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Microbial Contamination Levels of Strawberries at Domestic Farms of South Korea
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 Title & Authors
Microbial Contamination Levels of Strawberries at Domestic Farms of South Korea
Kim, Won-Il; Jo, A-Ra; Kim, Se-Ri; Ryu, Song Hee; Nam, Ki-Woong; Yoon, Yohan; Yoon, Deok-Hoon; Oh, So-Yong; Nam, Myeong Hyeon; Ryu, Jae-Gee; Kim, Hwang-Yong;
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Foodborne illness due to the consumption of contaminated raw strawberries is a continuing food safety concern. This study investigated and evaluated contamination levels of bacteria on strawberries at farms stage to evaluate potential hazards associated with fresh strawberries. A total of 315 samples, 105 samples from 5 sampling sites (A to E) of 21 farms and 210 samples from 1 sampling site of 6 farms, was collected every month for four months and analyzed to enumerate aerobic bacterial counts, Coliforms/E. coli, Bacillus cereus and Staphylococcus aureus. In addition, the prevalence study of five pathogens (S. aureus, E. coli, E. coli O157:H7, Salmonella spp. and Listeria monocytogenes) was performed on each sample. Aerobic bacterial counts ranged from 0.48 to 6.36 Log CFU/g, with the highest bacterial cell counts recorded for D and E sites. Coliforms were detected in 71 samples (22.5%) with a minimum of 0.48 cfu/g and a maximum of more than 4 Log CFU/g. B. cereus was detected in 98 samples (31.1%) among total samples analyzed. S. aureus was detected in 2 samples with a minimum of 0.48 Log CFU/g and a maximum of 1.38 Log CFU/g. E. coli, E. coli O157:H7, Salmonella spp. and L. monocytogenes were not isolated from any of the samples. The microbial contamination levels of strawberries determined in this study may be used as the fundamental data for microbiological risk assessment.
Strawberry;Microbiological quality;Food-borne pathogen;
 Cited by
Tian, J. Q., Bae, Y. M., Choi, N. Y., Kang, D. H., Heu, S. and Lee, S. Y. 2012. Survival and growth of foodborne pathogens in minimally processed vegetables at 4 and $15^{\circ}C$. J. Food Sci., 77(1):M48-M50. crossref(new window)

RDA(Rural Development Administration). 2013. Strawberry, pp. 20.

Statistics Korea. Korean Statistical Information Service (

Lee, C. Y., Lee, W. G., Song, J. E., Kim, K. Y., Shim, W. B., Yoon, Y. H., Kim, Y. S. and Chung, D. H. 2012. Hazard analysis for the cultivation stage of strawberry farms for securing preliminary data to establish the Good Agricultural Practices. J. Agric. Life Sci., 46(3):97-108.

Centers for Disease Control and Prevention (CDC). 2007. Morbidity and mortality weekly report (MMWR). 56(35):909-911.

The Marler Clark Network: Foodborne illness outbreak database. http://outbreakdatabase. com.

Koseki, S. and Isobe, S. 2005. Prediction of pathogen growth on iceberg lettuce under real temperature history during distribution from farm to table. Int. J. Fd. Microbiol., 104:239-248. crossref(new window)

Ackers, M. L., Mahon, B. E., Leahy, E., Goode, B. Damrow, T., Hayes, P. S., Bibb, W. F., Rice, D. H., Barrett, T. J., Hutwagner, L., Griffin, P. M. and Slutsker, L. 1998. An outbreak of Escherichia coli O157:H7 infections associated with leaf lettuce consumption. J. Infect. Dis., 177:1588-1593. crossref(new window)

Beuchat, L. R. 1996. Listeria monocytogenes: incidence on vegetables. Food Control, 7, 223-228. crossref(new window)

De Roever, C. 1998. Microbiological safety evaluations and recommendations on fresh produce. Food Control, 9:321-347. crossref(new window)

Mead, P. S., Slutsker, L. Dietz, V., McCaig, L. F., Bresee, J. S., Shapiro, C., Griffin, P. M. and Tauxe, R. V. 1999. Food-related illness and death in the United States. Emerg. Infect. Dis., 5:607-625. crossref(new window)

Beuchat, L. R. and Scouten, A. J. 2002. Combined effects of water activity, temperature and chemical treatments on the survival of Salmonella and Escherichia coli O157:H7 on alfalfa seeds. J. Appl. Microbiol, 92:382-395. crossref(new window)

Shim, W. B., Kim, K. Y., Yoon, Y. H., Kim, J. E., Shim, S. I., Kim, Y. S. and Chung, D. H. 2013. Microbiological hazard analysis for strawberry farms at the harvest stage to establish Good Agricultural Practices (GAP) model based on principle of HACCP. Korean J. Food Sci. Technol., 45(1):104-110. crossref(new window)

Lee, C. Y., Lee, W. G., Song, J. E., Kim, K. Y., Shim, W. B., Yoon, Y. H., Kim, Y. S. and Chung, D. H. 2012. Hazard analysis for the cultivation stage of strawberry farms for securing preliminary data to establish the Good Agricultural Practices. J. Agriculture & Life Science, 46(3):97-108.

Yu, Y. M., Youn, Y. N., Hua, Q. J., Cha, G. H. and Lee, Y. H. 2009. Biological hazard analysis of paprikas, strawberries and tomatoes in the markets. J. Fd Hyg. Safety., 24:174-181.

Yu, Y. M., Kim, J. W., Choi, I. W., Youn, Y. N. and Lee, Y. H. 2013. Bacterial contamination levels in strawberry parts according to their cultivation methods. Korean J Food Preserv, 20(3): 323-329. crossref(new window)

Kim, W. I., Jung, H. M., Kim, S. R., Park, K. H., Kim, B. S., Yun, J. C. and Ryu, K. Y. 2012. Investigation of microbial contamination levels of leafy greens and its distributing conditions at different time - Focused on perilla leaf and lettuce -. J. Fd Hyg. Safety, 27(3):277-284. crossref(new window)

Kang, S.T. and Yun, J.Y. 2004. Food Microbiology. pp. 414-430.

Tyrrel, S.F., Knox, J.W. and Weatherhead, E.K. 2006. Microbiological water quality requirements for salad irrigation in the United Kingdom. J. Food Prot., 69(8):2029-2035.

Mitra, R., Cuesta-Alonso, E., Wayadande, A., Talley, J., Gilliland, S. and Fletcher, J. 2009. Effect of route of introduction and host cultivar on the colonization, internalization, and movement of the human pathogen Escherichia coli O157:H7 in spinach. J. Food Prot., 72:1521-1530.

Steele, M. and Odumeru, J. 2004. Irrigation water as source of foodborne paghogens on fruit and vegetables. J Food Prot., 67(12):2839-2849.

Wachtel, M. R. and Charkowski, A. O. 2002. Cross-contamination of lettuce with Escherichia coli O157:H7. J Food Prot., 65:465-470.

Kroupitski, Y., Pinto, R., Brandl, M. T., Belausov, E. and Sela, S. 2009. Interactions of Salmonella enterica with lettuce leaves. J. Appl Microbiol, 106:1876-1885. crossref(new window)

Ukuku, D. O. and Sapers, G. M. 2007. Effect of time before storage and storage temperature on survival of Salmonella inoculated on fresh-cut melons. Food Microbiol, 24:288-295. crossref(new window)

Gagliardi, J. V., Millner, P. D., Lester, G. and Ingram, D. 2003. On-farm and postharvest processing sources of bacterial contamination to melon rinds. J. Food Prot., 66:82-87.

Calder, L., Simmons, G., Thornley, C., Taylor, P., Pritchard, K., Greening, G. and Bishop, J. 2003. An Outbreak of Hepatitis A Associated with Consumption of Raw Blueberries. Epidemiol. Infect. 131(1):745-751. crossref(new window)

CDC (Centers for Disease Control and Prevention). Foodborne outbreak online database (FOOD). Data retrieved from

Uyttendaele, M., Moneim, A. A., Ceuppens, S. and Tahan, F. E. 2014. Microbiological safety of strawberries and lettuce for domestic consumption in Egypt. J. Food Process Technol., 5:308. doi: 10.4172/2157-7110.1000308. crossref(new window)

Ministry of Food and Drug Safety. 2010. Bacillus cereus (Risk Profile). pp. 3-4.

Santamaria, J. and Toranzos, G. A. 2003. Enteric pathogens and soil: a short review. Int. Microbiol., 6:5-9.

Barak, J. D. and Liang, A. S. 2008. Role of soil, crop debris, and a plant pathogen in Salmonella enteric contamination of tomato plants. PLoS One, 3(2):e1667. crossref(new window)