Study on the Pattern of Starch Assimilation by Sporobolomyces holsaticus

Sporobolomyces holsaticus의 배양중 전분자화 특성조사

  • 박완수 (농어촌개발공사 식품연구소) ;
  • 구영조 (농어촌개발공사 식품연구소) ;
  • 신동화 (농어촌개발공사 식품연구소) ;
  • 민병용 (농어촌개발공사 식품연구소)
  • Published : 1983.01.01


Direct conversion of starchy materials to single cell protein of Sporobolomyces holsaticus FRI Y-5 was investigated. Effect of yeast extract concentration on its cell growth showed that it could utilize more of starch in the medium containing 2.5 g/l of yeast extract. In case of jar fermentor culture, the specific growth rate and cell yield of Sp. holsaticus on soluble starch were calculated to be $0.14\;hr^{-1}$ and 0.425, respectively and its maximum cell concentration was 13.4 g/l. After 80 hr of incubation time, 45.96% of starch was consumed and 45.1% of relative blue value was decreased. Reducing sugars in the starch medium seemed to increase from 4.06 g/l to 6.08 g/l and then to decrease. During fermentor culture, pH of medium was almost not changed in the range of $pH\;7.0{\pm}0.5$. The optimal temperature and pH of Sp. holsaticus amylase activity were $40^{\circ}C$ and pH 7.5, respectively. It was shown from the effect of Tapioca starch concentration on the cell growth that the optimal concentration of Tapioca starch for Sp. holsaticus was lower than that of soluble starch. FRI Y-5 cells settled much slower than Sp. holsaticus IFO 1032 cells and the viscosity vs cell concentration relationship was related to be linear.