Variation of the Electrophoretic Mobility of Acid Mucopolysaccharides in Polyacrylamide Gel Electrophoresis

Polyacrylamide Gel Electrophoresis에 있어서 酸性 Mucopolysaccharide의 移動度의 變化

  • Geum, Gi-Chang (Department of Biochemistry, School of Medicine, Chungnam National University) ;
  • Ru Do Wi Keu Jul, Ri O (Department of Orthopaedic Surgery, School of Medicine, University of California)
  • Published : 1974.04.30

Abstract

Acid mucopolysaccharides were isolated from nucleus pulposus of whale embryo. The separation of acid mucopolysaccharides was most excellent in $0.03{\%}$ hexamine cobaltic chloride in 0.05 M-sodium acetate buffer solution at pH 4.8. Changes in electrophoretic mobility of acid mucopolysaccharides were observed when the sample solution on top of spacer gel were covered with $40{\%}$ sucrose solution. This effect was not observed by the addition of hexamine cobaltic chloride to the buffer solution.

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