Advanced SearchSearch Tips
Effect of KH-BaRoKer-SeongJangTang based on traditional medicine theory on longitudinal bone growth
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : TANG [HUMANITAS MEDICINE]
  • Volume 4, Issue 2,  2014, pp.14.1-14.6
  • Publisher : Association of Humanitas Medicine
  • DOI : 10.5667/tang.2014.0020
 Title & Authors
Effect of KH-BaRoKer-SeongJangTang based on traditional medicine theory on longitudinal bone growth
Kim, Min-Ho; Jeong, Hyeonseok; Park, Myungduek; Moon, Phil-Dong;
  PDF(new window)
KH-BaRoKer-SeongJangTang (KBS) is a recently developed formulation by using traditional drugs considering traditional medical theory of Oriental books such as ShinNongBonChoGyeong and JuRye, which has been used to improve the growth of child in Korea. Although KBS is usually prescribed to many children who are in retard for their age, its pharmacological effects have not been fully understood in experimental models. The aim of this study was to evaluate the effects of KBS on bone growth. Growth plate thickness and bone parameters such as bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), connection density (Conn.D), and total porosity were analyzed by means of microcomputed tomography. Serum insulin-like growth factor-I (IGF-I) levels were measured by enzyme-linked immunosorbent assay. Hepatic IGF-I mRNA expression was analyzed by real-time polymerase chain reaction. Phosphorylation of signal transducer and activator of transcription5 (STAT5) was investigated using Western blot analysis and immunohistochemistry. The thickness of growth plate was increased by KBS. BV/TV, Tb.Th, TbN, Conn.D, and total porosity were improved by KBS. Hepatic IGF-I mRNA and serum IGF-I levels were elevated by KBS. Phosphorylation of STAT5 was increased with administration of KBS. These results suggest that KBS would be helpful to children who are in retard for their age through the elevation of IGF-I.
growth plate;bone parameters;insulin-like growth factor-I;signal transducer and activator of transcription5;
 Cited by
Protective effect of porcine placenta in a menopausal ovariectomized mouse, Reproduction, 2015, 150, 3, 173  crossref(new windwow)
Abad V, Meyers JL, Weise M, Gafni RI, Barnes KM, Nilsson O, Bacher JD, Baron J. The role of the resting zone in growth plate chondrogenesis. Endocrinology. 2002;143:1851-1857. crossref(new window)

Barnes PJ. How corticosteroids control inflammation: Quintiles Prize Lecture 2005. Br J Pharmacol. 2006;148:245-254.

Baron J, Huang Z, Oerter KE, Bacher JD, Cutler GB Jr. Dexamethasone acts locally to inhibit longitudinal bone growth in rabbits. Am J Physiol. 1992;263:E489-E492.

Blaser C, Wittwer M, Grandgirard D, Leib SL. Adjunctive dexamethasone affects the expression of genes related to inflammation, neurogenesis and apoptosis in infant rat pneumococcal meningitis. PLoS One. 2011;6:e17840. crossref(new window)

Brooks AJ, Waters MJ. The growth hormone receptor: mechanism of activation and clinical implications. Nat Rev Endocrinol. 2010;6:515-525. crossref(new window)

Brynskov T, Laugesen CS, Halborg J, Kemp H, Sorensen TL. Longstanding refractory pseudophakic cystoid macular edema resolved using intravitreal 0.7 mg dexamethasone implants. Clin Ophthalmol. 2013;7:1171-1174.

Chae HJ, Choi KH, Chae SW, Kim HM, Shin TK, Lee GY, Jeong GS, Park HR, Choi HI, Kim SB, Yoo SK, Kim HR. Placenta hominis protects osteoporosis in ovariectomized rats. Immunopharmacol Immunotoxicol. 2006;28:165-173. crossref(new window)

Choi MJ, Chang KJ. Effect of dietary taurine and arginine supplementation on bone mineral density in growing female rats. Adv Exp Med Biol. 2013;776:335-345. crossref(new window)

Chrysis D, Ritzen EM, Savendahl L. Growth retardation induced by dexamethasone is associated with increased apoptosis of the growth plate chondrocytes. J Endocrinol. 2003;176:331-337. crossref(new window)

Chrysis D, Zaman F, Chagin AS, Takigawa M, Savendahl L. Dexamethasone induces apoptosis in proliferative chondrocytes through activation of caspases and suppression of the Aktphosphatidylinositol 3'-kinase signaling pathway. Endocrinology. 2005;146:1391-1397. crossref(new window)

Davey HW, Park SH, Grattan DR, McLachlan MJ, Waxman DJ. STAT5b-deficient mice are growth hormone pulse-resistant. Role of STAT5b in sex-specific liver p450 expression. J Biol Chem. 1999;274:35331-35336. crossref(new window)

de Vos AM, Ultsch M, Kossiakoff AA. Human growth hormone and extracellular domain of its receptor: crystal structure of the complex. Science. 1992:255:306-312. crossref(new window)

Fock RA, Rogero MM, Vinolo MA, Curi R, Borges MC, Borelli P. Effects of protein-energy malnutrition on NF-kappaB signalling in murine peritoneal macrophages. Inflammation. 2010;33:101-109. crossref(new window)

Giuliano C, Smalligan RD, Mitchon G, Chua M. Role of dexamethasone in the prevention of migraine recurrence in the acute care setting: a review. Postgrad Med. 2012;124:110-115.

Han NR, Kim HM, Jeong HJ. Kanamycin activates caspase-1 in NC/Nga mice. Exp Dermatol. 2011;20:659-663. crossref(new window)

Harris L, Senagore P, Young VB, McCabe LR. Inflammatory bowel disease causes reversible suppression of osteoblast and chondrocyte function in mice. Am J Physiol Gastrointest Liver Physiol. 2009;296:G1020-G1029. crossref(new window)

Kim HJ, Bae YC, Park RW, Choi SW, Cho SH, Choi YS, Lee WJ. Bone-protecting effect of safflower seeds in ovariectomized rats. Calcif Tissue Int. 2002;71:88-94. crossref(new window)

King B, Jiang Y, Su X, Xu J, Xie L, Standard J, Wang W. Weight control, endocrine hormones and cancer prevention. Exp Biol Med. 2013;238:502-508. crossref(new window)

Kugelberg M, Shafiei K, Ohlsson C, Savendahl L, Zetterstrom C. Glucocorticoid eye drops inhibit growth in the newborn rabbit. Acta Paediatr. 2005;94:1096-1101. crossref(new window)

Le Roith D, Bondy C, Yakar S, Liu JL, Butler A. The somatomedin hypothesis: 2001. Endocr Rev. 2001;22:53-74. crossref(new window)

Lee YS, Choi CW, Kim JJ, Ganapathi A, Udayakumar R, Kim SC. Determination of mineral content in methanolic safflower (Carthamus tinctorius L.) seed extract and its effect on osteoblast markers. Int J Mol Sci. 2009;10:292-305. crossref(new window)

Lui JC, Baron J. Effects of glucocorticoids on the growth plate. Endocr Dev. 2011;20:187-193.

Moon PD, Choi IH, Kim HM. Epigallocatechin-3-O-gallate inhibits the production of thymic stromal lymphopoietin by the blockade of caspase-1/NF-${\kappa}B$ pathway in mast cells. Amino Acids. 2012;42:2513-2519. crossref(new window)

Moon PD, Kim HM. Thymic stromal lymphopoietin is expressed and produced by caspase-1/NF-${\kappa}B$ pathway in mast cells. Cytokine. 2011;54:239-243. crossref(new window)

Moon PD, Kim HM. Suppression of thymic stromal lymphopoietin production by rutin in mast cells. Food Chem. 2012;133:76-81. crossref(new window)

Ngueguim FT, Khan MP, Donfack JH, Siddiqui JA, Tewari D, Nagar GK, Tiwari SC, Theophile D, Maurya R, Chattopadhyay N. Evaluation of Cameroonian plants towards experimental bone regeneration. J Ethnopharmacol. 2012;141:331-337. crossref(new window)

Pennisi P, Clementi G, Prato A, Luca T, Martinez G, Mangiafico RA, Pulvirenti I, Muratore F, Fiore CE. L-arginine supplementation normalizes bone turnover and preserves bone mass in streptozotocin-induced diabetic rats. J Endocrinol Invest. 2009;32:546-551. crossref(new window)

Puignero V, Salgado J, Queralt J. Effects of cyclosporine and dexamethasone on IgE antibody response in mice, and on passive cutaneous anaphylaxis in the rat. Int Arch Allergy Immunol. 1995;108:142-147. crossref(new window)

Rabkin R, Sun DF, Chen Y, Tan J, Schaefer F. Growth hormone resistance in uremia, a role for impaired JAK/STAT signaling. Pediatr Nephrol. 2005;20:313-318. crossref(new window)

Rahman A, Rizwan S, Waycaster C, Wall GM. Pooled analysis of two clinical trials comparing the clinical outcomes of topical ciprofloxacin/dexamethasone otic suspension and polymyxin B/neomycin/hydrocortisone otic suspension for the treatment of acute otitis externa in adults and children. Clin Ther. 2007;29:1950-1956. crossref(new window)

Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993;123:1939-1951.

Sharan K, Mishra JS, Swarnkar G, Siddiqui JA, Khan K, Kumari R, Rawat P, Maurya R, Sanyal S, Chattopadhyay N. A novel quercetin analogue from a medicinal plant promotes peak bone mass achievement and bone healing after injury and exerts an anabolic effect on osteoporotic bone: the role of aryl hydrocarbon receptor as a mediator of osteogenic action. J Bone Miner Res. 2011;26:2096-2111. crossref(new window)

Soheilian M, Ramezani A, Azimzadeh A, Sadoughi MM, Dehghan MH, Shahghadami R, Yaseri M, Peyman GA. Randomized trial of intravitreal clindamycin and dexamethasone versus pyrimethamine, sulfadiazine, and prednisolone in treatment of ocular toxoplasmosis. Ophthalmology. 2011;118:134-141. crossref(new window)

Thompson BT. Glucocorticoids and acute lung injury. Critl Care Med. 2003;31:S253-S257. crossref(new window)

Weng CY, Kothary PC, Verkade AJ, Reed DM, Del Monte MA. MAP kinase pathway is involved in IGF-1-stimulated proliferation of human retinal pigment epithelial cells (hRPE). Curr Eye Res. 2009;34:867-876. crossref(new window)

Wershil BK, Furuta GT, Lavigne JA, Choudhury AR, Wang ZS, Galli SJ. Dexamethasone or cyclosporin A suppress mast cell-leukocyte cytokine cascades. Multiple mechanisms of inhibition of IgE- and mast cell-dependent cutaneous inflammation in the mouse. J Immunol. 1995;154:1391-1398.

Woelfle J, Chia DJ, Rotwein P. Mechanisms of growth hormone (GH) action. Identification of conserved Stat5 binding sites that mediate GH-induced insulin-like growth factor-I gene activation. J Biol Chem. 2003;278:51261-51266. crossref(new window)

Youreva V, Kapakos G, Srivastava AK. Insulin-like growth-factor-1-induced PKB signaling and Egr-1 expression is inhibited by curcumin in A-10 vascular smooth muscle cells. Can J Physiol Pharmacol. 2013;91:241-247. crossref(new window)

Zhang R, Ruan D, Zhang C. Effects of TGF-beta1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations. J Orthop Surg Res. 2006;1:9. crossref(new window)