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Toxicity Assessment of Silver Ions Compared to Silver Nanoparticles in Aqueous Solutions and Soils Using Microtox Bioassay
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 Title & Authors
Toxicity Assessment of Silver Ions Compared to Silver Nanoparticles in Aqueous Solutions and Soils Using Microtox Bioassay
Wie, Min-A; Oh, Se-Jin; Kim, Sung-Chul; Kim, Rog-Young; Lee, Sang-Phil; Kim, Won-Il; Yang, Jae E.;
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This study was conducted to assess the microbial toxicity of ionic silver solution () and silver nanoparticle suspension () based on the Microtox bioassay. In this test, the light inhibition of luminescent bacteria was measured after 15 and 30 min exposure to aqueous solutions and soils spiked with a dilution series of and . The resulting dose-response curves were used to derive effective concentration (EC25, , EC75) and effective dose (, , ) that caused a 25, 50 and 75% inhibition of luminescence. In aqueous solutions, value of after 15 min exposure was determined to be < and remarkably lower than value of with . This revealed that was more toxic to luminescent bacteria than . In soil extracts, however, value of with 196 mg kg-1 was higher than value of with , indicating less toxicity of in soils. The reduced toxicity of in soils can be attributed to a partial adsorption of ionic on soil colloids and humic acid as well as a partial formation of insoluble AgCl with NaCl of Microtox diluent. This resulted in lower concentration of active Ag in soil extracts obtained after 1 hour shaking with than that spiked with . With longer exposure time, EC and ED values of both and decreased, so their toxicity increased. The toxic characteristics of silver nanomaterials were different depending on existing form of Ag (, ), reaction medium (aqueous solution, soil), and exposure time.
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수생태계에서 ZnO, TiO2나노입자 응집체가 물벼룩(Daphnia magna)에 미치는 영향,이하늘;이병우;박찬일;김무찬;

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