- Volume 3 Issue 2
Effects of perchloric acid (PCA) and trichloroacetic acid (TCA) on the color generation by orcinol reaction were systematically investigated. When the concentration of PCA on hot acid treatment was varied from 1 through 5 to 10%, and then the concentration of PCA on orcinol reaction was adjusted to 5% of reaction volume, no difference in the color generation was observed between 5 and 10% of PCA, but clearly observed between 1 and 5% PCA. When RNA was treated in 5% hot PCA and then PCA concentrations on orcinol reaction were adjusted to 5% and 10%, respectively, remarkable differences in color generation were observed. When RNA was treated in 10% hot PCA and then PCA concentration on orcinol reaction was adjusted to 5% no difference in color generation between 5% and 10% hot acid treatment was observed. The results show that PCA concentration must be adjusted prior to orcinol reaction.