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Production of Phenyl Lactic Acid (PLA) by Lactic Acid Bacteria and its Antifungal Effect
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 Title & Authors
Production of Phenyl Lactic Acid (PLA) by Lactic Acid Bacteria and its Antifungal Effect
Song, June-Seob; Jang, Joo-Yeon; Han, Chang-Hoon; Yoon, Min-Ho;
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Phenyllactic acid (PLA) which is known as antimicrobial compound can be synthesized through the reduction of phenylpyruvic acid (PPA) by lactate dehydrogenase (LDH) of lactic acid bacteria (LAB). LAB producing PLA was isolated from Korea Kimchi and identified to Lactobacillus plantarum SJ21 by 16 rRNA gene sequence analysis. Cell-free supernatant (CFS) from L. plantarum SJ21 was assessed for both the capability to produce the antimicrobial compound PLA and the antifungal activity against four fungal pathogens (Rhizoctonia solani, Aspergillus oryzae, Botrytis cinerea, and Collectotricum aculatum). PLA concentration was investigated to be 3.23mM in CFS when L. plantarum SJ21 was grown in MRS broth containing 5mM PPA for 16 h. PLA production also could be promoted by the supplement of PPA and phenylalanine in MRS broth, but inhibited by the supplement of 4-hydroxyphenylpyruvic acid and tyrosine as precursors. Antifungal activity demonstrated that all fungal pathogens were sensitive to 5% CFS (v/v) of L. plantarum SJ21 with average growth inhibitions ranging from 27.32% to 69.05% (p<0.005), in which R. solani was the most sensitive to 69.05% and followed by B. cinerea, C. aculatum, and A. oryzae. The minimum inhibitory concentration (MIC) for commercial PLA was also investigated to show the same trend in the range from (2.11 mM) to (4.21 mM) at pH 4.0. The inhibition ability of CFS against the pathogens was not affected by heating or protease treatment. However, pH modification in CFS to 6.5 caused an extreme reduction in their antifungal activity. These results may indicate that antifungal activities in CFS were caused by acidic compounds like PLA or organic acids rather than proteins or peptides molecules.
Phenyllactic acid;Lactobacillus plantarum SJ21;Supplement;Antifungal effect;
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Dieuleveux, V., S. Lemarinier, and M. Gueguen. 1998. Antimicrobial spectrum and target site of D-3-phenyllactic acid. Int. J. Food Microbiol. 40:177-183. crossref(new window)

Dieuleveux, V. and M. Gueguen. 1998. Antimicrobial effects of D-3-phenyllactic acid on Listeria monocytogenes in TSB-YE medium, milk, and cheese. J. Food Prot. 61:1281-1285. crossref(new window)

Gerez, C.L., M.S. Carbajo, G. Rollán, G. Torres Leal, and G. Font de Valdez. 2010. Inhibition of citrus fungal pathogens by using lactic acid bacteria. J. Food Sci. 75(6):354-359. crossref(new window)

Gerez, C.L., M.J. Torres, G. Font de Valdez, and G. Rollan. 2013. Control of spoilage fungi by lactic acid bacteria. Biol. Control. 64:231-237. crossref(new window)

Hladikova, Z., J. Smetankova, G. Greif, and M. Greifkova. 2012. Antimicrobial activity of selected lactic acid cocci and production of organic acids. Acta Chim. Slov. 5:80-85.

Kumar, S., K. Tamura, I.B. Jakobsen, and M. Nei. 2001. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics. 17:1244-1245. crossref(new window)

Lavermicocca, P., F. Valerio, A. Evidente, S. Lazzaroni, A. Corsetti, and M. Gobbetti. 2000. Purification and characterization of novel antifungal compounds from the sourdough Lactobacillus plantarum strain 21B. Appl. Environ. Microbiol. 66:4084-4090. crossref(new window)

Li, X., B. Jiang, and B. Pan. 2007. Biotransformation of phenylpyruvic acid to phenyllactic acid by growing and resting cells of a Lactobacillus sp. Biotechnol. Lett. 29:593-597. crossref(new window)

Mu, W., C. Chen, X. Li, T. Zhang, and B. Jiang. 2009. Optimization of culture medium for the production of phenyllactic acid by Lactobacillus sp. SK007. Bioresource Technol. 100:1366-1370. crossref(new window)

Mu, W., Y. Yang, J. Jia, T. Zhang, and B. Jiang. 2010. Production of 4-hydroxyphenyllactic acid by Lactobacillus sp. SK007 fermentation. J. Biosci. Bioeng. 109:369-371. crossref(new window)

Mu, W., S. Yu, L. Zhu, B. Jiang, and T. Zhang. 2012. Production of 3-phenyllactic acid and 4-hydroxyphenyllactic acid by Pediococcus acidilactici DSM 20284 fermentation. Eur. Food. Res. Technol. 235:581-585. crossref(new window)

Prema, P., D. Smila, A. Palavesam, and G.Immanuel. 2010. Production and characterization of an antifungal compound (3-phenyllactic acid) produced by Lactobacillus plantarum strain. Food Bioprocess.Tech. 3:379-386. crossref(new window)

Rodriguez, N., J.M. Salgado, S. Cortes, and J.M. Dominguez. 2012. Antimicrobial activity of D-3-phenyllactic acid produced by fed-batch process against Salmonella enterica. Food Control. 25:274-284. crossref(new window)

Ryan, L.A.M., E. Zannini, F. Dal Bello, and A. Pawlowska. 2011. Lactobacillus amylovorus DSM 19280 as a novel food-grade antifungal agent for bakery products. Int. J. Food Microbiol. 146:276-283. crossref(new window)

Saitou, N., and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.

Schillinger, U., and J.V. Villareal, 2010. Inhibition of Penicillium nordicum in MRS medium by lactic acid bacteria isolated from foods. Food Control. 21:107-111. crossref(new window)

Schwenninger, S.M., C. Lacroix, S. Truttmann, C. Jans, C. Spördli, L. Bigler, and L. Meile. 2008. Characterization of low-molecular-weight antiyeast metabolites produced by a foodprotective Lactobacillus-Propionibacterium coculture. J. Food Prot. 71:2481-2487. crossref(new window)

Thompson, J.D., T.J. Gibson1, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882. crossref(new window)

Valerio, F., P. Lavermicocca, M. Pascale, and A. Visconti. 2004. Production of phenyllactic acid by lactic acid bacteria: an approach to the selection of strains contributing to food quality and preservation. FEMS Microbiol. Lett. 233:289-295. crossref(new window)

Vermeulen, N., M.G. Ganzle, and R.F. Vogel. 2006. Influence of peptide supply and cosubstrates on phenylalanine metabolism of Lactobacillus sanfranciscensis DSM20451T and Lactobacillus plantarum TMW1.468. J. Agri. Food Chem. 54:3832-3839. crossref(new window)

Wang, H., Y. Yan, J. Wang, H. Zhang, and W. Qi. 2012. Production and characterization of antifungal compounds produced by Lactobacillus plantarum IMAU0014. PLoS ONE. 7:e29452. crossref(new window)

Yvon, M., S. Thirouin, L. Rijnen, D. Fromentier, and J.C. Gripon. 1997. An aminotransferase from Lactococcus lactis initiates conversion of amino acids to cheese flavor compounds. Appl. Environ. Microbiol. 63:414-419.

Zheng, Z., C. Ma, C. Gao, F. Li, J. Qin, H. Zhang, K. Wang, and P. Xu. 2011. Efficient conversion of phenylpyruvic acid to phenyllactic acid by using whole cells of Bacillus coagulans SDM. PLoS ONE. 6(4):e19030. crossref(new window)