A Simple and Modified Photometric Method for Measuring Lipase Activity

리파제 활성측정을 위한 간편한 비색정량법

  • Kim, Chul-Jin (Department Biological Science and Engineering, Korae Advanced Institute of Science and Technology) ;
  • Cheigh, Hong-Sik (Department of Food Science and Nutrition, Pusan National University) ;
  • Byun, Si-Myung (Department Biological Science and Engineering, Korae Advanced Institute of Science and Technology)
  • 김철진 (한국과학기술원 생물공학과) ;
  • 최홍식 (부산대학교 식품영양학과) ;
  • 변시명 (한국과학기술원 생물공학과)
  • Published : 1984.06.30

Abstract

A simple and modified method is developed to determine a lipase activity. A linear relationship exists between the color intensity and the free fatty acid liberated by enzyme action. The range of determination is from 0.05 to $1.5\;{\mu}moles$ of long chain fatty acid (oleic acid) and 0.2 to $2.0\;{\mu}moles$ of short chain fatty acid (caproic acid). The cumbersome procedure of the removal of the upper aqueous phase which was required in the previous copper soap extraction method was eliminated by the movement of solvent phase to upper phase in the respective biphasic system with a mixture solvent (chloroform: n-hexane:ethanol = 49:49:2) and copper reagent saturated with sodium chloride.

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