Study on Improved Diluting Fluids for Direct Counts of Total Leukocyte, Eosinophil and Other Leukocytes

총백혈구수(總白血球數), 호산구수(好酸球數) 및 기타 백혈구수(白血球數)의 직접계산(直接計算)을 위한 개량희석액(改良稀釋液)에 관한 연구(硏究)

  • Lee, Bang Whan (Department of Veterinary Medicine, The City College of Seoul)
  • 이방환 (서울산업대학 수의학과)
  • Published : 1976.10.20


The study was conducted for an attempt to improve the diluting fluid for total leukocyte count, and to prepare a multipurpose diluting fluid for concurrent direct counts of total leukocytes, eosinophils and the other leukocytes. Through the experiment, two better fluids for total leukocyte count of blood of human, bovine, swine, canine and rabbit were selected, and which conserved cell morphology of leukocytes better than $T{\ddot{u}}rk$-solution. Each formula of two fluids were given as under R I and R II. Formula of multipurpose diluting fluid selected in the experiment was given as under III. With this fluid, direct counts of total leukocytes, eosinophils and probably basophils of blood of human, bovine and swine were practicable concurrently in the same counting chamber of a hemocytometer. In this fluid, eosinophils were stained red in the part of eosinophilic granules and blue in other part of cell, and basophils were stained dark blue like as a lump of black granules, staining three other leukocytes faint blue. Eosinophils of canine blood were not so enough red those in other animal and human and eosinophils of rabbit blood were not distinguishable from pseudoeosinophils in this fluid.