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Assessment of Soil Microbial Communities in Carotenoid-Biofortified Rice Ecosystem
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 Title & Authors
Assessment of Soil Microbial Communities in Carotenoid-Biofortified Rice Ecosystem
Sohn, Soo-In; Oh, Young-Ju; Kim, Byung-Yong; Lee, Bumkyu; Lee, Si-Myung; Oh, Sung-Dug; Lee, Gang-Seob; Yun, Doh-Won; Cho, Hyun-Suk;
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 Abstract
This study was conducted to investigate the effect of Psy-2A-CrtI (PAC), a genetically modified (GM) rice with enhanced -carotene, on the soil microbial community. The soil used to cultivate GM rice and its wild-type, Nakdong, was analyzed for population density, denaturing gradient gel electrophoresis (DGGE), and pyrosequencing. It was found that the bacterial, fungal and actinomycetes population densities of the PAC soils were within the range of those of the non-GM rice cultivar, Nakdong. The DGGE banding patterns of the GM and non-GM soils were also similar, suggesting that the bacterial community structures were stable within a given month and were unaffected by the presence of a GM plant. The pyrosequencing result showed a temporal difference in microorganism taxon and distribution ratio, but no significant difference between GM and non-GM was found. The persistence of the transgene DNA in the plant and surrounding soil were investigated for different time periods. There were differences in the persistence within the plant depending on the gene, but they could not be detected after 5 weeks. Also the transgenes were not detected in the surrounding soil. These results indicate that soil microbial communities are unaffected by the cultivation of a PAC rice within the experimental time frame.
 Keywords
Genetically modified rice;Soil microbial community;DNA persistence;
 Language
English
 Cited by
 References
1.
Ahn, J.H., M.C. Kim, H.C. Shin, M.K. Choi, S.S. Yoon, T.S. Kim, H.G. Song, G.H. Lee, and J.O. Ka. 2006. Improvement of PCR amplification bias for community structure analysis of soil bacteria by denaturing gradient gel electrophoresis. J. Microbiol. Biotechnol. 16:1561-1569.

2.
Bardgett, R.D., C.S. Dentin, and R. Cook. 1999. Below-ground herbivory promotes soil nutrient transfer and root growth in grassland. Ecol. Lett. 2:357-360. crossref(new window)

3.
Barriuso, J., J.R. Valverde, and R.P. Mellado. 2012. Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period. PLoS ONE 7:e35481. crossref(new window)

4.
Bertolla, S. and P. Simonet. 1999. Horizontal gene transfer in environment: natural transformation as a putative process for gene transfers between transgenic plants and microorganisms. Res. Microbiol. 150:375-384. crossref(new window)

5.
Cruz-Martinez, K., A. Rosling, Y. Zhang, M. Song, G.L. Anderson, and J.F. Banfield. 2012. Effect of rainfall-induced soil geochemistry dynamics on grassland soil microbial communities. Appl. Environ. Microbiol. 78:7587-7595. crossref(new window)

6.
Donegan, K.K., C.J. Palm, V.J. Fieland, L.A. Porteous, L.M. Ganio, D.L. Schaller, L.Q. Bucao, and R.J. Seidler. 1995. Changes in levels, species, and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin. Appl. Soil Ecol. 2:111-124. crossref(new window)

7.
Gebhard, F. and K. Smalla. 1998. Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA. Appl. Environ. Microbiol. 4:1550-1554.

8.
Ha, S.W., Y.S. Liang, H. Jung, M.J. Ahn, S.C. Suh, S.J. Kweon, D.H. Kim, Y.M. Kim, and J.K. Kim. 2010. Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm. Plant Biotechnol. J. 8:928-938. crossref(new window)

9.
Gebhard, F. and K. Smalla. 1998. Transformation of Acinetobacter sp. Strain BD413 by transgenic sugar beet DNA. Appl. Environ. Microb. 64:1550-1554.

10.
Hay, I., M.-J. Morency, and A. Seguin. 2002. Assessing the persistence of DNA in decomposing leaves of genetically modified poplar trees. Can. J. For. Res. 32:977-982. crossref(new window)

11.
Hobbie, S.E., P.B. Reich, J. Oleksyn, M. Ogdahl, R. Zytkowiak, C. Hale, and P. Karolewski. 2006. Tree species effects on decomposition and forest floor dynamics in a common garden. Ecology 87:2288-2297. crossref(new window)

12.
Hur, M., Y. Kim, H.R. Song, J.M. Kim, Y.I. Choi, and H.N. Yi. 2011. Effect of genetically modified poplars on soil microbial communities during the phytoremediation of waste mine tailings. Appl. Environ. Microbiol. 77:7611-7619. crossref(new window)

13.
Icoz, I., D. Saxena, D.A. Andow, C. Zwahlen, and G. Stotzky. 2008. Microbial populations and enzyme activities in soil in situ under transgenic corn expressing cry proteins from Bacillus thuringiensis. J. Environ. Qual. 37:647-662. crossref(new window)

14.
James, C. 2013. Global status of commercialized biotech/GM crops: 2013. ISAAA Briefs No. 45, Ithaka, NY.

15.
Jung, B.G., G.H. Jo, E.S. Yun, J.H. Yoon, and Y.H. Kim, 1998. Monitoring on chemical properties of bench marked paddy soils in Korea. Korean J. Soil Sci. Fert. 31:246-252.

16.
Lee, S.H., C.G. Kim, and H. Kang. 2011. Temporal dynamics of bacterial and fungal communities in a genetically modified (GM) rice ecosystem. Microb. Ecol. 61:646-659. crossref(new window)

17.
Lee, B., C.G. Kim, J.Y. Park, K.W. Park, H. Yi, C.H. Harn, H.M. Kim. 2007. Assessment of the persistence of DNA in decomposing leaves of CMVP0-CP transgenic chili pepper in the field conditions. Kor. J. Environ. Agric. 26: 319-324. crossref(new window)

18.
Liu, F.X., Z.P. Xu, J.H. Chang, J. Meng, F.X. Zhu, Y.C. Shen, and J.L. Shen. 2008. Resistance allele frequency to Bt cotton in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) in China. J. Econ. Entomol. 101: 933-943. crossref(new window)

19.
Lynch J. 1994. The rhizosphere-form and function. Appl. Soil Ecol. 1:193-198. crossref(new window)

20.
NIAST. 2000. Methods of Soil Chemical Analysis. National Institute of Agricultural Science and Technology, RDA, Suwon, Korea.

21.
Oger, P., A. Petit, and Y. Dessaux. 1997. Genetically engineered plants producing opines alter their biological environment. Nat. Biotechnol. 15:369-372. crossref(new window)

22.
Oger, P., H. Mansouri, and Y. Dessaux. 2000. Effect of crop rotation and soil cover on alteration of the soil microflora generated by the culture of transgenic plants producing opines. Mol. Ecol. 9:881-890. crossref(new window)

23.
Robertson, B.H., M.J. Grubman, G.N. Weddell, D.M. Moore, J.D. Welsh, T. Fisher, D.J. Dowbenko, D.G. Yansura, B. Small, and D.G. Kleid. 1985. Nucleotide and amino acid sequence coding for polypeptides of foot-and-mouth disease virus type A12. J. Virol. 54:651-660.

24.
Saxena, D. and G. Stotzky. 2001b. Bacillus thuringiensis (Bt) toxin released from root exudates and biomass of Bt corn has no apparent effect on earthworms, nematodes, protozoa, bacteria, and fungi in soil. Soil Biol. Biochem. 33:1225-1230. crossref(new window)

25.
Wei, M., F. Tan, H. Zhu, K. Cheng, X. Wu, J. Wang, K. Zhao, and X. Tang. 2012. Impact of Bt-transgenic rice (SHK601) on soil ecosystems in the rhizosphere during crop development. Plant Soil Environ. 58:217-223.

26.
Widmer, F., Seidler, R.J., Donegan, K.K., Reed, G.L. 1997. Quantification of transgenic plant marker gene persistence in the field. Mol. Ecol. 6:1-7. crossref(new window)