A Study on the Safety of Stevioside as a New Sweetening Source

신(新) 감미(甘味) 자원(資源) Stevioside의 안전성(安全性)에 관(關)한 연구(硏究)

  • Lee, Sang-Jik (Department of Food and Nutrition, Yeungnam University) ;
  • Lee, Kap-Rang (Department of Food and Nutrition, Yeungnam University) ;
  • Park, Jyung-Rewng (Department of Food and Nutrition, Yeungnam University) ;
  • Kim, Kwang-Soo (Department of Food and Nutrition, Yeungnam University) ;
  • Tchai, Bum-Suk (Institute of Reproductive Medicine and Population, Seoul National University)
  • 이상직 (영남 대학교 식품 영양 학과) ;
  • 이갑랑 (영남 대학교 식품 영양 학과) ;
  • 박정융 (영남 대학교 식품 영양 학과) ;
  • 김광수 (영남 대학교 식품 영양 학과) ;
  • 채범석 (서울대학교, 인구 의학 연구소)
  • Published : 1979.12.15


The safety of the sweetening component of stevia was studied by administrating it to the rats. The $LD_{50}$ determined by intraperitoneal injection was 3,400 mg/Kg as the stevia extract containing 50 % stevioside, i.e. $LD_{50}$ of stevioside was more than 1,700 mg/Kg. Oral administration of large quantities of the stevia extract for 56 days resulted in no effect on the growth of rats. The analyses of total blood (RBC, WBC, Hb and Hct), 17 blood serum components including total protein, glucose, cholesterol, GOT, and 11 items of findings on the liver tissues including nuclear deterioration of liver cells, proliferation of Kupffer cells, fibrosis of portal area showed no significant differences between control and treatments except lactate dehydrogenase activity after 56 day-oral administration of the extract. From the results obtained, it was supposed that the stevia extract/stevioside revealed no acute or sub-acute toxic effects on rats.