Advanced SearchSearch Tips
Anticholinesterase activity of Cinnamomum zeylanicum L. leaf extract
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : TANG [HUMANITAS MEDICINE]
  • Volume 4, Issue 2,  2014, pp.11.1-11.6
  • Publisher : Association of Humanitas Medicine
  • DOI : 10.5667/tang.2013.0034
 Title & Authors
Anticholinesterase activity of Cinnamomum zeylanicum L. leaf extract
Dalai, Manoj Kumar; Bhadra, Santanu; Chaudhary, Sushil Kumar; Chanda, Joydeb; Bandyopadhyay, Arun; Mukherjee, Pulok K.;
  PDF(new window)
Cinnamomum zeylanicum (C. zeylanicum) is a tropical evergreen tree of Lauraceae family. It is one of the oldest culinary spices known and used traditionally in many cultures for centuries. In addition to its culinary uses, cinnamon also possesses as a folk remedy of many health disease condition including analgesic, antiseptic, antispasmodic, aphrodisiac, astringent, carminative, haemostatic, insecticidal, and parasiticide and memory enhancing property. This study was aimed to assess the acetylcholinesterase and butyrylcholinesterase inhibitory activity of standardized methanol extract of the C. zeylanicum. Gas chromatography - mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analysis were done to identify the presence of eugenol as chemical component and support the neuroprotective activity in the extract. Anticholinesterase inhibitory activity of crude methanol extract of C. zeylanicum leaves and cinnamon oil were evaluated by 96-well microtiter plate assay and thin layer chromatography bioassay detection methods. This study revealed that cinnamon oil () has better anticholinesterase activity than methanol extract (). In HPLC analysis, retention time of eugenol in cinnamon oil was found to be 15.81 min which was comparable with the retention time (15.99 min) of the reference standard, eugenol. Seven chemical compounds were identified by GC-MS analysis, in which eugenol as an important phytoconstituents. Thus the phytochemicals from C. zeylanicum methanol leaves extract could be developed as potential source of anticholinesterase activity, with particular benefit in the symptomatic treatment of Alzheimer`s disease.
Cinnamomum zeylanicum;anticholinesterase;Alzheimer`s disease;eugenol;gas chromatography - mass sepctrometry;high performance liquid chromatography;
 Cited by
Antiamylase, Anticholinesterases, Antiglycation, and Glycation Reversing Potential of Bark and Leaf of Ceylon Cinnamon (Cinnamomum zeylanicum Blume) In Vitro, Evidence-Based Complementary and Alternative Medicine, 2017, 2017, 1  crossref(new windwow)
Adams RP. Identification of Essential Oils by Ion Trap Mass Spectroscopy. (San Diego, USA: Academic Press), 2001.

Bhadra S, Mukherjee PK, Bandyopadhyay A. Cholinesterase inhibition activity of Marsilea quadrifolia Linn. an edible leafy vegetable from West Bengal, India. Nat Prod Res. 2012;26:1519-1522. crossref(new window)

Bhadra S, Mukherjee PK, Kumar NS, Bandyopadhyay A. Anticholinesterase activity of standardized extract of Illicium verum Hook.f. fruits. Fitoterapia. 2011;82:342-346. crossref(new window)

Boga M, Hacibekiroglu I, Kolak U. Antioxidant and anticholinesterase activities of eleven edible plants. Pharm Biol. 2011;49:290-295. crossref(new window)

Chaudhary SK, Maity N, Nema NK, Bhadra S, Saha BP, Mukherjee PK. Angiotensin converting enzyme inhibition activity of fennel and coriander oils from India. Nat Prod Comm. 2013;8:671-672.

Ellman GL, Lourtney DK, Andres V, Gmelin G. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharm.1961;7:88-95. crossref(new window)

Gholamhoseinian A, Moradi MN, Sharifi-far F. Screening the methanol extracts of some Iranian plants for acetylcholinesterase inhibitory activity. Res Pharm Sci. 2009;4:105-112.

Jaganathan SK, Supriyanto E. Antiproliferative and molecular mechanism of eugenol-induced apoptosis in cancer cells. Molecules. 2012;17:6290-6304. crossref(new window)

Kermasha S, Goetghebeur M, Dumont J. Separation and Characterization of Pepper-Contaminated cinnamon Using HPLC Analyses. J Food Sci Tech. 1994;27:578-582.

Kovats E. Gas chromatographic characterization of organic substances in the retention index system. (New York, USA: Marcel Dekker), 1965.

Kumar NS, Mukherjee PK, Bhadra S, Saha BP, Pal BC. Acetylcholinesterase inhibitory potential of a carbazole alkaloid, Mahanimbine from Murraya koenigii. Phytother Res. 2010;24:629-631.

Kumar P, Singh VK, Singh DK. Kinetics of enzyme inhibition by active molluscicidal agents ferulic acid, umbelliferone, eugenol and limonene in the nervous tissue of snail Lymnaea acuminate. Phytother Res. 2009;23:172-177. crossref(new window)

Laekeman G. Assessment report on Cinnamomum verum J. S. Presl, cortex and corticisaetheroleum. (London, UK: European Medicines Agency), 2011.

Mallavarapu GR, Ramesh S, Chandrasekhara RS, Rajeswara Rao BR, Kaul PN, Bhattacharya AK. Investigation of the essential oil of cinnamon leaf grown at Bangalore and Hyderabad. Flavour Frag J. 1995;10:239-242. crossref(new window)

Mateos-Martin ML, Perez-Jimenez J, Fuguet E, Torres JL. Profile of urinary and fecal proanthocyanidin metabolites from common cinnamon (Cinnamomum zeylanicum L.) in rats. Mol Nutr Food Res. 2012;56:671-675. crossref(new window)

Mukherjee PK, Ahamed KF, Kumar V, Mukherjee K, Houghton PJ. Protective effect of biflavones from Araucaria bidwillii Hook in rat cerebral ischemia/reperfusion induced oxidative stress. Behav Brain Res. 2007b;178:221-228. crossref(new window)

Mukherjee PK, Houghton PJ. The worldwide phenomenon of increased use of herbal products: Opportunities and threats. In Evaluation of Herbal Medicinal Products, Mukherjee PK, Houghton PJ ed. (London, UK: Pharmaceutical Press), pp. 3-12, 2009.

Mukherjee PK, Kumar V, Houghton PJ. Screening of Indian medicinal plants for acetylcholinesterase inhibitory activity. Phytother Res. 2007a;21:1142-1145. crossref(new window)

Nyadjeu P, Dongmo A, Nguelefack TB, Kamanyi A. Antihypertensive and vasorelaxant effects of Cinnamomum zeylanicum stem bark aqueous extract in rats. J Complement Integr Med. 2011;8.

O'Shea SK, Von Riesen DD, Rossi LL. Isolation and analysis of essential oils from spices. J Chem Educ. 2012;89:665-668. crossref(new window)

Prasad SN, Muralidhara. Neuroprotective Efficacy of Eugenol and Isoeugenol in Acrylamide-Induced Neuropathy in rats: Behavioral and Biochemical evidence. Neurochem Res. 2013;38:330-345. crossref(new window)

Rana VS, Langoljam RD, Verdeguer M, Blazquez MA. Chemical variability in the essential oil of Cinnamomum tamala L. Leaves from India. Nat Prod Res. 2012;26:1355-1357. crossref(new window)

Ranasinghe P, Pigera, S, Premakumara GAS, Galappaththy P, Constantine GR, Katulanda P. Medicinal properties of 'true' cinnamon (Cinnamomum zeylanicum): a systematic review. BMC Complement Altern Med. 2013;13:275-284. crossref(new window)

Ross MSF. Analysis of cinnamon oils by high-pressure liquid chromatography. J Chromatogr. 1976:118:273-275. crossref(new window)

Trajano VN, Lima EO, Travassos AE, de Souza EL. Inhibitory effect of the essential oil from Cinnamomum zeylanicum Blume leaves on some food-related bacteria. Food Sci Tech. 2010;30:771-775. crossref(new window)

White P, Hiley CR, Goodhardt MJ, Carrasco LH, Keet JP, Williams IE, Bowen DM. Neocortical cholinergic neurons in elderly people. Lancet. 1977;309:668-671. crossref(new window)

Yang CY, Lv ZP, Zheng CG. Efficacy and reliability of huperzine A in mild and moderate Alzheimer's disease. Chin J Clin Rehabil. 2003;7:4258-4259.