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The Effects of Phosphate Starvation on the Activities of Acid and Alkaline Phosphatase, Fructose-1,6-bisphosphatase, Sucrose-phosphate Synthase and Nitrate Reductase in Melon (Cucumis melo L.) Seedlings
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 Title & Authors
The Effects of Phosphate Starvation on the Activities of Acid and Alkaline Phosphatase, Fructose-1,6-bisphosphatase, Sucrose-phosphate Synthase and Nitrate Reductase in Melon (Cucumis melo L.) Seedlings
Kang, Sang-Jae; Lee, Chang-Hee; Park, Man;
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Plants response to phosphate starvation include the changes of activity of some enzymes, such as phosphatases, fructose-1,6-bisphosphatase, sucrose-phosphate synthase and nitrate reductase. In this study, to determine the effects of phosphate starvation on the change of activities of acid and alkaline phosphatase, fructose-1,6-bisphosphatase, sucrose-phosphate synthase, and nitrate reductase were studied in melon seedlings (Cucumis melo L.). The content of the protein and chlorophyll tended to relatively reduced in melon seedlings subjected to phosphate starvation. Acid phosphatase activity in first and second leaves of melon seedlings was relatively higher than that of third and fourth leaves of seedlings in 14 days after phosphate starvation treatment, respectively. Active native-PAGE band patterns of acid phosphatase in melon leaves showed similar to activities of acid phosphatase, whereas alkaline phosphatase activity was different from the change in the activity of acid phosphatase. Inorganic phosphate content in melon seedlings leaves was constant. The changes of Fructose-1,6-bisphosphatase and sucrose phosphate synthase activities showed similar patterns in melon seedlings leaves, and between these enzymes activities and phosphate nutrition negatively related. Fructose-1,6- bisphosphatase and sucrose phosphate synthase activities showed significant difference in second and fourth leaves, but nitrate reductase showed significant difference in first and second leaves in 14days after phosphate starvation treatment. We concluded that phosphate nutrition could affect the distribution of phosphate, carbon and nitrogen in melon seedlings.
Inorganic phosphate;Phosphatases;Phosphate starvation;Melon seedling;
 Cited by
Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts: polyphenol oxidase in Beta vulgaris. Plant Physiol. 24:1-15. crossref(new window)

Ascencio, J. 1997. Root secreted acid phosphatase kinetics as a physiological marker for phosphorus deficiency. J Plant Nutr. 20:9-26. crossref(new window)

Bariola, P.A., C.J. Howard, C.B. Taylor, M.T. Verburg, V.D. Jaglan, and P.J. Green. 1994. The Arabidopsis ribonuclease gene RNA1 is tightly controlled in response to phosphate limitation. Plant J. 6:673-685. crossref(new window)

Barrette-Lennard, E.G., A.D. Robson, and H. Greenway. 1982. Effect of phosphorus deficiency and water deficit on phosphatase activities from wheat leaves. J. Exp. Bot. 33:682-693. crossref(new window)

Beck, E. and P. Ziegler. 1989. Biosynthesis and degradation of starch in higher plants. Annu. Rev. Plant Physio. Plant Mol. Biol. 40:95-118. crossref(new window)

Bozzo, G.G., K.G. Raghothama, and W.C. Plaxton. 2004. Structural ad kinetic properties of a novel purple acid phosphatase from phosphate-starved tomato (Lycopersicon esculentum) cell culture. Biochem. J 377:419-428. crossref(new window)

Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microquantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254. crossref(new window)

Drueckes, P., R. Schinzel, and D. Palm. 1995. Photometric microtiter assay of inorganic phosphate in the presence of acid-labile organic phosphate. Anal. Biochem. 230:173-177. crossref(new window)

Duff, S.M.G., G. Sarah, and W.C. Plaxton. 1994. The roles of acid phosphatases in plant phosphorus metabolism. Physiol. Plant 90:791-800. crossref(new window)

Duff, S.M.G., D.D. Lefebvre and W.C. Plaxton. 1991. Phosphate starvation response in plant cells: De novo synthesis and degradation of acid phosphatase. Proc. Natl. Acad. Sci. USA 88:9538-9542. crossref(new window)

Epstein, E., and A.J. Bloom. 2005. Ecology and Environmental Stress. p. 328-330. Mineral Nutrition of Plants : Principles and Perspective, 2 nd edition, Sinauer Associate, MA, USA.

Granjeiro, P.A., C.V. Verissima, J.M. Granjeiro, E.M. Taga, and H. Aoyama. 1999. Purification and kinetic properties of a caster bean seed acid phosphatase containing sulfhydryl groups. Physiol. Plant 107:151-158. crossref(new window)

Huber, J.L.A., Huber, S.C., and T.H. Nielsen. 1989. Protein phosphorylatiojn as a mechanism for regulation of spinach leaf sucrose-phosphate synthase activity. Arch Biochem. Biophys. 270:681-690 crossref(new window)

Jeschke, W.D., E.A. Kirkby, A.D. Peuke, J.S. Pate, and W. Hartung. 1997. Effects of P deficiency on assimilation and transport of nitrate and phosphate in intact plants of caster bean (Ricinus communis L.). J. Exp. Bot. 48:75-91. crossref(new window)

Kerr, P.S., S.C. Huber, and D.W. Israel. 1984. Effect of N-source on soybena leaf sucrose phosphate synthase, starch formation and whole plant growth. Plant Physiol. 75:483-488. crossref(new window)

Lefebvre, D.D., S.M.G. Duff, C.A. Fife, C. Julien-Inalsingh, and W.C. Plaxton. 1990. response to phosphate deprivation in Brassica nigra suspension cells. Plant Physiol. 93:504-511. crossref(new window)

Li, D., H. Zhu, K. Liu, X. Liu, G. Leggewie, M. Udvardi, and D. Wang. 2002. Purple acid phosphatase of Arabidopsis thaliana. J. Biol. Chem. 277:2772-27781.

Lichtenthaler, H. K. 1987. Chlorophyll and carotenoids: pigment of photosynthetic biomembranes. Methods in Enzymol. 140:350-382.

Lin, W-Y., S. Lin, and T-J. Chiou. 2009. Molecular regulators of phosphate homeostasis in plants. J. Exp. Bot. 60:1427-1438. crossref(new window)

Liu, C., U.S. Muchhal, M. Uthappa, A.K. Kononowicz, and K.G. Raghothama. 1998. Tomato phosphate transporter genes are differentially regulated in plant tissues by phosphorus. Plant Physiol. 116:91-99. crossref(new window)

McMichael, R.W., Bachmann, M., and S.C. Huber. 1995. Spinach leaf sucrose -phosphate synthase and nitrate reductase are phosphorylated/inactivated by multiple protein kinase in vitro. Plant Physiol. 108:1077-1082. crossref(new window)

Miller, S.S., J. Liu, D.L. Allan, C.J. Menzhuber, M. Fedorova, and C.P. Vance. 2001. Molecular control of acid phosphatase secretion intgo the rhizosphere of proteiod roots from phosphorus-stressed white lupin. Plant Physiol. 127:594-606. crossref(new window)

Muchhal, U.S., J.M. Pardo, and K.G. Raghathama. 1996. Phosphate transporters from the higher plant Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 93:10519-10523. crossref(new window)

NIAST. 1988. Methods of soil chemical analysis. National Institute of Agricultural Science and Technology, RDA, Suwon, Korea.

Pan, S. (1987) Characterization of multiple acid phosphatases in salt stressed spinach leaves. Aust. J Plant Physiol. 14:117-124. crossref(new window)

Robinson, W.D., J. Park, H.T. Tran, H.A. Del Vecchio, S. Ying, J.L. Zins, K. Patel, T.D. McKnight, and W.C. Plaxton. 2012. The secreted purple acid phosphatase isozymes AtPAP12 and AtPAP26 play a pivotal role in extracellular phosphate scavenging by Arabidopsis thaliana. J. Exp. Bot. 63:6531-6542. crossref(new window)

Rufty, T.W., P.S. Kerr, and S.C. Huber. 1983. Characterization of diurnal changes in activities of enzymes involved in sucrose biosynthesis. Plant Physiol. 73:428-433. crossref(new window)

Solomon, L.P., and M.J. Barber. 1990. Assimilatory nitrate reductase: functional properties and regulation. Annu. Rev. Plant Biol. 41:224-253.

Taiz, L., and E. Zeiger. 2002. Photosynthesis: carbon reaction. In: Plant Physiology, 3rd edition, Sinauer Associates, Massachusettes, p. 145-170.

Trull, M.C., M.J. Guitman, J.P. Lynch, and J. Deikman. 1997. The response wild type and ABA-mutant Arabidopsis thaliana plants to phosphorus deficiency. Plant Cell Environ. 20:85-92. crossref(new window)