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Evidence of hydrolyzed traditional Korean red ginseng by malted barley on activation of receptor interacting proteins 2 and IkappaB kinase-beta in mouse peritoneal macrophages
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  • Journal title : TANG [HUMANITAS MEDICINE]
  • Volume 2, Issue 3,  2012, pp.27.1-27.6
  • Publisher : Association of Humanitas Medicine
  • DOI : 10.5667/tang.2012.0020
 Title & Authors
Evidence of hydrolyzed traditional Korean red ginseng by malted barley on activation of receptor interacting proteins 2 and IkappaB kinase-beta in mouse peritoneal macrophages
Rim, Hong-Kun; Kim, Kyu-Yeob; Moon, Phil-Dong;
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Red ginseng, which has a variety of biological and pharmacological activities including antioxidant, anti-inflammatory, antimutagenic and anticarcinogenic effects, has been used for thousands of years as a general tonic in traditional oriental medicine. Here, we tested the immune regulatory activities of hydrolyzed red ginseng by malted barley (HRG) on the expressions of receptor interacting proteins (Rip) 2 and kinase-beta (IKK-) in mouse peritoneal macrophages. We show that HRG increased the activations of Rip 2 and IKK- for the first time. When HRG was used in combination with recombinant interferon- (rIFN-), there was a marked cooperative induction of nitric oxide (NO) production. The increased expression of inducible NO synthase from rIFN- plus HRG-stimulated cells was almost completely inhibited by pre-treatment with pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor- (NF-). In addition, the treatment of peritoneal macrophages with rIFN- plus HRG caused significant increases in tumor necrosis factor (TNF)- mRNA expression and production. Because NO and TNF- play an important role in the immune function and host defense, HRG treatment can modulate several aspects of the host defense mechanisms as a result of the stimulations of the inducible nitric oxide synthase and NF-. In conclusion, our findings demonstrate that HRG increases the productions of NO and TNF- from rIFN--primed macrophages and suggest that Rip2/IKK- plays a critical role in mediating these immune regulatory effects of HRG.
red ginseng;receptor interacting proteins 2; kinase-beta;nuclear factor-;tumor necrosis factor-;nitric oxide;
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