression from genetically modified crops to their wild relatives. Nat. Rev. Gen. 4: 806-817. crossref(new window)

Tu, J., G. Zhang, K. Datta, C. Xu, Y. He, Q. Zhang, G. S. Khush, and S. K. Datta. 2000. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis $\delta$-endotoxin. Nat. Biotechnol. 18:1101-1104. crossref(new window)

Wei, M., F. Tan, H. Zhu, K. Cheng, X. Wu, J. Wang, K. Zhao, and X. Tang. 2012. Impact of Bt-transgenic rice (SHK601) on soil ecosystems in the rhizosphere during crop development. Plant Soil Environ. 58(5): 217-223.

Ye, G. Y., Q. Y. Shu, H. W. Yao, H. R. Cui, X. Y. Cheng, C. Hu, Y. W. Xia, M. W. Gao, and I. Altosaar. 2001. Field evaluation of resistance of transgenic rice containing a synthetic cry1Ab gene from Bacillus thuringiensis Berliner to two stem borers. J. Econ. Entomol. 94: 271-276. crossref(new window)

Ye, G. Y., H. W. Yao, Q. Y. Shu, X. Cheng, C. Hu, Y. W. Xia, M. W. Gao, and I. Altosaar. 2003. High levels of stable resistance in transgenic rice with a cry1Ab gene from Bacillus thuringiensis Berliner to rice leaffilder, Cnaphalocrocis medinalis (Guenee) under field conditions. Crop Protect. 22: 171-178. crossref(new window)