EVALUATION OF KINETIC PARAMETERS IN ENZYMIC NON-LINEAR PROGRESS REACTIONS

  • Lee Hyun-Jae (Department of Chemistry, University of Colorado Boulder) ;
  • Kim Soo Ja (Department of Chemistry, University of Colorado Boulder)
  • Published : 19710230

Abstract

A modified form of the integrated Michaelis-Menten equation would provide a useful means of evaluating enzyme kinetic parameters in nonlinear progress reaction with time. A slight modification of the Lineweaver-Burk form (and other variants) using for the velocity, the change in substrate concentration divided by time ($\={v}$), and for the velocity, the change in substrate for the time interval ($\={S}$), allows this linear reciprocal form to be used with negligible error even when as much as half of the substrate is utilized during the time interval.

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