Isolation of Histone-type Basic Proteins from Lemna paucicostata

좀개구리밥에서의 Histone 型 鹽基性 蛋白質의 單離

  • Yoon, Joo-Ok (Department of Chemistry, Pusan National University) ;
  • Shin, Hong-Dae (Department of Chemistry, Pusan National University)
  • 윤주억 (釜山大學校 文理科大學 化學科) ;
  • 신홍대 (釜山大學校 文理科大學 化學科)
  • Published : 19641200

Abstract

We isolated histone-type basic proteins from lemna paucicostata for the first time. Basic proteins were extracted directly with dilute mineral acids from homogenized lemna paucicostata. Amino acid compositions of basic protein portions adsorbed on Amberlite CG-50(at pH 6. 0) were resembled to those of calf thymus histones. Especially, lysine content was the greatest of the other amino acids. By chromatographic studies, adsorbed portions of basic protein components on carboxymethyl cellulose column(at pH 4. 2) were shown to be homogeneous to calf thymus histones, however, the area under the individual curve was different, and furthermore, the containing of a non-adsorbed portion in the large extent was markedly different from calf thymus histones. And amino acid compositions of adsorbed portions represented the histone-type basic propertes, but non-adsorbed portions were considered as a different protein compared with the typical histone. When calf thymus histone and protein components separated from lemna paucicostata were heated($60^{\circ}C$) with a solution of $HgSO_4-H_2SO_4$, precipitates were not obtained.

Keywords

References

  1. J. Gen. Physiol. v.30 A. E. Mirsky;A. W. Pollister
  2. Nature v.166 E. Stedman;E. Stedman
  3. 生化學 v.32 土屋敏夫;岩井浩一;安藤銳郞
  4. 生化學 v.35 小林豊;岩井浩一;安藤銳郞
  5. Nature v.189 I. Leslie
  6. J. Gen. Physiol. v.34 M. M. Daly;A. E. Mirsky;H. Ris
  7. Biochem.J. v.77 E. W. Johns;D. M. P. Phillips;P.Simson;J. A. V. Butler
  8. 實驗化學講座 v.23 日本化學會(編)
  9. 蛋白質化學(赤堀${\cdot}$水島編) v.3 安藤銳郞;岩井浩一;山崎誠