Evaluation of Protein Content and Qaulity in Brown Rice by a Dye-binding Method

염색법에 의한 쌀 단백질의 함량 및 질의 평가에 관한 연구

  • Kim, Seung-Kon (College of Agriculture, Seoul National University) ;
  • Lee, Chun-Yung (College of Agriculture, Seoul National University) ;
  • Park, Hoon (Plant Environment Research Institute, Office of Rural Development)
  • 김성곤 (서울대학교 농과대학 농화학과) ;
  • 이춘영 (서울대학교 농과대학 농화학과) ;
  • 박훈 (농촌진흥청 식물환경연구소)
  • Published : 1971.08.28


The dye-binding method based on the reaction of acidic orange-G dye with basic groups of protein molecule was investigated to observe its applicability to the determination of protein, basic amino acid and lysine contents in brown rice of several high-protein rice lines. The protein content of rice samples ranged from 7.91 to 10.53% and from 8.93 to 11.96% in terms of wet and dry bases, respectively. The correlation between dye-binding absorbance and protein content in terms of both dry and wet bases was highly significant; their correlation coefficients being $-0.955^{**}\;and\;-0.975^{**}$, respectively. The correlation of dye-binding absorbance lysine and basic amino acids were highly significant and their correlation coefficients were similar. Dye-binding absorbance-lysine showed a lower correlation than dye-binding absorbance-protein but a higher correlation than protein-lysine.