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Effect of Temperature on Survival of Escherichia coli O157:H7 and Listeria monocytogenes in Livestock Manure Compost
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 Title & Authors
Effect of Temperature on Survival of Escherichia coli O157:H7 and Listeria monocytogenes in Livestock Manure Compost
Jung, Kyu-Seok; Heu, Sung-Gi; Roh, Eun-Jung; Kim, Min-Ha; Gil, Hyun-Ji; Choi, Na-Young; Lee, Dong-Hwan; Lim, Jeong-A; Ryu, Jae-Gee; Kim, Kye-Hoon;
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Animal manure compost is a commonly used fertilizer in organic vegetable and fruit production in Korea. However, livestock manure compost produced from animal feces can contain a lot of the non-pathogenic and pathogenic bacteria. Of particular concern are bacteria causing human food-borne illness such as Escherichia coli O157:H7 and Listeria monocytogenes. The objective of this study was to investigate effect of temperature on survival of E. coli O157:H7 and L. monocytogenes in livestock manure compost. Commercial livestock manure compost (manure 60%, sawdust 40%) was inoculated with E. coli O157:H7 and L. monocytogenes. Compost was incubated at four different temperatures (10, 25, 35, and ) for 20 weeks. Samples were taken every week during incubation depending on the given conditions. E. coli O157:H7 persisted for up to 1 day in livestock manure compost at , over 140 days at , 140 days at , and 120 days at , respectively. L. monocytogenes persisted for up to 1 day in livestock manure compost at and 140 days at , 70 days at , and 40 days at , respectively. The results indicated that E. coli O157:H7 and L. monocytogenes persisted longer under low temperature condition. E. coli O157:H7 survived longer than L. monocytogenes at three different temperatures (10, 25, and ). The results are being used to develop guidelines on the management of manure to reduce the risks of E. coli O157:H7 and L. monocytogenes transmission to foods produced in the presence of animal waste.
Livestock manure compost;Escherichia coli O157:H7;Listeria monocytogenes;Survival;
 Cited by
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