JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Toxicity Assessment of Silver Ions Compared to Silver Nanoparticles in Aqueous Solutions and Soils Using Microtox Bioassay
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Toxicity Assessment of Silver Ions Compared to Silver Nanoparticles in Aqueous Solutions and Soils Using Microtox Bioassay
Wie, Min-A; Oh, Se-Jin; Kim, Sung-Chul; Kim, Rog-Young; Lee, Sang-Phil; Kim, Won-Il; Yang, Jae E.;
  PDF(new window)
 Abstract
This study was conducted to assess the microbial toxicity of ionic silver solution () and silver nanoparticle suspension () based on the Microtox bioassay. In this test, the light inhibition of luminescent bacteria was measured after 15 and 30 min exposure to aqueous solutions and soils spiked with a dilution series of and . The resulting dose-response curves were used to derive effective concentration (EC25, , EC75) and effective dose (, , ) that caused a 25, 50 and 75% inhibition of luminescence. In aqueous solutions, value of after 15 min exposure was determined to be < and remarkably lower than value of with . This revealed that was more toxic to luminescent bacteria than . In soil extracts, however, value of with 196 mg kg-1 was higher than value of with , indicating less toxicity of in soils. The reduced toxicity of in soils can be attributed to a partial adsorption of ionic on soil colloids and humic acid as well as a partial formation of insoluble AgCl with NaCl of Microtox diluent. This resulted in lower concentration of active Ag in soil extracts obtained after 1 hour shaking with than that spiked with . With longer exposure time, EC and ED values of both and decreased, so their toxicity increased. The toxic characteristics of silver nanomaterials were different depending on existing form of Ag (, ), reaction medium (aqueous solution, soil), and exposure time.
 Keywords
AgNP;dose-response curve;;;microtox;
 Language
Korean
 Cited by
1.
수생태계에서 ZnO, TiO2나노입자 응집체가 물벼룩(Daphnia magna)에 미치는 영향,이하늘;이병우;박찬일;김무찬;

해양환경안전학회지, 2014. vol.20. 5, pp.468-473 crossref(new window)
1.
Dose–response effects of silver nanoparticles and silver nitrate on microbial and enzyme activities in calcareous soils, Geoderma, 2017, 285, 313  crossref(new windwow)
2.
Effect of Daphnia magna on Nanoparticle(ZnO, TiO2) Aggregates in Aqueous System, Journal of the Korean Society of Marine Environment and safety, 2014, 20, 5, 468  crossref(new windwow)
 References
1.
AZUR Environmental. 1998. The Microtox acute basic, DIN, ISO and wet test procedure. Carlsbad, Calif, USA.

2.
Cho, G.Y. 2011. Chemicals management division. Ministry of Environment. Korea.

3.
Feng, Q.L., J. Wu, G.Q. Chen, F.Z. Cui, T.N. Kim, and J.O. Kim. 2000. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J. Biomed. Mater. Res. 52:662-662. crossref(new window)

4.
Haensch, M. and C. Emmerling. 2010. Effects of silver nanoparticles on the microbiota and enzyme activity in soil. J. Plant Nutiri. Soil Sci. 173:554-558. crossref(new window)

5.
Jacobson, A.R., S. Klitzke, M.B. McBride, P. Baveye, and T.S. Steenhuis. 2005. The desorption of silver and thallium from soils in the presence of a chelating resin with thiol functional groups. Water Air Soil Poll. 160:41-54. crossref(new window)

6.
Jovanovic, B., L. Anastasova, E.W. Rowe, Y. Zhang, A.R. Clapp, and D. Palic. 2011. Effects of nanosized titanium dioxide on innate immune system of fathead minnow (Pimephales promelas Rafinesque, 1820). Ecotoxicol Environ Saf. 74(7):675-683. crossref(new window)

7.
Lee, S.P. 2006. Ecotoxicological assessment of soils contaminated with heavy metals using soil enzymes and luminescent bacteria. Master-Thesis. Kangwon National University.

8.
Lee, J.N. and I.Y. Hwang. 1999. Evaluation of environmental toxicities for priority water pollutants in a small watershed by bioassays. Kor. J. Environ. Toxicol. 14:135-144.

9.
Lee, Y.S., H.S. Jeong, S.W. Kim, M.S. Yeo, H.S. Lim, E.J. Kim, and J.S. Min. 2005. Inhibition effect of nano-silver liquid against various plant microorganisms. J. Agri. Life Environ. Sci. 16:125-133.

10.
Liau, S.Y., D.C. Read, W.J. Pugh, J.R. Furr, and A.D. Russell. 1997. Interaction of silver-nitrate with readily identifiable groups; relationship to the antibacterial action of silver ions. Lett. Appl. Microbiol. 25:297-283.

11.
MOE. 2009a. Soil contamination standards methods. 2009.09. Ministry of Environment, Gwacheon. Korea.

12.
MOE. 2009b. Enforcement Decree of the Soil Environment Conservation Act. 12th. amended. No. 333. 2009.6.25. Ministry of Environment, Gwacheon. Korea.

13.
MOE. 2011. Soil monitoring network and survey of soil pollution status. Annual report 2010. Ministry of Environment, Gwacheon. Korea.

14.
Morones, R., J.L. Elechiguerra, A. Camacho, K. Holt, J.B. Kouri, J.T. Ramiyrez, and M.J. Yacaman. 2005. The bactericidal effect of silver nanoparticles. Nanotechnology 16:2346-2353. crossref(new window)

15.
Nam, J.J, S.J. Kim, and P.K. Park. 2006. Microtox biological toxic assessment of soils treated with sewage sludge. J. Korean Soc. Environ. Anal. 9(3):191-198.

16.
NIAST. 2000. Methods of soil and plant anlysis. National Institut of Agricultural Science and Technology, RDA, Suwon, Korea (In Korean).

17.
NIER. 2010. Study on hazardous properties of manufactured silver nanoparticles. National Institute of Environmental Research.

18.
Park, K. 2005. Toxicity of nanomaterials and strategy of risk assessment. K. Environ. Toxicol. 20(4):259-271.

19.
Park, Y.H. 2008. Impact of the nano-silver suspension on soil quality parameters. Master-Thesis. Kangwon National University.

20.
Salizzato, M., B. Pavoni, A.V. Ghirardini, and P.F. Ghetti. 1998. Sediment toxicity measured using Vibrio fischeri as related to the concentrations of organic (PCBs, PAHs) and inorganic (Metals, sulphur) pollutants. Chemosphere 36:2949-2968. crossref(new window)

21.
Slawson, R.M., H. Lee, and J.T. Trevors. 1990. Bacterial interactions with silver. Biol. Met. 3:151-154. crossref(new window)

22.
Sondi, I. and B.S. Sondi. 2004. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram-negative bacteria. J. Colloid Interface Sci. 275:177- 182. crossref(new window)

23.
Throbaeck, I.N., M. Johansson, M. Rosenquist, M. Pell, M. Hansson, and S. Hallin. 2007. Silver (Ag+) reduces denitrification and induces enrichment of novel nirK genotypes in soil. FEMS Microb. Lett. 270:189-194. crossref(new window)

24.
Woodrow Wilson Database. 2011. Nanotechnology consumer product inventory. http: //www.nanotechproject.org/inventories/ consumer/analysis_draft/.