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In vitro antioxidant and free radical scavenging activities of stem extract of Euphorbia trigona Miller
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  • Journal title : TANG [HUMANITAS MEDICINE]
  • Volume 5, Issue 2,  2015, pp.14.1-14.6
  • Publisher : Association of Humanitas Medicine
  • DOI : 10.5667/tang.2015.0004
 Title & Authors
In vitro antioxidant and free radical scavenging activities of stem extract of Euphorbia trigona Miller
Salar, Raj Kumar; Sharma, Pooja; Purewal, Sukhvinder Singh;
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 Abstract
Antioxidative and free radical scavenging properties of different stem extracts of Euphorbia trigona were evaluated and correlated with its total phenolic content. Aqueous, acetone and methanolic extracts of shade dried stem were obtained and were concentrated in vacuo. The antioxidant and free radical scavenging activities of stem extracts was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, deoxyribose degradation assay and chelating assay. Total phenolic contents (TPC) were evaluated using Folin-Ciocalteu reagent. The results confirmed that the plant is a rich source of polyphenolic compounds which are invariably higher compared to other herbs. All extracts showed TPC in the range of 146.6 - 168.6 mg/g gallic acid equivalents at of extract. Among the three extracts ME showed highest scavenging activity as evidenced by maximum scavenging of DPPH (83.2%), radicals (94.81%), chelating activity (88.59%) and a high reducing power 0.623 at . Our results demonstrate that Euphorbia trigona, an unexplored xerophytic plant could be potential source of natural antioxidants and phytotherapeutic agents. The plant possess invariably high amount of polyphenolic compounds with a broad spectrum of antioxidant properties and could be further used for food, feed and pharmaceutical applications.
 Keywords
Xerophytic plant;phenolic compounds;antioxidants; chelation;reactive oxygen species;
 Language
English
 Cited by
1.
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 References
1.
Aruoma OI, Grootveld M, Halliwell B. The role of iron in ascorbate-dependent deoxyribose degradation. Evidence consistent with a site-specific hydroxyl radical generation caused by iron ions bound to the deoxyribose molecule. J Inorg Biochem. 1987;29:289-299. crossref(new window)

2.
Chizzola R, Michitsch H, Franz C. Antioxidative properties of Thymus vulgaris leaves: comparison of different extracts and essential oil chemotypes. J Agr Food Chem. 2008;56:6897- 6904. crossref(new window)

3.
Conde-Hernandez LA, Guerrero-Beltran JA. Total phenolics and antioxidant activity of Piper auritum and Porophyllum ruderale. Food Chem. 2014;142:455-560. crossref(new window)

4.
Dinis TC, Madeira VM, Almeida LM. Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys. 1994;315:161-169. crossref(new window)

5.
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocher P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006;97:654-660. crossref(new window)

6.
Foti MC, Daquino C, Geraci C. Electron-transfer reaction of cinnamic acids and their methyl esters with the DPPH radical in alcoholic solutions. J Org Chem. 2004;69:2309-2314. crossref(new window)

7.
Hall CA, Cuppett SL: Activities of natural antioxidants. In Antioxidant methodology in vivo and in vitro concepts. Aruoma OI and Cuppett SL eds. (Illinois, USA: AOCS Press), pp. 2-29, 1997.

8.
Halliwell B, Gutteridge JMC, Aruoma OI. The deoxyribose method: A simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals. Analyt Biochem. 1987;165:215-219. crossref(new window)

9.
Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 3rd ed. (Oxford, UK: Oxford University Press), 1999.

10.
Hecker E. New toxic, irritant and cocarcinogenic diterpene esters from Euphorbiaceae and from Thymelaeaceae. Pure App Chem. 1977;49:1423-1431.

11.
Honda K, Casadesus G, Paterson RB, Perry G, Smith MA. Oxidative stress and redox iron in Alzheimer's disease. Ann N Y Acad Sci. 2004;1012:179-182. crossref(new window)

12.
Khare CP. Indian Medicinal Plants: An Illustrated Dictionary.

13.
Khare CP ed. (Berlin, Germany: Springer Science & Business Media), 2007.

14.
Kumar V, Lemos M, Sharma M, Shriramb V. Antioxidant and DNA damage protecting activities of Eulophia nuda Lindl. Free Rad Antiox. 2013;3:55-60. crossref(new window)

15.
Marian V, Leibfritz D, Moncola J, Mark TD, Cronin MM, Joshua T. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39:44-84. crossref(new window)

16.
Nagmoti DM, Khatri DK, Juvekar PR, Juvekar AR. Antioxidant activity and free radical-scavenging potential of Pithecellobium dulce Benth seed extracts. Free Rad Antiox. 2012;2:37-43.

17.
Oyaizu M. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr. 1986;44:307-315. crossref(new window)

18.
Ozsoy N, Cana A, Yanardag R, Akev N. Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chem. 2008;110:571-583. crossref(new window)

19.
Ridnour LA, Isenberg JS, Espey MG, Thomas DD, Roberts DD, Wink DA. Nitric oxide regulates angiogenesis through a functional switch involving thrombospondin-1. Proc Natl Acad Sci USA. 2005;102:13147-13152. crossref(new window)

20.
Robbins RJ. Phenolic acids in foods: An overview of analytical methodology. J Agri Food Chem. 2003;51:2866-2887. crossref(new window)

21.
Salar RK, Certik M, Brezova V, Brlejova M, Hanusova V, Breierova E. Stress influenced increase in phenolic content and radical scavenging capacity of Rhodotorula glutinis CCY 20-2- 26. 3 Biotech. 2013;3:53-60.

22.
Salar RK, Certik M, Brezova V. Modulation of Phenolic Content and Antioxidant Activity of Maize by Solid State Fermentation with Thamnidium elegans CCF 1456. Biotechnol Bioprocess Eng. 2012;17:109-116. crossref(new window)

23.
Salar RK, Seasotiya L, Rohilla SK. Evaluation of antioxidant activity and radical scavenging property of Ficus bengalensis L. applying various spectroscopic and spin-trapping methods. J Biol Active Prod Nat. 2011;1:248-261.

24.
Salar RK, Dhall A. Antimicrobial and free radical scavenging activity of extracts of some Indian medicinal plants. J Med Plant Res. 2010;4:2313-2320.

25.
Salar RK, Seasotiya L. Free radical scavenging activity, phenolic contents and phytochemical evaluation of different extracts of stem bark of Butea monosperma (Lam.) Kuntze. Front Life Sci. 2011;5:107-116. crossref(new window)

26.
Shahat AA, Ibrahim AY, Elsaid MS. Polyphenolic content and antioxidant activity of some wild Saudi Arabian asteraceae plants. Asian Pac J Trop Med. 2014;7:545-551. crossref(new window)

27.
Stohs SJ, Bagachi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med. 1995;18:321-336. crossref(new window)

28.
Surendraraj KH, Sabeena F, Anandan R. Antioxidant Potential of Water Hyacinth (Eichornia crassipes): In Vitro Antioxidant Activity and Phenolic Composition. J Aquat Food Prod Technol. 2013;22:11-26. crossref(new window)

29.
Tada M, Seki H. Toxic diterpernes from Euphorbia trigona (Saiunkaku: an indoor foliage plant in Japan). Agric Biol Chem. 1989;53:425-430. crossref(new window)

30.
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 2006;160:1-40. crossref(new window)

31.
World Health Organization [Internet]. 2008. Traditional medicine. Media centre; [cited 2009 Dec 8]. Available from: http://www.who.int/medicines/areas/traditional/definitions/en/ (accessed on 13th May 2015).

32.
Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agri Food Chem. 1995;43:27-32. crossref(new window)

33.
Yu L, Haley S, Perret J, Harris M. Antioxidant properties of hard winter wheat extracts. Food Chem. 2002;78:457-461. crossref(new window)