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Assessment of Microbial Community in Paddy Soils Cultivated with Bt and Nakdong Rice
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 Title & Authors
Assessment of Microbial Community in Paddy Soils Cultivated with Bt and Nakdong Rice
Sohn, Soo-In; Ahn, Byung-Ohg; Chi, Hee-Youn; Cho, Byung-Kwan; Cho, Min-Seok; Shin, Kong Sik;
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 Abstract
The cultivation of genetically modified (GM) crops has increased due to their economic and agronomic advantages. Before commercialization of GM crops, however, we must assess the potential risks of GM crops on human health and environment. The aim of this study was to investigate the possible impact of Bt rice on the soil microbial community. Microbial communities were isolated from the rhizosphere soil cultivated with Bt rice and Nakdong, parental cultivar and were subjected to be analyzed using both culture-dependent and molecular methods. The total counts of bacteria, fungi, and actinomycetes in the rhizosphere of transgenic and conventional rice were not significantly different. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that the bacterial community structures during cultural periods were very similar each other. Analysis of dominant isolates in the rhizosphere cultivated with Bt and Nakdong rice showed that the dominant isolates from the soil of Bt rice and Nakdong belonged to the Proteobacteria, Cloroflexi, Actinobacteria, Firmicutes, and Acidobacteria. These results indicate that the Bt rice has no significant impact on the soil microbial communities during cultivation period. Further study remains to be investigated whether the residue of Bt rice effect on the soil environment.
 Keywords
Bt rice;Soil microbial community;16S rDNA;pyrosequencing;
 Language
Korean
 Cited by
1.
Bt 양배추가 복숭아혹진딧물의 발육과 기주선택에 미치는 영향,남기정;김영중;문두범;남경희;백인순;박정호;정순천;한지학;김창기;

한국응용곤충학회지, 2014. vol.53. 2, pp.193-197 crossref(new window)
2.
국화의 GM 이벤트 선발을 위한 실질적 동등성 및 환경위해성 평가,임진희;심명선;오권헌;서지연;이수영;박상근;신학기;조명래;

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Assessment of Substantial Equivalence and Environmental Risk for Event Selection of Genetically Modified Chrysanthemum, Korean Journal of Horticultural Science and Technology, 2014, 32, 4, 517  crossref(new windwow)
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