The Effect of Coloring Food Additives on the ${\alpha}$-Chymotrypsin Activity

식품착색료가 ${\alpha}$-Chymotrypsin 작용에 미치는 영향

  • Choi Cheong (Department of Food Science and Technology, Yeung Nam University) ;
  • Kim, Sang Ok (Department of Food Science and Technology, Yeung Nam University)
  • 최청 (영남대학교 농축산대학 식품가공학과) ;
  • 김상옥 (영남대학교 농축산대학 식품가공학과)
  • Published : 1977.12.30

Abstract

This study was carried out to understand the activity of ${\alpha}$-chymotrypsin, a proteolytic enzyme, to a oligopeptide in the presence of various coloring food additives. 1. The melting point of synthetic oligopeptide, Asp-Arg-Val-Tyr-Ile-His-Pro-D-Ala, ((8-D-Ala) angiotensin Ⅱ) was 210∼$212^{\circ}C$. Chemical formula and molecular weight were $C_{44}H_{67}N_{13}O_{12}{\cdot}2CH_3COOH{\cdot}H_2O$ and 970.08, respectively. 2.The amino acid rations by acid hydrolysis were Asp : 1.01, Arg : 1.03, Val : 1.00, Tyr :40.94, Ile : 1.00, His : 1.05, Pro : 1.04, D-Ala : 1.03. 3. ${\alpha}$-Chymotrypsin cleaved the oligopeptide bond between tyrosine and isoleucine (Tyr-Ile). 4. The addition of food coloring additives as determined by paper chromatogram, did not influence the inhibitory activity of ${\alpha}$-chymotrypsin on oligopeptide, (8-D-Ala) angiotensin II.

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