Studies on Whole Cell Immobilized Glucose Isomerase - I. Preparation and Properties of Whole Cell Immobilized Glucose Isomerase -

포도당 이성화 효소의 세포 고정화에 관한 연구 - I. 세포 고정화 효소의 제조와 성질 -

  • Ahn, Byung-Yoon (Department of Biological Science and Engineering, The Korea Advanced Institute of Science) ;
  • Byun, Si-Myung (Department of Biological Science and Engineering, The Korea Advanced Institute of Science)
  • 안병윤 (한국과학원 생물공학과) ;
  • 변시명 (한국과학원 생물공학과)
  • Published : 1979.09.15

Abstract

With cells of Streptomyces spp K-45 isolated from soil, the immobilization of glucose isomerase by a series of treatments ; heat, carefully manipulated drying, extrusion with a thickening agent, and glutaraldehyde-induced crosslinking, was presented. This was aimed to obtain a mechanically stable form of whole cell containing glucose isomerase. The resulted pellet form had a good mechanical strength, compared with a commercial product, and showed 26 % of the activity recovery. The specific activity was 48.1 units per g of the dry material. The immobilized glucose isomerase generally showed properties similar to those of the soluble enzyme ; optimal pH at $7.5{\sim}9.0$, optimal temperature at $80{\sim}85^{\circ}C$, activation energy of 10.9 kcal/mole, and $K_m$ for glucose of 10.9M. The immobilized enzyme was very thermostable and pH stable.

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