Studies on the Production of Lysine by Fermentation Process (2) -Lysine Production by Auxotrophs-

발효에 의한 라이신(L-Lysine) 생산에 관한 연구 (2) -영양요구성 변이주에 의한 Lysine 생산-

  • Min, Tae-Ick (Food Resources Laboratory, Korea Institute of Science and Technology) ;
  • Kim, Hang-Mook (Food Resources Laboratory, Korea Institute of Science and Technology) ;
  • Kwon, Tai-Wan (Food Resources Laboratory, Korea Institute of Science and Technology)
  • 민태익 (한국과학기술연구소 식량자원연구원) ;
  • 김항묵 (한국과학기술연구소 식량자원연구원) ;
  • 권태완 (한국과학기술연구소 식량자원연구원)
  • Published : 1972.06.28

Abstract

Over 90 of lysine producing auxotrophs were obtained from Corynebacterium sp. S-27-12, Brevibacterium flavum ATCC 15168 and Micrococcus glutamicus ATCC 13032 by UV light, $Co^{60}$ irradiation and N-methyl-N'-nitro-N-nitrosoguanidine treatment. One of the mutant, Brev. flavum U46-N59, was identified as a leucine auxotroph and accumulated lysine during flask (500 ml) cultivation (180 strokes/min.) up to 21.6 mg per ml of broth at pH 7.5 and $28^{\circ}C$ after 4 days. The medium consisted of glucose, 100; urea, 10; corn steep liquor, 40; $KH_2PO_4,\;2;\;K_2HPO_4,\;0.5;\; MgSO_4.\;7H_2O,\;0.4;\;antifoam\;S-57,\;1g;\;Fe_2(SO_4)_3.XH-2O,\;10;\; MnCl_2,\;4H_2O,\;10mg;\;biotin,\;30;\;thiamine-HCl,\;100{\mu}g$in 1l of distilled water, and 40 U/ml of penicillin was added after 36 hrs fermentation.

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