Spectrophotometric Determination of Nitrate with Brucine

Brucine에 依한 窒酸이온의 吸光光度定量法

  • Bo shik Han (Department of Pharmacy, College of Pharmacy)
  • 한보식 (嶺南大學校 藥學大學 藥學科)
  • Published : 1974.12.30


A simple and rapid spectrophotometric method has been found for the determination of nitrate content in natural water according to brucine method by means of neither particular apparatus nor troublesome procedures. Brucine reacted with nitrate in the presence of 30 N sulfuric acid to develop stable color with good reproducibility after 30 minutes reaction at $60^{\circ}C$<\TEX>. An essentially linear relationship to the nitrate concentration was observed in the range of 0.07~0.6 ppm at 410 nm, and it was established that the number of absorbing species in the reaction system was only one between 400 and 490 nm. Among the interferences, nitrite could be removed by the addition of methanol.



  1. J. Amer. Chem. Soc. v.32 E. M. Chamot;D. S. Pratt
  2. Anal. Chem. v.21 A.C. Holler;R.V. Huch
  3. Chem. Zantr. v.23 L.N. Winkler
  4. Soil. Sci. v.57 M. Peech;L. English
  5. Ind. Eng. Chem., Anal. Ed. v.17 C. A. Noll
  6. J. Amer Woter Works Assoc. v.50 A. E. Greenberg(et al.)
  7. Anal. Chem. v.36 D. Jenkins;L.L. Medsker
  8. Standard Methods for the Examination of Water and Sewage American Public Health Association Inc.
  9. Anal. Chem. v.30 F.L. Fisher(et al.)
  10. Japan Analyst v.20 G. Saito(et al.)
  11. Collection Czecho-slov. Chem. Commun. v.24 Z. Deyl;M. Effenberger
  12. J. Phys. Chem. v.64 R. M. Wallace
  13. J. Phys. Chem. v.65 S. Ainsworth
  14. Anal. Chem. v.37 D. Katakis
  15. J. Korean Chem. Soc. v.13 W.K. Park(et al.)
  16. J. Korean Chem. Soc. v.12 K.J. Whang
  17. J. Korean Chem. Soc. v.14 Y. K. Lee
  18. Analyst v.84 J.B.D. Robinson(et al.)
  19. Ind. Eng. Chem, Anal. Ed. v.19 B. Wolf
  20. Chem. Ztg. v.50 L.W. Haase
  21. Anal. Chem. v.32 J. C. Sternberg(et al.)
  22. J. Phys. Chem. v.68 R.M. Wallace;S.M. Katz
  23. J. Korean Chem. Soc. v.16 Y. K. Lee
  24. Inorg. Chem. v.9 J.S. Coleman(et al.)