Isolation and Identification of Mycobactin Dependent Acid-fast Bacteria (M. paratuberculosis) from Bovine Fecal Material

우유래(牛由來) Mycobactin의존성(依存性) 항산성세균(抗酸性細菌) (M. paratuberculosis)의 분리동정(分離同定)

  • Jeon, Yun-seong (College of Veterinary Medicine, Seoul National University) ;
  • Lee, Bang-whan (Department of Veterinary Medicine, College of Agriculture, Chonnam National University) ;
  • Kim, Jong-bae (College of Veterinary Medicine, Seoul National University) ;
  • Choi, Chul-soon (College of Medicine, Chung-Ang University) ;
  • Kim, Jin-koo (Green Cross Veterinary Products Co., Ltd.)
  • 전윤성 (서울대학교 수의과대학) ;
  • 이방환 (전남대학교 농과대학 수의학과) ;
  • 김종배 (서울대학교 수의과대학) ;
  • 최철순 (중앙대학교 의과대학) ;
  • 김진구 (녹십자수의약품주식회사)
  • Received : 1984.03.13
  • Published : 1984.06.01


Fecal material from cattle, which was confirmed to be infected with Johne's disease by clinical and pathological symptoms, was decontaminated with 4% NaOH and inoculated into the $L{\ddot{o}}wenstein$-Jensen media supplemented with 1% of heat-killed Mycobacterium bovis. After 2-4 week-incubation at $37^{\circ}C$, typical acid-fast mycobacteria was isolated. With the results of staining properties, morphological characteristics, the requirement of mycobactin for growth and the other biochemical properties, isolated mycobacteria was identified as Mycobacterium paratuberculosis. Female guinea pigs were sensitized with the isolates, and skin test was done with purified protein derivatives (PPDs) of M. avium, M. bovis and M. paratuberculosis 4 weeks after sensitization. Animals showed the largest reaction to the PPDs of M. avium and M. paratuverculosis.