Study on the Cultural Conditions of Starch Utilizing Yeast Sporobolomyces holsaticus

전분이용성효모, Sporobolomyces holsaticus FRI Y-5의 배양조건에 관한 연구

  • 박완수 (농촌개발공사 식품연구소) ;
  • 구영조 (농촌개발공사 식품연구소) ;
  • 신동화 (농촌개발공사 식품연구소) ;
  • 민병용 (농촌개발공사 식품연구소)
  • Published : 1983.01.01


It was investigated under several cultural conditions to produce biomass directly from starch by an strictly aerobic and amylolytic yeast, Sporobolomyces holsaticus FRI Y-5. Its optimal temperature and initial pH of medium for growth were $23^{\circ}C$ and 6.9, respectively. Activation energy, Ea, for growth was calculated to be 17.33 Kcal/mole from the Arrhenius relationship. When each of 13 nitrogen sources was added to the basal medium, $(NH_2)_2CO$ had the best effect, on which concentration of cell after 3 day incubation was 10.6 g/l and cell yield was 0.451. The yeast growth was affected by $MgSO_4,\;K_2SO_4\;and\;ZnSO_4$ as a mineral source and was best on the medium containing all of them. The addition of yeast extract (5g/l) could enhance the production of biomass and cell yield to 77% and 32%, respectively.