Experimental Studies on the Second Intermediate Hosts of Clonorchis sinensis II. Observations on the fate of encysted cercariae of Clonorchis sinensis in the fish hosts, Pseudorasbora parva, Acheilognathus signifer and Acanthorhodeus asmussi

간흡충(肝吸蟲)의 제이중간숙주(第二中間宿主)에 관한 실험적(實驗的) 연구(硏究) Ⅱ. 참붕어, 묵납자루 및 큰납지리 체내(體內)에 있어서 간흡충(肝吸蟲) 피낭유충(被囊幼蟲)의 운명(運命)에 관하여

  • Rhee, Jae Ku (Department of Veterinary Medicine, College of Agriculture Jeonbug National University)
  • 이재구 (전북대학교 농과대학 수의학과)
  • Published : 1973.10.10


The present experiments proposed to pass judgement upon the suitability as the second intermediate host of Clonorchis sinensis, of three kinds of fresh-water fish by having them infected with the cercaria of Clonorchis sinensis and then observing the penetrating ability of the cercaria, and maturity, the process of degeneration and extinction, and infectivity of the metacercaria of Clonorchis sinensis. The following is a brief summary of the leading facts gained through the experiments; 1. P. parva was easily subject to invasion of the cercaria, A. signifer was much less subject to the invasion, and finally A. asmussi was hard to invade. And the infectivity of the cercariae was in proportion to their penetrating ability mentioned in the above. 2. The examination of the cercariae after having three kinds of fresh-water fish infected with them disclosed that 24 hours after the infection, all of the cercariae formed their cysts in muscle and the metacercariae kept growing, and that 7 days later the metacercariae were found folding their bodies twice, and that in 15 days the characteristic structure of the metacercariae was complete and they made a vigorous rotary movement intermittently. 3. Then the metacercariae came to a state of maturity and beyond this stage some metacercariae in P. parva started the process of degeneration and extinction in 133 days; some in A. asmussi, in 140 days; and A. signifer, in 70 days. As more days elapsed, their degeneration and extinction increased in number, and in 269 days all of them in A. signifer became dead while those in A. asmussi were all dead in 460 days. However almost all of them in P. parva survived even after 770 days. The results shown above revealed that P. parva was the most suitable as the second intermediate host among three kinds of fresh-water fish and that A. signifer and A. asmussi were not quite recommendable as the second intermediate host. The ability of the cercaria to invade fresh-water fish, and life span of the metacercaria within fresh-water fish vary outstandingly according to species of fresh-water fish. An explanation as to the mechanism must wait as the subject to be further pursued.