# In Vitro에 의한 유황피복요소(硫黃被覆尿素)와 Diuredio Isobutane의 이용효과(利用效果) 판정(判定)

• Lee, Ho-Il (Department of Veterinary Medicine, College of Agriculture, Jeonbug National University) ;
• Rhee, Jae-Ku (Department of Veterinary Medicine, College of Agriculture, Jeonbug National University) ;
• Choi, In-Hyuk (Department of Veterinary Medicine, College of Agriculture, Jeonbug National University)
• Published : 1982.10.09

#### Abstract

In order to explore more efficient protein substitutes by improving the utilization of non-protein nitrogen compounds in ruminants, the experiment was undertaken. The effects of sulfur coated urea (SCU) and diuredio isobutane (DUIB) in the ruminal fluid on the concentration of $NH_3-N$, the total count of the ruminal ciliates and pH value were estimated in vitro. The results obtained from the experiment were as follows: 1. The pH of the media of the group added diuredio isobutane and sulfur costed urea tends to decrease slightly at 9 hours after the incubation, but no pH changes were observed in the media added urea alone. 2. The number of the ciliates in the ruminal fluid was slightly increased 9 hours after the incubation in all groups. 3. The concentrations of $NH_3-N$ in the ruminal fluid were gradually increased according to the incubation in vitro showing 418, 431 and $627{\mu}g/ml$ in the group added diuredio isobutane and 428, 569 and $792{\mu}g/ml$ in the group added sulfur coated urea at 0, 0.5 and 9 hours after the incubation, respectively.